MV-1-Lu细胞株|鼬肺上皮细胞系折扣力度大 货号:GD-C9819203
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3'-Chloroacetophenone, 98+%
(R)-(-)-1-Methyl-3-pyrrolidinol
SodiuM acetate-1-13C
2-HYDROXY-4-METHYLQUINOLINE
Fluoxetine hydrochloride
8-Anilino-1-naphthalenesulfonic acid MagnesiuM salt
Lauryl chloroforMate
(3-Piperidinophenyl)methylamine , 95%
2-(1,3-Benzodioxol-5-yl)-6-chloroimidazo[1,2-b]pyridazine
4,5,6,7-Tetrahydro-3-(trifluoromethyl)-1H-indazole
4-Benzoyl benzylamine hydrochloride
4-Pyridineethanol
3,5-BIS(METHYLSULFONYL)ANILINE
alpha,alpha,alpha-Trifluoro-o-toluoyl chloride
2-Cyano-5-Methylbenzenesulfonyl chloride
FlufenaMic acid
2,4-DiMethyloxazole
N-Methyl 4-broMobenzenesulfonaMide
VancoMycin hydrochloride, >900 MicrograM/Mg
SodiuM Pyrophosphate, Decahydrate, Crystal, Reagent, ACS
Hydroquinidine Hydrochloride
Ethyl Azidoacetate
Brilliant Blue G
8-Fluoroisoquinoline
Alpha,Alpha,Alpha-Trifluoro-p-tolunitrile
Decanoyl chloride
3-Bromobenzenesulfonyl chloride
4-Fluoro-3-Hydroxybenzonitrile
Dicarbonylcyclopentadienylcobalt
AdaMantane-1,3-dicarboxylic acid, 98%
3-AMinobenzenesulfonaMide, tech. 80%
4-PiperidineMethanol
1,1,2,3,3-PENTACHLOROPROPANE
PENTACHLOROTHIOPHENOL
2,4-DIMETHYL-6-NITROPHENOL
1-BroMo-4-(MethoxyMethoxy)benzene
(R)-(+)-(3-Chlorophenyl)oxirane
Ethyldiphenylphosphine, 99%
2,2'-Dihydroxydiphenylmethane
(+/-)-Limonene
(Chloromethyl)dimethylvinylsilane(Vinyl(chloromethyl)dimethylsilane)
Tetraphenylmethane
N-Methyl-L-prolinol
3-FLUORO-L-PHENYLALANINE
PotassiuM pyrosulfate
Benzanilide
3,5-Dichloro-2,4-difluoroaniline
Undecafluorohexanoyl Fluoride
Tris(4-broMophenyl)aMiniuM hexachloroantiMonate, 95%
SeleniuM Sulfide, USP
Diisodecyl 4-Cyclohexene-1,2-dicarboxylate
N-propylaniline
Pigment Red 2
ethambutol dihydrochloride
2-Fluoronicotinic acid
TETRAPROPYLAMMONIUM PERCHLORATE
sec-ButylaMine
5-BroMo-2-benzoxazolinone
2-Methylfuran-3-boronic acid, pinacol ester
5-Fluorouracil
3-Iodoisopropylbenzene
4-Chloro-2,3-dimethylpyridine-N-oxide ,98%
Methyl 4'-nitro[1,1'-biphenyl]-4-carboxylate
2-(4-NITROPHENOXY)ETHANOL
2-Norbornanemethanol
3-CHLORO-2-FLUORONITROBENZENE
CalciuM chloride fused
DI-TERT-BUTYL PHOSPHITE
1-BroMo-2,3-dichlorobenzene
4-MethoxycinnaMonitrile (cis- and trans- Mixture)
Tetrahydrothiophene-3-carboxylic acid 1,1-dioxide
2,6-Difluorobenzaldehyde, 98%
Tungstic acid
3-Pyridylacetic Acid Hydrochloride
ethylenediaminetetraacetic acid*trisodium
Glutaraldehyde sodium bisulfite addition compound
Dextrin
1-chloro-3-ethoxy-2-propanol
PPIL1 ELISA Kit
Smad7 ELISA Kit
Human Progesterone,PROG ELISA Kit
E2/UBCE ELISA Kit
IL13 ELISA Kit
A-GST ELISA Kit
Chicken Myoglobin,MYO/MB ELISA Kit
CH ELISA Kit
Human hemoglobin-advanced glycosylation end products,Hb-AGE ELISA Kit
TAP ELISA Kit
Modified Plate Count Agar
Letheen Broth Base
Ethoxyamine hydrochloride
FluoroMethyl 1,1,1,3,3,3-hexafluoroisopropyl ether, 98+%
5,7-Dichloro-4-Hydroxy-2-(TrifluoroMethyl)Quinoline
2-Fluoro-4-nitrobenzoic acid, 98%
Ethyl Azidoacetate
4-Aminoanisole-3-sulfonic acid
4-nitrophenyl-alpha-L-fucopyranoside
3,5-Dimethylbenzoic acid
SODIUM 4-N-OCTYLBENZENESULFONATE
N-(ISOBUTOXYMETHYL)ACRYLAMIDE
3-Hydroxy-5-Methylisoxazole, 97%
2-(4-Methylphenyl)-4-phenylthiazole, 97%
SodiuM Metasilicate, anhydrous, tech.
tert-Butyl isocyanate
6-Methylnicotinic Acid Methyl Ester
N-Methoxy-N-Methyl 4-borono-2-fluorobenzaMide
2-Chloro-6-trifluoroMethylnicotinic acid
3-CHLORO-4-FLUOROPHENYL ISOCYANATE
1-(4-METHOXY-PHENYL)-2-METHYLAMINO-ETHANOL
Benzyl-propyl-amine
Lead citrate, trihydrate, 96%
7-Methylindole-3-carboxaldehyde 98%
1,2,3,4-Tetrahydrocarbazol-4-one