DH-82细胞株|狗肾恶性组织细胞系折扣力度大 货号:GD-C9820584
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Fluoro-4-nitrobenzeneboronic acid pinacol ester, 96%
2,2,3,4,4,5-Hexachlorobiphenyl Standard
4'-NITRO-4-DIMETHYLAMINOAZOBENZENE
4,7-DIBROMO-2,1,3-BENZOTHIADIAZOLE
2,3-Dichloropyridine-4-boronic acid, 95%
4-SULFOPHTHALIC ACID
3-CHLOROPHENYL ISOTHIOCYANATE
1-Cyclohexyl-1-pentanol
3-Amino-4,4,4-trifluorobutyric acid
3-Amino-3,4-dihydroquinolin-2(1H)-one hydrochloride
2-Vinyl-4-quinazolinol
Barium molybdate
AMMONIUM CERIUM(IV) SULFATE
5-Bromo-2-chlorobenzoic acid
5-Chloropyridine-2-boronic acid
2-AMino-5-nitrothiazole
(R,R)-N,N'-Bis(trifluoroMethanesulfonyl)-1,2-diphenylethylenediaMine
1-(3-broMopyridin-2-yl)ethanone
Succinyl chloride, ca. 95%, AcroSeal
MES, Anhydrous, Biological Buffer
(CyanoMethyl)triphenylphosphoniuM Chloride
betulinic acid
N-Aminomorpholine
1-Bromoheptane
octyl butyrate
Palladium bromide
3,5-Di-tert-butyl-4-hydroxybenzyl alcohol
2-Amino-5-fluorobenzoic acid methyl ester
4-(4-Hydroxy-3-Methoxyphenyl)-2-butanone, 97%
2-BroMobenzaMide, 98%
2-Chloro-6-fluoro-3-Methylbenzonitrile, 97%
Triazoxid
Bis(2,2,2-trifluoroethyl) Phosphite , 94%
tert-Butyl (4R,6R)-2-[[[6-(2-4-fluorophenyl)-5-isopropyl-3-phenyl-4-(phenylcarbaMoyl)pyrrol-1-yl]ethyl]-2,2-diMethyl-1,3-dioxan-4-yl]acetate
MALTOPENTAOSE
3-BroMo-7-chloroiMidazo[1,2-a]pyridine
(1-Benzyl-1H-imidazol-2-yl)methanol , 95%
(S)-(-)-1-Phenylethylamine ,99% [ee:98%]
4'-Hydroxy-2'-methylacetophenone, 97%
4-Ethyl-2-methoxyphenol, 98%
7-Fluorotryptamine
2-Chloro-4-fluorobenzoic acid
1-AMINOHEPTANE
2-(Cyanomethylthio)acetic acid
3,5-Bis(trifluoroMethyl)benzyl broMide
3-Methylbenzyl chloride
3,5-Dichloro-2,6-diMethoxybenzoic Acid
4-BroMo-2-(trifluoroMethoxy)benzaldehyde
1,3-DiMethyl-1H-pyrazole-5-carboxylic acid, 97%
Toluidine Blue O
1-Chloro-2,2-diMethylpropane
4-(Trifluoromethyl)acetophenone
5-norbornene-2,2-dimethanol
guaiacol sulfonic acid potassium
3-Ethylpyridine
2-Isopropylphenyl isocyanate
9,9-DiMethyl-9H-fluorene
CYCLOOCTYL ISOTHIOCYANATE
(3-BENZYLOXY-PHENYL)-ACETIC ACID
Triethyl 4-phosphonobutyrate
1,2,4-Tribromobenzene
Fmoc-5-aminopentanoic acid
D-Proline Benzyl Ester Hydrochloride
4-[5-(TRIFLUOROMETHYL)PYRID-2-YLOXY]THIOBENZAMIDE
3-Thiophenezoic acid
Phenylhydrazine
L-Lysine dihydrochloride 99%
Diisopropyl?D-(-)-Tartrate
1-(4-BroMophenyl)iMidazole
(5-ForMylpyridin-3-yl)boronic acid
Diphenylphosphoryl azide, 98%
(R)-(+)-1-Phenyl-1-propanol, 95%, 98% ee
TetrabutylaMMoniuM Hydrogen Sulfate [Reagent for Ion-Pair ChroMatography]
TetrabroMophthalic Anhydride
3,6-Diacetyl-9-ethyl-9H-carbazole
3-(methylsulfonyl)pyrrolidine hydrochloride
N-BOC-INDOLINE-7-CARBOXYLIC ACID
1-(4'-Bromophenoxy)-1-ethoxyethane
Porcine oxidized lowdensity lipoprotein,OxLDL ELISA Kit
IL-2 ELISA Kit
Human Tumor necrosis factor α,TNF-α ELISA Kit
Human GK2 ELISA Kit
β2M ELISA Kit
CD40L ELISA Kit
IL-6 ELISA Kit
BrucellaAbIgG ELISA Kit
hFABP ELISA Kit
KIM1 ELISA Kit
Brilliant Green Agar Modified
BCP Medium with 0.2% Soluble Starch
3-(o-Methoxyphenoxy)-1,2-Propanediol
YtterbiuM(III) perchlorate, 50% w/w aq. soln., Reagent Grade
5-Sulfosalicylic acid dihydrate
2-METHYL-2-IMIDAZOLINE
betulinic acid
Graphite
Aescine
2,2'-methylenebis(6-tert-butyl-4-methyl-phenol)
5-Methyl-2-acetylfuran
(R)-(+)-2-AMINO-1-FLUORO-3-METHYL-1,1-DIPHENYLBUTANE
5-Chloro-2-Iodopyridine
Zinc MolybdenuM oxide, 98%
1-[2-(4-Morpholinyl)ethyl], 97%
2,3-Diethyl-5-Methylpyrazine
2-Methylpyridine-4-carboxylic acid
1-(5-BroMo-2-Methoxy-phenyl)adaMantane
Isobutyric anhydride
9H-Fluorene-9,9-diMethanol
4-ISOPROPYLBENZONITRILE
Hexafluoroglutaric Acid
Ethylene Glycol Mono-m-tolyl Ether
5-Bromo-2-methoxyaniline
(1S,2S)-(+)-1,2-Cyclohexanediamino-N,N'-bis(3,5-di-t-butylsalicylidene)cobalt(III) p-toluenesulfonate monohydrate