HE-MEF细胞株|小鼠胚胎成纤维细胞系技术优势大 货号:GD-C9920157
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Fluoro-4-Methoxybenzyl alcohol, 98%
FMoc-Phe(4-Br)-OH
2-(p-Tolyl)benzoic Acid , 98%
BIS[2-(DIBUTYLAMINO)ETHYL]AMINE
3-BroMo-6-fluoro-2-Methylpyridine
2'-O-Methyluridine
AMbroxol hydrochloride
Phosphorus, red, powder, pure, 97%
h-d-pro-ptbu.hcl
4-[4-Chloro-3-(trifluoromethyl)phenyl]-4-piperidinol, 97%
2,4-Dichlorobenzhydrazide
5-Chloro-2-methoxyphenylboronic acid
3-Fluorobenzotrifluoride
YTTERBIUM CHLORIDE
1-(TrifluoroMethyl)cyclopropane-1-carboxylic acid Methyl ester
BariuM sulfate
1,1'-(Decane-1,10-diyl)bis[4-aza-1-azoniabicyclo[2.2.2]octane] DibroMide
N,N-Diisopropyl 4-broMo-3-MethoxybenzaMide
trans-Zeatin (synthetic), 97%
Polydextrose, Untreated, FCC
2-HydroxyisobutyraMide
4-Ethylaniline
2-Iodo-4-nitroanisole
Di-tert-butyl azodicarboxylate
4-Amino-3-trifluoromethylbenzonitrile
TRIS(CYCLOPENTADIENYL)NEODYMIUM
2-Pentylpyridine
N-(4-Ethoxyphenyl)MaleaMic acid, 97%
2,4,4,6-TetraMethyl-1-oxa-3-aza-2-cyclohexene, 95%
2-Methylcyclopentane-1,3-dione, 98%
Z-Aib-OH
EDTA TETRASODIUM SALT: HYDRATE
6-Fluoro-1H-indazole
PROPYLMAGNESIUM CHLORIDE
trans-3-Chloro-β-nitrostyrene
ALPHA-BROMO-4-CHLOROPHENYLACETIC ACID
2-Chloro-6-(trifluoromethyl)nicotinonitrile
3-Methyl-1 H -pyrazole-4-carboxylic acid
4-Bromo-2-difluoromethyl-1-fluorobenzene
2-Propyl-4-(4-pyridylazo)phenol
Ceric ammonium nitrate
3,7-DIMETHYL-1-OCTANOL
TRANS-5-DECEN-1-OL
2,3,6-TriMethylphenol;1-Hydroxy-2,3,6-triMethylbenzene;
PhenoxytriMethylsilane
Bis(phenylacetyl) Disulfide
2-EthylaMinopyriMidine-5-boronic acid, pinacol ester
DichloroMethylphenylsilane, 98%
ThuliuM(III) oxide, 99.9%
(S)-2,2'-DiMethyl-1,1'-binaphthyl
Ethyl 2-Thiophenecarboxylate
1,1'-diethenyl-Ferrocene
3-Bromo-2-chloro-6-pyridinecarboxylic acid
bis(4-(3-aminophenoxy)phenyl) phenylphosphine oxide
2,5-Difluorobenzylbromide
POTASSIUM TUNGSTATE
(S)-(-)-2-Guanidinoglutaric Acid , 98%
N-(1-Pentyl)forMaMide
Anisonitrile
5-Bromo-2,1,3-benzoxadiazole
5-Hydroxyindole
Di-tert-butyl iminodicarboxylate, 97%
Boc-trans-4-aminocyclohexane acetic acid
N-Carbobenzyloxy-L-alanine
Phenyltriethoxysilane
Emedastine
Polydextrose
Cibacron? Brilliant Yellow 3G-P
1,4-Bis[2-(4-Methyl-5-phenyloxazolyl)]benzene [for scintillation spectroMetry]
3-BroMo-2-chloropyridine-4-boronic acid
5-Fluoroindole, 98%
Oxolinic acid, 98%
H-TYR-GLY-OH
Hydroxychloroquine Sulfate
Fraxin
Carboxymethylcellulose sodium
5-(4-Methoxy-2,3,6-Triphenyl)-3-Methyl-2,4-Pentadiene-1-Bromidetriphenylphosphine
4-Hydroxy-2,6-dimethylbenzonitrile
FMOD ELISA Kit
Aβ1-42 ELISA Kit
CENPH ELISA Kit
Human secretory component,SC ELISA Kit
Chicken Thyroxine,T4 ELISA Kit
PL12/AlaRS ELISA Kit
Chicken β-lactamase ELISA Kit
Human TEF (Thyrotroph Embryonic Factor) ELISA Kit
Human ANG I (Angiotensin I) ELISA Kit
Fb ELISA Kit
Anaerobic Egg Yolk Agar Base
Meclary Agar
Isoquinoline N-oxide, Min 50% w/v in water
NeodyMiuM(III) perchlorate, 50% w/w aq. soln., Reagent Grade
5-Chlorovaleronitrile, 97%
2-FLUORENECARBOXALDEHYDE
4-Ethylaniline
N'-(3-aminopropyl)-N,N-dimethylpropane-1,3-diamine
Bismuth trichloride
Decanoic acid
2,3,5,6-Tetramethyliodobenzene
4-Iodopyrazole
Methyl 5-chloroanthranilate
(1S,2S)-2,5-Diazabicyclo[2.2.1]heptane dihydrobroMide, 98%
2-Chloro-5-fluorobenzeneboronic acid, 98%
1,4-Bis(2-Methylstyryl)benzene
Isopropyl cyanoacetate
N-Methyl-N-(2-hydroxyethyl)-4-aMinobenzaldehyde
Tetraethylene glycol diMethacrylate
2-(2-BroMophenyl)propanenitrile
5-Chloro-2-ethoxy-3-nitropyridine
2-Bromo-isonicotinamide
Ethyl oxamate, 99%
4-Ethyltoluene
Galanthamine.HBr