NCI-H727[H727]细胞株|人肺支气管良性肿瘤细胞系折扣力度大 货号:GD-C9820392
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-BroMo-2-nitrobenzoic acid, 97%
5-BroMovanillin
5-MethoxytryptaMine hydrochloride
3-(N-PropylaMinocarbonyl)phenylboronic acid
3-Picoline-N-oxide
4-BroMophenylacetic acid
ETHYL 5-METHYLISOXAZOLE-3-CARBOXYLATE
Benzoyl isocyanate
2-Fluoro-3-(trifluoromethyl)benzeneboronic acid 97%
4-Methyl-2-pyridyl Trifluoromethanesulfonate
3-Ethoxy-5-fluorobenzeneboronic acid 95%
2-(4-FLUOROBENZYLSULFONYL)ACETAMIDOXIME
2-METHYL-6-VINYLPYRIDINE
5-AMINO-1-PHENYLPYRAZOLE-4-CARBONITRILE
Diethyl phenylMalonate
1,2,4-1H-Triazole
4-(N,N-DiMethylaMinosulfonyl)-7-aMino-2,1,3-benzoxadiazole
Methyl 3-broMo-5-ethoxybenzoate
YtterbiuM(III) chloride, 99.9%, (trace Metal basis), anhydrous
SodiuM Gluconate, USP
2-Fluoro-4-iodoaniline
Desonide
4-Hydroxy-3-nitroacetophenone
4-Benzyloxy-1-nitrobenzene
2-Fluoro-5-nitropyridine
N-Boc-L-Phenylalaninol
4-BUTYLBENZONITRILE
Iron(III) sulfate, Puratronic|r, 99.998% (Metals basis)
n-Decyl Methyl sulfide, 97%
4-tert-Butylcalix[8]arene, 96%
(S,S)-(-)-Bis(α-Methylbenzyl)aMine Hydrochloride
2-AMino-5-nitropyridine
2,3,5,6-TETRAFLUORO-4-AMINOBENZOTRIFLUORIDE
OxaMide
TRIPHENYLSULFONIUM TETRAFLUOROBORATE
Quinacridone
1-Fluoro-1,2,2-trichloroethane (FC-131) 97%
4-Bromo-5-methylthiophene-2-carboxadehyde, 95%
2-(3-chlorophenoxy)aniline(SALTDATA: HCl)
2'-Chloro-[1,1'-biphenyl]-4-carboxaldehyde
Acetyl benzoyl peroxide
4-(TRIFLUOROMETHYLSULFONYL)ANILINE
3'-CHLORO-2',6'-DIFLUOROACETOPHENONE
FluoroMetholone
trans-3-MethylcinnaMic acid
L-Alanine Isopropyl Ester Hydrochloride
2-Hydroxy-4-Methylbenzonitrile
4-Methyl-2-nitrophenol, 99%
5-BroMo-2,4-diMethyl-1,3-thiazole, 97%
2,4,5-TRIHYDROXYBUTYROPHENONE
2,2',4,4'-Tetrahydroxydiphenyl Sulfide [Coupler for Reprography]
trimethylfluorosilane
3,4-Difluorophenylboronic acid
Methanone,(4-methoxyphenyl)-4-piperidinyl-
Phenylsulfinylaceticacidethylester
4-Fluoro-3-(trifluoromethyl)benzonitrile
4-(4-Fluorophenoxy)benzoic acid
3-Ethylphenol
TITANIUM HYDRIDE
2-Aminobenzotrifluoride
Hexadecane ,98.5%
4-Chloro-2,5-difluorobenzaldehyde
2,4-Dibromo-6-fluorobenzaldehyde
Ethyl ziram
4-Benzylpyridine
1,3-Dibromo-5,5-dimethylhydantoin
Methyl 2-chloropropionate
Methyl trifluoroMethanesulfonate
1-BroMo-3,4-dichlorobutane
6-Carboxy-2-fluoropyridine-3-boronic acid
Aniline, 99.5%, extra pure
(R)-(-)-1-Benzyl-3-aMinopyrrolidine, 99%
SodiuM 1-Propanesulfonate
4,4'-DibroMotriphenylaMine
2,4-Pentanediol
bromo-[(3,4-difluorophenyl)methyl]zinc
Tetramethylbenzenedisulfonyl dichloride
Benzyl 7-oxa-3-azabicyclo[4.1.0]heptane-3-carboxylate
Porcine myeloperoxidase,MPO ELISA Kit
S100B ELISA Kit
Human TAP ELISA Kit
Human Ub ELISA Kit
IFN-β/IFNB ELISA Kit
αHSP ELISA Kit
Chicken basic fibroblast growth factor,bFGF ELISA Kit
Human BLVRA ELISA Kit
GMP-140 ELISA Kit
GC ELISA Kit
Alkaline Peptone Water
Soybean-Casein Digest Agar Medium with Lecithin
AcetohydroxaMic acid
TERBIUM(III) CHLORIDE HYDRATE
Methyl 5,6-Dichloronicotinate
2-Fluoro-4-Methylpyridine
Desonide
1-(2-Bromoethoxy)-4-nitrobenzene
(R)-(-)-1-Aminoindan
Hydrocinnamic acid
Methyl DL-mandelate
Nickel chloride
2,3,4-Trifluorobenzaldehyde
(S)-2-(Boc-aMino)-4-(FMoc-aMino)butyric acid, 95%
3-Nitro-o-phenylenediaMine, 98%
1-(3-Fluorophenyl)hoMo Monohydrochloride, 98%
2-Chloro-5-Methylpyrazine
HEXABUTYLDITIN
2-(Methoxycarbonyl)ethylboronic acid, pinacol ester
Phlorizin
Ethyl Nonafluorobutyl Ether (Mixture of isoMers)
Allylthiourea
Acetic acid, ammonium salt, for analysis ACS, 97+%
3,4-Dimethoxyphenol
2-(tert-Butylamino)ethanol, 99+%