Hurt-78细胞株|人T淋巴瘤细胞系技术优势大 货号:GD-C9919648
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-BroMobutyric acid
PotassiuM hydroxycitrate tribasic Monohydrate
6-AMINOCHRYSENE
4-(DiMethylaMino)cinnaMic acid
1-Nitropentane
L-Arginine ethyl ester dihydrochloride
Diethyl aminomalonate hydrochloride, 98%
3,3'-Bis(trifluoromethyl)benzophenone
4-hydrazino-5,6-dimethylthieno[2,3-d]pyrimidine(SALTDATA: FREE)
3,4-Difluorothiophenol 97%
2-Methoxybenzonitrile
Aluminium bromide
1,4-Bis(vinyloxy)-butane
3-Hexylthiophene
N-Methyl-N-(4-pyridinylMethyl)aMine
TripropylaMine
4-Ethylphenetole
4-(Pyridin-3-yloxy)benzaldehyde
(1S,4R)-N-FMOC-1-AMinocyclopent-2-ene-4-carboxylic acid, 95%, 98% ee
CARBON TETRACHLORIDE
4-Octanone
Cyclopentylacetone, Pract.
Magnesium fluoride
3,3,4,4-TETRAFLUOROPYRROLIDINE HYDROCHLORIDE
3-Amino-5-nitrobenzoic acid
ELLAGIC ACID DIHYDRATE
4'-tert-Butylacetophenone
BariuM hydrogen phosphate
SodiuM 1-pentanesulfonate, 99% (dry wt.), water <1%.
4-BroMoMandelic acid, 98+%
Dieldrin solution
5-(3-Chlorophenyl)oxazole-4-carboxylic acid
()-2,2-DibroMo-1,1-binaphthyl, 96%
N,N-DiMethyl 3-broMo-5-trifluoroMethylbenzenesulfonaMide
ChlorodiMethylpentafluorophenylsilane
PHTHALYL-DL-GLUTAMIC ACID
3-Isopropenyl-alpha,alpha-dimethylbenzyl Isocyanate
2-Amino-2-methylpropionitrile
N-Benzylquininium chloride ,98%
Ethyl 2-(trifluoromethyl)benzoate 97%
5-Bromo-2,3-difluoroanisole
3-Methylquinoline
L-Alanyl-L-leucine
Loratadine;
3-(2-Furyl)-1H-pyrazol-5-aMine
DiaMylaMMoniuM Acetate (ca. 0.5Mol/L in Water) [Ion-Pair Reagent for LC-MS]
1-BOC-5-(Methoxycarbonyl)pyrrole-2-boronic acid
4-Picoline, 99%
1-Hexyl-3-MethyliMidazoliuM tetrafluoroborate, 98%
8-Hydroxyquinoline sulfate
2-Chloro-5-fluoropyridine
Isopropylantipyrine
4-chloro-4'-propylbi(cyclohexane)
methyl 5-aminolevulinate hydrochloride
Methyl 4-(benzyloxy)-3-methoxybenzoate
2-FLUOROBENZOTRICHLORIDE
Ethyl (3-boronobenzoylaMino)acetate
TRANS-5-NORBORNENE-2,3-DICARBONYL CHLORIDE
1-Methoxy-2-naphthoic acid
2,3-Dihydro-benzo[1,4]dioxine-2-carboxylic acid ethyl ester
Phenyl beta-D-Glucopyranoside Hydrate
N-Methoxy-N-methylacetamide
4-Bromo-2-iodotoluene
CHLORODIFLUOROACETAMIDE
1,4-BIS(TERT-BUTOXY)TETRAFLUOROBENZENE
Ethyl L(-)-lactate
Magnesium silicate
3-(DiMethylaMino)propyl Chloride Hydrochloride
2,2-Bis(3-aMino-4-hydroxyphenyl)hexafluoropropane
AMyl Laurate
5-Fluoro-1h-pyrrolo[2,3-b]pyridin-2(3h)-one
1-BroMoisoquinolin-3-aMine, 97%
4,6-DIOXOHEPTANOIC ACID
5-BroMo-4-chloro-3-indolyl beta-D-Glucuronide SodiuM Salt [for BiocheMical Research]
Neoisoliquiritin
2,4,6-trichloro-benzaldehyde-oxime
Acid Green 27
4-Fluoro-2-nitrobenzaldehyde
F5-Ag ELISA Kit
Mouse BAFFR (B-Cell Activation Factor Receptor) ELISA Kit
Human Amylin ELISA Kit
Human Resistin ELISA Kit
MHC I/H-2 III ELISA Kit
Rat Interleukin-7,IL-7 ELISA Kit
SCF ELISA Kit
Human TSST-1 ELISA Kit
Human TRY (Trypsin) ELISA Kit
Mouse cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit
Thiobacillus Ferrooxidans Medium
Azospirillum Amazonense Medium
Ethyl 4-[4-(2-broMobenzyl)-1-piperazinyl]benzoate, 97%
trans-4-n-Propyl-4'-(4-n-propylcyclohexyl)biphenyl, 98%
4'-AMinoacetanilide, 98+%
Ethyl 2-AMinooxazole-4-Carboxylate
Cyclopentylacetone, Pract.
Sodium methylsiliconate
N-(1-oxooctyl)glycine
m-Tolualdehyde
1-DODECANETHIOL
TAU-FLUVALINATE
5-Chloropyrazine-2-Carboxylic Acid
4,5-Difluoro-2-iodobenzoic acid, 98%
Trifluoroacetaldehyde hydrate, tech., ca 75% in water
PotassiuM bis(triMethylsilyl)aMide
3-AzetidineMethanol hydrochloride
5-CYANO-2-METHYLPYRIDINE
N-(5-BROMO-3-METHYLPYRIDIN-2-YL)-2,2-DIMETHYLPROPANAMIDE
4-BENZYLOXY-2-CHLOROPYRIMIDINE
4-(2-THIAZOLYLAZO)RESORCINOL
2-Bromo-2-methylpropionyl bromide, 98%
2-Chloronicotinic acid ,99%
2,4,5-Trihydroxybenzaldehyde, 99%
4-Bromo-2-methylbenzaldehyde ,98%