HVT细胞株|人绒毛间叶纤维细胞系技术优势大 货号:GD-C9919649
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Myricitrin
cis-5-Norbornene-endo-2,3-dicarboxylic anhydride
3-BroMo-1H-indazole-5-carboxaldehyde
2-AMino-5-broMo-6-Methylpyridine
4-Methoxyisophthalonitrile
N,N-DiMethyl 2-(2-broMophenyl)acetaMide
2-Ethylaminoethylamine
Nicotinic Acid N-Oxide
6-Amino-4-hydroxy-2-naphthalenesulfonic Acid
1-Benzyl-4-hydrazinopiperidine trihydrochloride
(1S, 2S)-(1-BENZYL-3-CHLORO-2-HYDROXY-PROPYL)-CARBAMIC ACID TERT-BUTYL ESTER
2-METHYL-1,3-DITHIANE
Methyl 1,2,4-triazole-3-carboxylate
Chloromethyltrimethoxysilane
3,5-Di-tert-butylsalicylaldehyde
PotassiuM phosphate
Ethyl 2-Ethyl-2-Methylacetoacetate
3-Pyrid-3-ylbenzaldehyde
4-Hexyloxybenzaldehyde, 99%
CARBOSULFAN
Isopropyl Hexanoate
Cyclopentylacetic acid
Lead fluoride
2-(2-Ethoxyphenyl)-4,5-diphenylimidazole-1,2'-dimer
3,5-Dinitrotoluene
4-METHYLCYCLOHEXYLAMINE HYDROCHLORIDE
3-METHOXYPHENYLPROPARGYL ALCOHOL
4-Chlorobenzoylacetonitrile
Methyl 2-broMothiazole-4-carboxylate, 96%
tert-Butyldiphenylchlorosilane, 97%
Xanthophyll
2',3,3',4,5,6-HEXACHLOROPHTHALANILIC ACID
4-METHYL(THIOBENZAMIDE)
N,N-DIISOPROPYLBENZAMIDE
3-BroMo-2-phenylpyridine
TetrabutylaMMoniuM perchlorate
Pararosaniline chloride, high purity biological stain, pure
5-(2-Iodophenyl)-1H-tetrazole
(4-bromobenzyl)ethylamine(SALTDATA: HCl)
n-Hexanal dimethylacetal, 98+%
2-Pentanol
Calcium phenylpyruvate
Methyl vinyl ketone
D-Tyrosine;D-α-AMino-β-p-hydroxyphenyl propionic acid;D-α-AMino-p-hydroxyhydrocinnaMic acid;?-2-AMino-3-(4-hydroxyphenyl)propionic acid;R(+)-Tyrosine;3-(4-Hydroxyphenyl)-D-alanine
Tropaeolin O
4-(4-Hydroxyphenyl)cyclohexanone
1-[4-(2-Phenylethyl)benzyl]naphthalene
(ChloroMethyl)triphenylphosphoniuM chloride, 95%, tech.
3-Benzyloxyaniline, 98%
Methyl thiobutyrate
(-)-trans-1,2-Cyclohexanedicarboxylic Anhydride
5-Azaadamantan-2-one
Acid Red 251
beta-D-Maltose octaacetate
5-Deoxy-D-ribose
2-(TRIMETHYLSILYLETHYNYL)PYRIDINE
2,2-DI-N-OCTYL-1,3-PROPANEDIOL
3-n-Hexylthio-1,2,4-triazole, 97%
3-Fluoro-4-nitroanisole
1,7-Dihydroxynaphthalene
2-Bromo-1-(3,4-dihydro-2H-1,5-benzodioxepin-6-yl)-1-ethanone, 97%
2-{4-[4-(Trifluoromethyl)pyrimidin-2-yl]piperazino}ethan-1-ol , 97%
2-Fluoro-4'-methoxy-3'-nitrobenzophenone
Methyl 4-trifluoromethylbenzoate
3-CYANOCOUMARIN
2-Methyl-5-methoxythiazole
2,4,6-Trifluorophenol
2-BroMo-4'-phenylacetophenone
2,2-Bis(3-aMino-4-Methylphenyl)hexafluoropropane
Ethyl 5-(4-chlorophenyl)oxazole-4-carboxylate
alpha,alpha,alpha-Trifluoro-p-tolyl isocyanate, 99%
CarMinic acid, 70-90%
3,4-Dichlorothiophene 1,1-Dioxide
N-VinylacetaMide
Benzoylformic acid
6,8-difluoro-3,4-dihydro-2H-1-benzopyran-4-one
Tribulus terrestris fruit
4-(1H-tetrazol-1-yl)phenol
F1+2 ELISA Kit
BAFF ELISA Kit
Amylin ELISA Kit
Human Resistin ELISA Kit
MHC I/H-2 II ELISA Kit
Rat Interleukin-7,IL-7 ELISA Kit
T ELISA Kit
CHRM1 ELISA Kit
Human trypsin ELISA Kit
Mouse cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit
Charcoal Agar
Charcoal CefalexinBlood Agar Base
Ethyl 4,8-dichloroquinoline-3-carboxylate, 97%
trans-4-[4-(4-n-Pentylcyclohexyl)phenyl]benzonitrile, 99%
4-AcetaMidoantipyrine, 97%
2-AMinoterephthalic acid, 99%
Cyclopentylacetic acid
Naltrexone
4-Bromopyridine N-Oxide
3-Hydroxybenzyl alcohol
Mitomycin C
Lipase
S-Trityl-3-Mercaptopropionic Acid
Methyl 3,5-dibroMo-2,4-diMethoxy-6-Methylbenzoate, 98+%
AdaMantane-1-carboxaMidoxiMe, 97%
Ethyl 3-hydroxybenzoate
4-Chloro-2-(trifluoroMethoxy)aniline
Boc-L-2-indanylglycine
4'-Fluoro-2'-hydroxyacetophenone
3-METHYLPHENOXYACETIC ACID
3-ForMyl-4-Methylphenylboronic acid
4-Bromobenzamidine hydrochloride
Ethyl 2-chloro-2-[2-(4-chlorophenyl)hydrazono]-acetate
2-tert-Butoxy-6-bromopyridine ,97%
3-Chlorosulfonyl-benzoic acid methyl ester