NHEK细胞株|人表皮角质形成细胞系技术优势大 货号:GD-C9918311
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
如何进行细胞复苏:1)从液氮容器中取出冻存管,直接浸入37℃温水中,并不时摇动令其尽快融化;2)从37℃水浴中取出冻存管,打开盖子,用吸管吸出细胞悬液,加到离心管并滴加10倍以上培养液,混匀;3)离心,1000rpm,5min;4)弃去上清液,加入含10%小牛血清培养液重悬细胞,计数,调整细胞密度,接种培养瓶,37℃培养箱静置培养;5)次日更换一次培养液,继续培养。【温馨提醒】1)从增殖期到形成致密的单层细胞以前的培养细胞都可以用于冻存,但Z好为对数生长期细胞。在冻存前一天Z好换一次培养液;2)将冻存管放入液氮容器或从中取出时,要做好防护工作(戴棉手套),以免冻伤;3)冻存和复苏Z好用新配制的培养液。细胞复苏的时候,有些初次实验员将冻存管从液氮中取出后,放在室温下,经常发生冻存管爆炸,该怎么避免出现这种情况吗?其中在拧紧冻存管的时候是否有哪些细节要注意的?一般常用的方法是取出冻存管后在超净工作台中用酒精棉球擦试管口,再稍拧松盖子,再放37度水浴锅中摇溶,在将细胞转移到装有37度预热过的培养基的试管中,迅速离心,再用培养基洗一遍。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Benzo[b]furan-2,5-diboronic acid, 98%
2-Thiopheneethanol
2-(TrifluoroMethyl)benzyl alcohol
Methyl 3-(3-boronobenzaMido)propionate
Indan-1,2-dione
3-Methyl-1-(4-sulfophenyl)-2-pyrazolin-5-one , 97%
2-BroMo-4-Methylbiphenyl-3-carboxylic acid
3-Fluoro-4-methoxybenzoic Acid
3,5-Dimethyl-4-methoxyaniline 99+%
2-Amino-5-nitrobenzonitrile
5(6)-Carboxyeosin diacetate
m-Tolyl isocyanate
Naftopidil dihydrochloride
METHYLSULFONYLACETONITRILE
Indole-5-carboxylic acid
TriethylaMine hydrobroMide
2',5'-DiMethoxyacetanilide
5-BroMo-2-Methoxyphenyl Mesylate
PotassiuM broMide, 99+%, for analysis
FUMARIC ACID
(-)-Epicatechin Gallate
Terephthalic dihydrazide
Maleicacidmonomethylester
di-2-naphthylamine
1,2-Diaminopropane
4-Chlorophenylboronic acid
2,7-Diaminofluorene
5-BroMo-2-Carboxy-3-Methylpyridine
3-AMinocouMarin, 97%
2-Butynyl p-toluenesulfonate, 98%
NiobiuM chloride
2′-O-Methylcytidine
4-[4-(1-Hydroxyethyl)-2-Methoxy-5-nitrophenoxy]butanoic Acid
YtterbiuM(III) acetate tetrahydrate
2-[4-(TERT-BUTYLDIMETHYLSILYLOXYMETHYL)-2-NITROPHENOXY]ACETIC ACID
3,5-Bis(trifluoroMethyl)benzyl alcohol
Trifluoromethylmaleic anhydride
Aluminum fluoride, anhydrous, 99+%
5-(4-Fluorophenyl)-3H-thieno[2,3-d]pyrimidin-4-one
Tri-n-butylgermane
3-Heptanone
4-Ethoxy-3-nitropyridine hydrochloride
Ethyl 1-methyl-4-piperidinecarboxylate
4-Hydroxy-azo-benzene;4-Phenylazophenol;Solvent yellow 7;C.I. 11800
3-Butyn-2-one
1-ForMylpiperidine
alpha-Octithiophene
5,8-DibroMoisoquinoline
3-AMinoMethyltetrahydrofuran, 97%
N-Boc-N'-Cbz-L-lysine
7-Hydroxy-5-Methyl-1,3,4-triazaindolizine
Butyrylacetic acid methyl ester
6-Hydroxy-3,4-dihydro-2-quinolinone
Tributylsulfoniumiodide
Acid Yellow 3
3-(4-CHLORO-PHENOXY)-PIPERIDINE-1-CARBOXYLIC ACID TERT-BUTYL ESTER
Methyl 5-p-tolylpyrazole-4-carboxylate
Tributyl borate
6-Methylquinoline, 98%
10z-Hymenialdisine
3-Boc-aminomethyl-azetidine
Tris(2,2,6,6-tetramethyl-3,5-heptanedionato)neodymium(III), 99% (99.9%-Nd) (REO) [Nd(TMHD)3]
3-Fluoro-2-nitroaniline 98%
Benzocaine
VALDECOXIB
5-METHYLTRYPTAMINE HYDROCHLORIDE
Propionyl chloride;Propanoyl chloride;
1-[4-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)benzyl]piperidine
(±)-2-Methyl-1-butanol
RutheniuM(II) Nitrosyl Chloride Monohydrate
1,4-Dicyanobenzene, 98%
Methyl 2-isothiocyanatobenzoate, 97%
Urethane
FuMaraldehyde Mono(diMethyl Acetal) (stabilized with HQ)
3-Hydroxy-1-adamantanecarbonitrile
methyl 2-mercapto-1-methyl-1H-imidazole-5-carboxylate
3-(2,8,9-trioxa-5-aza-1-silabicyclo[3.3.3]undec-1-yl)propyl 2-methylprop-2-enoate
Diclofenac
AP-1 ELISA Kit
Mouse Complement 4,C4 ELISA Kit
Human CTSB ELISA Kit
Human GR ELISA Kit
17-ks ELISA Kit
Hamster matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA K
SERT ELISA Kit
Human GALK1 ELISA Kit
CoX-4 ELISA Kit
GCSFR ELISA Kit
Modified Plate Count Agar
White Medium
CESIUM DICHROMATE
Glycidyl Phenyl Ether
5-BroMo-2-(4-Morpholinyl)benzonitrile, 98%
2-Hydroxybenzyl alcohol, 99%
Terephthalic dihydrazide
o-Chloro Thiophenol
3,4-Diethoxybenzoic acid
2-Chlorobenzotrifluoride
N-BROMOPHTHALIMIDE
FLUOXETINE HYDROCHLORIDE
2,2,2-TrichloroacetaMide
5-BroMo-3-nitrosalicylaldehyde, 97%
4'-Fluorobiphenyl-4-carboxylic acid, 96%
3-(TrifluoroMethoxy)phenylacetic acid
Pyraclostrobin
3-Fluoro-5-trifluoroMethylphenylboronic acid
2,3,5-TRIFLUOROBENZOIC ACID
4-Pentyloxybenzoic acid
4-AMinoveratrole
4-Methylphenylsulphur pentafluoride
2,3-O-Isopropylidene-β-D-ribofuranosylamine p-toluenesulphonate salt ,98%
2-Cyanophenylboronic acid,1,3-propanediol
3-Benzylamino-1-propanol