L1210细胞株|小鼠白血病细胞系技术优势大 货号:GD-C9919107
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Bicinchoninic acid disodiuM salt
4-(TrifluoroMethyl)benzylaMine
Diacetyl hydrazine
2-BroMothioanisole
2-Chloro-3-(trifluoroMethyl)phenylacetic acid, 97%
2'-Methylacetophenone
4-METHOXY-3,5-DINITROTOLUENE
Silver (I) oxide, 99+% (99.99%-Ag) PURATREM
4-Chloro-2,5-dimethoxynitrobenzene
Tetraphenylphosphonium tetrafluoroborate, 98+%
(S)-2-Benzyl-aziridine
3-Methylphenyl-L-alanine
2-CHLORO-5-FLUOROBENZOYL CHLORIDE
3-Amino-3-phenylpropionic acid
N,N-DiMethyl-1,4-phenylenediaMine
1-Methyl-1H-pyrazole-3-carboxylic acid
1,8-DibroMohexadecafluorooctane
1,10-Undecadiene
Triphenyl phosphite, 99%
Riboflavin 5'-Phosphate SodiuM, USP
4-Methyloctane
trans-Cinnamic acid
testosterone decanoate
Benzyl phenyl ether
2,3,4,5,6-pentafluoroanisole
Cyclobutyl bromide
Cellulose CM
PotassiuM tetrachloropalladate(II), PreMion|r, 99.99% (Metals basis), Pd 32.2% Min
2-(Phenylsulfonyl)thiophene, 98%
4-(HydroxyMethyl)phenoxyacetic acid, 98+%
BroMacil
Sucrose Monolaurate
1-BOC-6-Methoxyindole-2-boronic acid
Phosphonitrilic chloride triMer
Methyl (2S,3R)-(?)-2,3-dihydroxy-3-phenylpropionate
1-BENZOYL
1-(4-Fluorophenyl)-5-methyl-1H-pyrazole-4-carboxaldehyde
2,4,5-Trifluorobenzoyl chloride 99%
4-benzyl-3,5-dimethyl-1H-pyrazole(SALTDATA: FREE)
3-Chloro-4-(ethoxycarbonyl)benzeneboronic acid, pinacol ester 96%
Lead bromate
2-FLUORO-6-(4-CHLOROPHENYLTHIO)BENZONITRILE
Cyclophosphamide
ErythroMycin;E-Mycin;Eritrocina;ErythroMid;Erycen;Erycin;ErycinuM;ErMysin
2,1-Benzisoxazole-3-carboxylic acid
AMiodarone Hydrochloride
5-(Benzyloxy)-2-fluorophenylboronic acid
4-MethoxybenzylaMine, 98%
3-Cyano-1-propylboronic acid pinacol ester, 97%
1,6-HEXANEDIAMINE
N-ForMyl-DL-ethionine
1-Bromo-4-(trifluoromethoxy)benzene
Fmoc-Lys(Mtt)-OH
tert-butyl 3-(aminomethyl)benzoate
2,5-Hexanedione, 3-mercapto-
Carbachol
4-Ethoxybenzaldehyde
2-(Cyclohexyloxy)ethanol
SucciniMidyl 4-[3,5-DiMethyl-4-(4-nitrobenzyloxy)phenyl]-4-oxobutyrate , 96%
Cadmium sulfide, 98%
2-[4-(Methoxycarbonyl)-5-methyl-2-oxo-2,3-dihydro-1H-pyrrol-3-yl]acetic acid, 97%
3-Isopropylisoxazol-5-amine ,97%
Magnesium iodate tetrahydrate, pure, 98%
2,6-Dichloro-3-(trifluoromethyl)pyridine
Aluminum phosphide
N-(2-Hydroxynaphthoyl)-2,4-dimethoxy-5-chloroanilide
Diallyldisulfide
2-Chlorophenylboronic acid (contains varying aMounts of Anhydride)
1-Acetyl-1H-1,2,3-triazolo[4,5-b]pyridine
5-Chloro-2-fluoronicotinaldehyde
3-Nitrobenzenesulfonyl chloride, 97%
(3-Piperidinophenyl)MethylaMine, 95%
3,4-Diaminobenzoic acid
1,2-Naphthalenedicarbonitrile
2,4,4-Trimethyl-1-pentene
2-DEOXY-N-PHENYLGLUCOSYLAMINE
cis-3-Hexenyl salicylate
Basic Yellow 29
VC ELISA Kit
α1AGP ELISA Kit
Human REG3α ELISA Kit
Human non-neuronal enolase,NNE ELISA Kit
SOD ELISA Kit
TLR3 ELISA Kit
CFB ELISA Kit
Human colon cancer-specific antigen-2,CCSA-2 ELISA Kit
Human anti-Endostatin antibody,ES-Ab ELISA Kit
VEGFR-1/Flt1 ELISA Kit
RNS Medium
Anaerobe Broth MIC
DiMethyl isopropenyl phosphate, 95%
Benzyl Glycinate Hydrochloride
5-Chloro-2-fluoropyridine-3-boronic acid, 97%
2-Fluoro-6-nitrobenzoic acid, 98%
trans-Cinnamic acid
Vat Brown 57
Ferritin
2-(trifluoromethyl)styrene
4,4'-Difluorobenzhydryl
TRIS(6,6,7,7,8,8,8-HEPTAFLUORO-2,2-DIMETHYL-3,5-OCTANEDIONATO)YTTERBIUM
4-Tert-Butylpheno
4-BroMo-2-fluoro-1-(n-propoxy)benzene, 99%
5-Benzoylpentanoic acid, 99%
Etridiazole
IMidacloprid
PicraMic Acid (wetted with ca. 25% Water, containing 25g on a dry weight basis), 98%
Z-L-Prolinol
Purine
5-Chloro-2-isopropoxypyridine-3-boronic acid
Ammonium sulfide, 20% solution in water
2-Ethoxythiazole
2,2-Difluoro-1,3-benzodioxole-4-boronic acid
6-Chloropyridine-3,4-dicarboxylic acid