H-4-Ⅱ-E细胞株|大鼠肝癌细胞系技术优势大 货号:GD-C9920096
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Chloro-2-Methoxypyridine-4-boronic acid, 95%
3,4-DiMethoxycinnaMonitrile, predoMinantly trans
3-Chloro-alpha-Methylbenzyl Alcohol , 98%
trans-4-(t-ButyldiMethylsilyloxy)-1-buten-1-ylboronic acid, pinacol ester
4-ISOPROPYLCYCLOHEXANONE
N-(1-Methylethyl)-benzeneMethanaMine
2-Octadecanone
N-(Chloroacetyl)-4-methylaniline
Ethyl 2-Mercaptopropionate
2-(chloromethyl)-6-methylimidazo[1,2-a]pyridine(SALTDATA: HCl)
2-bromo-N-isopropylacetamide(SALTDATA: FREE)
1-(Bromomethyl)naphthalene
3,4-Diethoxybenzoic acid
2,6-Dichloroaniline
Methyl L-histidinate dihydrochloride
2,5-Dihydroxybenzoic acid
1,1'-DinaphthylaMine
2,4-DibroMo-6-Methoxyaniline
TitaniuM(IV) oxide, 98+%, anatase powder
Periodic Acid, ACS
Bis(4-fluorophenyl) Ether
Tolylene-2,4-diisocyanate
1-(1'-Methylethyl)-4-piperidone
7-hydroxy-1,3-naphthalenedisulfonic acid, dipotassium salt
Hydroquinone Dimethyl
DICHLOROISOBUTYLMETHYLSILANE
THIAMPHENICOL
2,6-Dichlorobenzoxazole
SodiuM 1-heptanesulfonate Monohydrate, 99%
Xanthone, 99%
ANTU
Flutolanil
O-ISOBUTYLHYDROXYLAMINE HYDROCHLORIDE
Ribose
1-(2-Methoxyphenyl)-3-buten-1-ol
1,3-DIIMINOBENZ[F]ISOINDOLINE
Bromotrichloromethane
4-(Trifluoromethyl)benzenesulphonamide
1-(4-Methoxyphenyl)-1-cyclopropanecarbonitrile, 96%
4-Fluoro-3-(trifluoromethyl)phenylboronic acid pinacol ester
BROFOXINE
3,4-DINITROTOLUENE
5-BENZYLOXY-2-BROMOTOLUENE
(+)-1-(9-FLUORENYL)ETHYL CHLOROFORMATE
Hypophosphorous acid
Isobutyl 4-AMinobenzoate
3-Methyl-1h-pyrazolo[3,4-b]pyridine
3-Acetylpyridine, 98%
N-Methyl-N-(3-pyridin-3-ylbenzyl)aMine, 97%
Sucralfate
1-Methoxyphenazine
Sudan IV
(3beta,16beta,21beta,22alpha)-28-(acetyloxy)-16,22,23-trihydroxy-21-{[(2E)-2-methylbut-2-enoyl]oxy}olean-12-en-3-yl beta-D-glucopyranosiduronic acid
O-nitrophenyl myristate
Silicristin
4-Ethoxycarbonylphenylboronic acid
6-METHYL-4-(TRIFLUOROMETHYL)-2(1H)-PYRIDONE
3-Chloro-5-(trifluoroMethyl)acetophenone, 97%
1-HEPTYN-3-OL
4-Methylumbelliferyl-alpha-D-glucopyranoside
Dimethoxymethane
1-Benzoyl-3,5-bis(trifluoromethyl)pyrazole
2-(1-Methylhydrazino)quinoxaline
LEAD ZIRCONATE
Phytic acid
POTASSIUM SELENATE
Aliquat 7
3-Chloro-1,2-benzisoxazole
1,3-Cyclohexanediol, Mixture of cis and trans
Methyl 2,3,4-Tri-O-benzyl-1-thio-beta-L-fucopyranoside
2-(Pyrazol-1-yl)-3-cyanopyridine
2-AnilinopyriMidine-5-carboxylic acid, 97%
PHOSPHORUS PENTABROMIDE
1,1,1,2,2,3,3-Heptachloropropane
bis(pentane-2,4-dionato-O,O')zinc
5,6,7,8-Tetrahydroquinoxaline
Vitamin K
Asarone
HFABP ELISA Kit
BLI ELISA Kit
Human LAM ELISA Kit
Human Blocking antibody,BA ELISA Kit
IgA ELISA Kit
M2-PK ELISA Kit
Chicken 17-ketosteroids,17-KS ELISA Kit
E ELISA Kit
Human vasopeptidase inhibitors,VPI ELISA Kit
SEPP1 ELISA Kit
Sabouraud-glucose Agar with antibiotics
Egg-Yolk Manitol Salt Medium Base
Isonicotinic acid
StrontiuM zirconiuM oxide, 99.3% (Metals basis)
5-AMino-2-fluorobenzoic acid, 99%
2-Hexyn-1-ol, 97%
Tolylene-2,4-diisocyanate
Zinc iodide
2-Chloro-4,6-di(morpholin-4-yl)-1,3,5-triazine
3-Amino-1-propanol
ALLYL PHENYL SULFIDE
Trimethylsilyl trifluoromethanesulfonate
2-BroMo-4,5-diMethoxybenzonitrile, 99%
Isoquinoline-5-sulfonic acid, 95%
Ethyl 4-chloro-5,8-diMethoxyquinoline-3-carboxylate, 97%
6-α-Methylprednisolone Solution
2,4-Difluoro-3-Methylbenzonitrile, 99%
N-(Furan-2-ylMethyl) 3-broMo-5-MethylbenzenesulfonaMide
3-AMino-4-chloropyridine
4-Fluoro-5-Methoxy-2-nitroaniline
TRI-O-BENZYL-D-GLUCAL
4,4'-Diiodo-3,3'-dimethylbiphenyl
2-Bromo-5-chloronitrobenzene
(R)-2-Methylpiperidine
2-Acetamido-4-methyl-5-thiazolesulfonyl chloride, 97%