T/GHA-VSMC细胞株|人血管平滑肌细胞系折扣力度大 货号:GD-C9819182
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Benzeneboronic acid, 98+%
Dodecyl glucopyranoside
PalladiuM(II)[1,3-bis(diphenylphosphino)propane]-bis(benzonitrile)-bis-tetrafluoroborate
2-BroMo-6-(trifluoroMethoxy)benzothiazole
SodiuM Myristate , 98%
5-NitrobenziMidazole
Methyl 3-(broMoMethyl)benzoate
tert-Butyl 4-(aminocarbothioyl)tetrahydropyridine-1(2H)-carboxylate , 95%
2-Bromo-4-methylaniline hydrochloride
3,5-Dichloro-4-fluoroaniline
Isopropyl Crotonate
3-(PERFLUOROOCTYL)-2-HYDROXYPROPYL ACRYLATE
Chloroacetaldehyde
2-Amino-5-fluorobenzonitrile
5-BroMo-2-fluorobenzonitrile
DiMethyl chloroacetal
(1S,2S)-N,N'-Dihydroxy-N,N'-bis(3,3,3-triphenylpropionyl)cyclohexane-1,2-diaMine
Boron Trifluoride - Butanol Reagent (10-20%) [for Esterification] (1ML*10)
Uracil, 99+%
SodiuM Metabisulfite, Granular, NF, EP, BP, JP
2,4-Bis(alpha,alpha-diMethylbenzyl)phenol
1-Iodo-4-nitrobenzene
2-Fluoropropionic acid
p-Methyl Thiophenol
4-methylcatechol
Phenylacetyl disulfide
2-BroMo-5-Nitrobenzoic Acid Methyl Ester
n-Butyltin trichloride, 96%
N-Benzyloxycarbonyl-D-Methionine, 98%
BariuM iodate Monohydrate
2-AcetaMido-3-broMo-5-nitropyridine
2,2'-THENIL
PERFLUORO(METHYLCYCLOHEXANE)
LITHIUM IODIDE
N-HEPTADECANONITRILE
7H-Pyrrolo[2,3-d]pyrimidin-4-amine sulfuric acid
3-Formyl-1H-indole-7-carboxylic acid, 95%
2-[2-(5-Methylfuran-2-yl)vinyl]-4,6-bis(trichloromethyl)-1,3,5-triazine
5-Methyl-3-heptanol
2,4,6-Tri-tert-butylphenol
Tetrakis(triphenylphosphine)palladium
1,6-HEPTADIYNE
2-METHYLVALERIC ACID
7-HydroxycouMarin
4-Cyclohexylphenol
3-(MethoxyMethoxy)phenylboronic acid
Ethyl broMopyruvate, 80-85%
2-(2-Pyrazinyl)Malondialdehyde, 95%
7-Tridecanone
N,N'-Diacetylhydrazine
2-Methyl-5-Nitrobenzoic acid
2-Methyl-2-butanol
3-Tert-Butyl-Benzonitrile
Morpholine-4-carboximidamide hydroiodide
4-Fluoromandelic acid
3-BroMophenylhydrazine hydrochloride
LithiuM tri-tert-butoxyaluMinuM hydride
3-NITROPHENETOLE
Lumogallion [Fluorimetric reagent for Al, Ga and other metals]
2-(4-Bromobutyl)-1,3-dioxolane
2-(Methylthio)phenyl isocyanate 95+%
2-Naphthylacetonitrile, tech., 90-95%
Chloromethyl chlorosulfate
1-Bromo-5-hexanone
2,4-Diaminopyrimidine
2,2'-Biphenol
N-alpha-Benzoyl-DL-arginine-4-nitroanilide hydrochloride
1-Benzyl-3-aMinopyrrolidine
4-Butoxy-3,5-dichlorophenylboronic acid
Allyl Mercaptan, tech., 70%, reMainder Mainly sulfide
2-AMino-5-broMo-4-picoline, 97%
alpha-BroMo-gaMMa-butyrolactone
LevetiracetaM
lead(ii) thiocyanate
1H-Pyrazolo[3,4-b]pyridine-3-carboximidamide,1-[(2-fluorophenyl)methyl]-, monohydrochloride
2-Methyl-5-nitrobenzotrifluoride
3-(4-Fluorophenoxy)azetidine hydrochloride
GR-β ELISA Kit
TAF4 ELISA Kit
Apo-A1 ELISA Kit
Human aryl sulfatase A,ASA ELISA Kit
IL2 ELISA Kit
α2-AP ELISA Kit
PPAR-B ELISA Kit
Human MCP4 ELISA Kit
Human Hemoglobin C,HbC ELISA Kit
DSPP ELISA Kit
Soya Casein Digest Lecithin Polysorbate Agar Base
Trypticase Soy Broth
YttriuM ingot/button, ^=50.8MM (2.0in) dia x 11MM (0.43in) thick, 99% (REO)
Tin fluoroborate
GTP
5-Chloro-2-Fluorobenzonitirle
1-Iodo-4-nitrobenzene
4-Phenylbutylamine
2-Amino-4-Methoxylpyridine
Trifluoromethoxybenzene
2-(TRIMETHYLACETYL)THIOPHENE
Bis(diphenylphosphino)methane
4-BroMo-2-Methoxypyridine
Di-n-octyl phenylphosphonate, 97%
AntiMony(V) fluoride coMpound with graphite, 1:1 Mixture
SodiuM Methylate
FMoc-Tyr(tBu)-OH
Zinc chloride
3-(4-CYANO-PHENYL)-ACRYLIC ACID METHYL ESTER
Aniline Blue (spirit soluble)
Boc-3-(trifluoroMethyl)-D-phenylalanine
n-Propylpyrazine
Vinyl Hexanoate (stabilized with MEHQ)
Alizarin Cyanin Green F
Bis(cyclopentadienyl)chromium, min. 95%, sublimed (Chromocene)