Mv.1.Lu细胞株|貂肺上皮细胞系技术优势大 货号:GD-C9919292
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-BroMo-4'-nitroacetophenone, 95%
[6]-Gingerol
trans-1,3-Dichloropropene
N3-BENZYL-2-CHLORONICOTINAMIDE
N-(2-CYANOETHYL)-N-(2-HYDROXYETHYL)-M-TOLUIDINE
(Perfluoroisopropyl)phenyliodoniuM TrifluoroMethanesulfonate , 97%
Trans-2-(3-Methoxyphenyl)vinylboronic acid pinacol ester
2-bromo-6-t-butylbenzothiazole
Mesitylcopper(I), min. 97%
Anthraquinone ,98%
3,4-Dihydroxybenzyl Alcohol
CADMIUM HYDROXIDE
1,3-Dimethyl-5-phenylbarbituric acid
PTH-GLYCINE
(5-BroMo-3-benzo[b]thienyl)Methanol
1-Dodecyne
2,2-DiMethylbutyryl Chloride
Methyl 4-broMo-1-naphthoate
1-Hydroxy-2,2,4,5,5-pentaMethyl-3-iMidazoline-3-oxide
TBHQ, FCC
4,4'-(Hexafluoroisopropylidene)diphthalic Anhydride
2-methyltricyclo[3.3.1.1~3,7~]decane
Mordant yellow 8 (C.I. 18821)
Tri(Hydroxymethyl) Amino Methane Hydrochloride
P-Hydroxybenzene propanoic acid
2,4-Difluorobenzenethiol
2-AMino-5-Chlorobenzoic Acid
Methyl 2-oxoindane-1-carboxylate, 97%
Triphenylphosphine dichloride, 96%
tert-Butylhydrazine hydrochloride, 98%
Dodecanedioic acid
delta-Tridecanolactone , 98%
4-BroMo-3-isopropyl-1H-pyrazole
N-tert-ButylacetaMide, 98%
4-BroMo-6-Methyl-2-(trifluoroMethyl)quinoline
DIHYDROERGOTAMINE MESYLATE
3-Bromo-5-methoxy-4-propoxybenzonitrile
amino(3,5-dimethyl-1H-pyrazol-4-yl)acetic acid(SALTDATA: FREE)
Fmoc-Lys(Ac)-OH
2,3-Dihydro-6-isothiocyanato-1,4-phthalazinedione [HPLC Labeling Reagent for Chemiluminescent]
Acid Yellow 23
3-Phenylpyridine
TRISTEARIN
L-PHENYLALANINE
Bis-(4-hydroxy-1-naphthyl)phenylMethanol
Ethyl 11-BroMoundecanoate
2'-Fluorobiphenyl-3-carboxylic acid
BroMelain, pure, 1200 GDU,g
2-Phenylthiophene, 96%
2,6-DiaMinopyridine
6-AMino-1,3-diMethyluracil
Allyltrimethylsilane
(R)-3-Benzylmorpholine
phosphomycin calcium
3-(2,4-Dinitrophenylamino)propyltriethoxysilane
2,4-Difluoroanisole
N-TERT-BUTYLBENZENESULFENAMIDE
TRIMETHYL(2-PYRIDYL)TIN
4-(Methylphenyl)sulfonylacetonitrile
1-[(2-methyl-1,3-thiazol-4-yl)methyl](SALTDATA: FREE)
Ethyl 5-(2-bromoacetyl)isoxazole-3-carboxylate
2,5-Dimethoxy-2,5-dihydrofuran (cis- and trans- mixture)
1,4-Bis(3-hydroxyphenoxy)benzene
1-(2,3-Dimethylphenyl)
1-BROMO-2,4,6-TRIMETHOXYBENZENE
CADMIUM SULFATE
1,3-Diethylbenzene;M-Diethylbenzene;
2,4,6-Collidine
1-Butyl-3-MethyliMidazoliuM TribroMide
3-(2-Ethoxy-2-oxoethoxy)phenylboronic acid, pinacol ester
Dicarbonylacetylacetonato rhodiuM(I)
5-BroMo-2,1,3-benzothiadiazole, 97%
5-Hydroxyindole-3-acetic Acid
Methyl p-Anisate
Bis(ethylcyclopentadienyl)iron
7-bromo-2,4-dichloroquinazoline
4-(2-HYDROXY-PROPYL)--1-CARBOXYLIC ACID TERT-BUTYL ESTER
N6-Benzoyl-2'-O-methyl-adenosine
Eosinophil-Rnase A2 ELISA Kit
P-gp ELISA Kit
Human PRM3 ELISA Kit
Human DHFR ELISA Kit
IP3 ELISA Kit
Rat β-CTx (Beta Crosslaps) ELISA Kit
Chicken Immunoglobulin G,IgG ELISA Kit
PCI ELISA Kit
Human 50% complement hemolysis,CH50 ELISA Kit
FEP ELISA Kit
Purple Broth Base
Power-nitrate Medium
AluMinuM 2,4-pentanedionate, 99.995+% (Metals basis)
ZirconiuM Fluoride
5-(Methylsulfonyl)pyridine-3-boronic acid, 98%
2-DiMethylaMinopyridine
2-methyltricyclo[3.3.1.1~3,7~]decane
Trilostane
Dowex 50W-X4
pentacosanoic acid
INDOXYL ACETATE
1-ETHYL-1-METHYLPYRROLIDINIUM TETRAFLUOROBORATE
4-BroMothiophene-2-carboxaldehyde
ScandiuM(III) bis(trifluoroMethylsulfonyl)iMide
Dithizone, 98%
2,4-Difluoro-5-Methylbenzoic acid
4-Hydroxy-2-oxopyrrolidine-N-acetaMide
Methyl 4-chloro-8-Methylquinoline-2-carboxylate
3-Fluoro-4-Methylphenylboronic acid
4-(2-BroMophenyl)-2-Methyl-1-butene
2-Acetyl-1,3-cyclohexanedione
Tris(2,2,6,6-tetramethyl-3,5-heptanedionato)aluminum, 99% (99.9%-Al) [Al(TMHD)3]
5-Chloro-2-(methylthio)benzothiazole
4'-Fluoro-2'-(trifluoromethyl)acetophenone 98%
3-Methylhexyl Acetate