PC-12未分化细胞株|大鼠肾上腺嗜铬细胞系优惠力度大 货号:GD-C9720550
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 1H-indazole-6-carboxylate, 95%
4-BroMo-3,5-diMethylisoxazole
4-AMinonaphthalene-1-sulfonic acid, 98%
2-Iodobenzyl broMide
2-BroMo-6-Methoxypyridine
3-Hydroxy-N-2-naphthyl-2-naphthaMide
2,4-Dichloro-1-(2-iodophenoxy)benzene , 95%+
4-(2-Oxo-benzooxazol-3-yl)-butyric acid ,97%
4'-Diethylaminoacetophenone
1,2-Dibromo-1,2-dichloroethane
4-Isobutylbenzoic Acid
7-HYDROXYFLAVANONE
BOC-L-3-Trifluoromethylphe
Mercurous oxide,black
TetraMethylaMMoniuM acetate
Alloxan Monohydrate
DorzolaMide Hydrochloride
4-Chloro-3-forMylbenzonitrile
Baccatine III, 95%
TrolaMine, NF
Hexyl Sulfide
2,2-Dimethylhexanoic acid
cytidine 5'-(disodium phosphate)
Sulphur Black 1 -Solubilised
4'-Chloroacetanilide
2-Fluorobenzaldehyde
4-N-HEXYLOXYBENZOYL CHLORIDE
5-AMinonicotinic Acid
2-BroMo-4-fluorotoluene, 98+%
TetraphenylarsoniuM chloride hydrate, 96%
3-AMinophenylacetic acid
Skatole
2-(2-Naphthoyl)-1-(M-toluoyl)hydrazine, 98%
N-Methyl 2-Methyl-6-MethoxyMethylaniline
AZELANITRILE
(S)-(-)-4-AMino-2-hydroxybutyric acid
4-Methyl-2-pentanone
N-Boc-4-piperidinecarboxaldehyde
4-Methyl-2-pyridin-3-yl-thiazole-5-carboxylic acid
Baccatine III, 95%
2-Ethylhexyl chloroformate
Perospirone hydrochloride
3-(BOC-AMINO)-1-PROPANOL
TRANS-STILBENE
4,4-DiMethoxy-2-butanone
Bis(4-Methoxyphenyl) disulfide
(S)-(+)-Ketopinic Acid
4-(3-BroMophenyl)Morpholine, HCl
N-Methylaniline, 99%
1,1,1-Trimethoxypentane
1-(2-Cyanoethyl)-2-MethyliMidazole
Solvent Blue 59
Naproxene
1-(furan-2-yl)but-3-en-1-ol
Cyclohexanecarboxylic acid chloride
SODIUM PERRHENATE
(+)-PotassiuM Ds-threo-isocitrate Monobasic
Propiolic acid
Triphenylsilanol, 98%
Sand, 40-100 mesh, for analysis
2-Methyl-4-[(methylamino)methyl]-1,3-thiazole 97%
Amlodipine besylate
3-hydrazino-1,2-benzisothiazole 1,1-dioxide(SALTDATA: FREE)
Butyl isobutyrate
1,3-BIS(2,4,6-TRIMETHYLPHENYL)-4,5-DIHYDROIMIDAZOLIUM TETRAFLUOROBORATE
PROPRANOLOL HYDROCHLORIDE
Iron oxide red;Iron(III) oxide;Ferric oxide
MagnesiuM nitrate hexahydrate
1H-IMidazole-4-carboxylic acid
Methyl Pentafluorobenzoate
(Triphenylphosphoranylidene)acetaldehyde, 97%
LithiuM aluMiniuM hydride bis(tetrahydrofuran), 1M solution in toluene, AcroSeal
3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate
Methyl (Triphenylphosphoranylidene)acetate
(S)-(+)-2-Benzylamino-1-phenylethanol
1,1'-Biphenyl, 4-ethyl-4'-pentyl-
N-alpha-t-BOC-o-benzyl-L-serine
lithium metaphosphate
NT3 ELISA Kit
CICP ELISA Kit
Human NLRP3/C1orf7/CIAS1/NALP3/PYPAF1 ELISA Kit
Human GALNT6 ELISA Kit
Monkey TARC (Thymus Activation Regulated Chemokine) ELISA Kit
POMC ELISA Kit
Chicken Vascular Endothelial cell Growth Factor,VEGF ELISA Kit
PKCi ELISA Kit
GP-ⅡbⅢa ELISA Kit
TNFaR ELISA Kit
Lactose Peptone Broth
Lactose Monohydrate Broth
Nonyl Acetate
Phenoxazine, 98%
4-n-Pentylcyclohexanone, 98+%
3-Hydroxy-2-iodopyridine, 98%
2,2-Dimethylhexanoic acid
N,N'-dibenzylethylenediamine
Disperse Orange 61
1,3-Acetonedicarboxylic acid
2-BROMO-4,5-DIMETHOXYBENZYL ALCOHOL
2-Aminonicotinic acid
Methyl acrylate
D-(-)-Salicin, 99%
4-Cyanobenzenesulfonyl chloride, 97%
DiMethylchlorosilane
1-Hexyne
HYDROGEN TETRACHLOROAURATE(III)
1-Tetralone
N,N-Diisopropyl-4H-benzo[d][1,3,2]dioxaborinin-2-aMine
1,2-Hexadecanediol , 98%
4,7-Dibromo-2,1,3-benzothiadiazole
3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)-benzoic acid
Methyl 4-(2,4-dichlorophenoxy)butanoate
(R)-(+)-N,alpha-Dimethylbenzylamine, 99+%