MEF(CF-1)细胞株|小鼠胚胎成纤维细胞系优惠力度大 货号:GD-C9720027
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明:请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式:T25培养瓶(一般是贴壁细胞使用,悬浮细胞也可以)、离心管(悬浮细胞使用多,当然,有些公司也会用这种离心管形式发贴壁细胞,但是,长期经验来说,贴壁细胞容易发生形态变化,估计是细胞培养空间狭小,血清不足导致(Z主要是怕运输延误导致这些情况发生),个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用,主要是冬天天气冷,温度低于4℃的地方,都要考虑冻存细胞)。T25培养瓶:是用T25细胞培养瓶直接发货的形式。收到细胞后,拆开包装T25培养瓶的自封袋,不拆封口膜,表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上,再处理细胞。首先将T25中培养基取出装好备用,如细胞密度高于80%,可以直接消化传代,相反则加入5-6ml培养基放回培养箱继续培养即可。离心管:是用15ml离心管发货的形式,只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上,再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶,可以暂时T75培养瓶,加入10-15ml培养基,建议10ml培养基,也可以使用T175培养瓶,加入50-60ml培养基,培养瓶越大,需要的培养基越多。当然如果刚接收到细胞密度不够的话,Z好不用大瓶子,先用小瓶子养起来再换大的,不然会长得很慢,严重的,会导致细胞死亡等危险),平衡完成后,离心(1000rpm,3min)收集细胞,弃上清,用新的培养基重悬细胞并接种至培养瓶或皿中,放回培养箱继续培养。干冰:是用本公司冻存液冻存细胞后,直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-BroMo-N-cyclopropylbenzenesulfonaMide, 97%
Tributyl borate
CHLOROMETHYL METHYL SULFIDE
PotassiuM tartrate heMihydrate
BENZYL 2-CHLOROETHYL ETHER
2-Fluoro-3-pyridinecarboxaldehyde
2-Butoxyethyl acetate
(4-Fluorobenzyl)pyridin-3-ylmethylamine
7-Bromo-2,3-dioxo-1,4-dihydro-pyrido[2,3-b]pyrazine
3,3'-Dichlorobiphenyl 4,4'-Diisocyanate
Dichloro(di-?chloro)bis[1,3-bis(2,6-di-i-propylphenyl)imidazol-2-ylidene]dipalladium(II), 97%
2-Benzimidazolecarboxylic acid
Barium boron oxide
2,6-DIFLUORO-4-METHOXYBENZONITRILE
3-Chloro-4-fluorobenzaldehyde
Tributyl(1-ethoxyvinyl)tin
3,5-Diacetoxybenzoic Acid
2'-Chloro-4'-broMoacetophenone
TetraMethylsilane, 99%
Oleic Acid, NF
4-Nitrobenzoic Acid PotassiuM Salt
3-Phenylheptamethyltrisiloxane
4-chloro-N'-hydroxybenzenecarboximidamide
Bromomethylthiophene
[29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]iron
Tropic acid
4-Propoxycarbonylphenylboronic Acid
N-(3-BroMophenyl)-2-chlorobenzaMide, 97%
Bis(pentaMethylcyclopentadienyl)cobalt(III) hexafluorophosphate, 98%
2,3,6-TRIFLUOROPYRIDINE
N,N,N-TriMethyl-2-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)benzenaMiniuM iodide
Silver Mesylate
1-AMINOHEPTANE
4-IsopropylpyriMidine-5-boronic acid
2-Fluoro-6-methoxybenzaldehyde
1-(4-Iodophenyl)
[1,4-Bis(diphenylphosphino)butane]palladium(II) Dichloride
5-Ethoxy-2-mercaptobenzimidazole
ISOQUINOLINE-3-CARBOXYLIC ACID HYDRATE
Diammonium bis[carbonato-O]dihydroxyzirconate
3-Bromophenylacetic acid
3,4-Diaminotoluene
1,3-Dichloro-5-iodobenzene
Benzyl 4-Nitrophenyl Carbonate
4-(N-PropylaMinocarbonylMethyl)phenylboronic acid
Hydroquinone sulfonic acid, potassiuM salt, tech.
3-(4-Methoxyphenyl)-1H-pyrazole-4-carbaldehyde, 95%
L-Tyrosine Ethyl Ester
4'-Chloropivaloanilide
2-Cyclopropylethanol
Diphenan
4,6-DIBROMO ISATIN
2-N-Boc-amino-3-formylpyridine
1-(5-CHLORO-2-HYDROXY-4-METHYLPHENYL)-3-PHENYL-1,3-PROPANEDIONE
PotassiuM nitrate
METHYL BENZIMIDATE HYDROCHLORIDE
1,1,2,2,3,3-HEXAFLUOROPROPANE-1,3-DISULFONIMIDE POTASSIUM SALT
3-BroMo-4-Methylbenzoic acid
4-Keto-L-proline hydrobromide
2-Methylallylamine hydrochloride 98%
4-Bromo-4'-fluorobenzophenone
2-Bromopyridine N-oxide hydrochloride, 97%
5-(3-Bromophenyl)-1H-tetrazole
ETHYL 5-METHOXYINDOLE-2-CARBOXYLATE
3-Fluoro-2-nitrophenol
Phenyltrimethylammonium tribromide
1,2-Phenylenediacetic acid
2',3'-Dihydroxy-4'-Methoxyacetophenone Hydrate
(2-BroMophenethoxy)(t-butyl)diMethylsilane
Scopoletin, 95%
Isopropyl Alcohol, 99 Percent, USP, EP, BP, JP
LysozyMe Chloride froM Egg white
3-Acetamidobenzotrifluoride
4-Hydroxy-2,5-dimethyl-3(2H)-furanone
Methyl carbamate
Ethyl Methacrylate
Porcine Vascular Endothelial-Cadherin Complex,VE-cad ELISA Kit
POMC ELISA Kit
Human LIPT2 ELISA Kit
Human CR ELISA Kit
MBP ELISA Kit
Rat RGS4 (Regulator Of G Protein Signaling 4) ELISA Kit
ADP ELISA Kit
Human FGF9 ELISA Kit
Human Thymosin ELISA Kit
CP/CER ELISA Kit
Sabouraud’s Glucose Broth Medium
Charcoal Agar
Indole-5-Carboxylic Acid
FEMA 2200
5-AMinovaleric acid, 98%
2-Methyl-3-Buten-2-ol
3-Phenylheptamethyltrisiloxane
Piceatannol
3-Methoxypyridine
Dichloromethylphenylsilane
INDIUM FLUORIDE
4-Fluoropropiophenone
1,4-DibroMobenzene, 98%
3,4,5-Trichlorothiophene-2-carboxylic acid, 98+%
1-(3-Fluorobenzyl)hoMo, 95%
4-Phenylphenol
2,5-DIOXAHEXANEDIOIC ACID DIETHYL ESTER
3-ETHOXYBENZONITRILE
2-PHENOXYPROPANOL
2-NITROPHENYL ISOCYANATE
2,5-DIMETHOXY-3-NITROBENZOIC ACID
Pyrrolidone hydrotribromide, 97%
1,3-Dibromo-4-fluorobenzene
1-(3-Bromopropoxy)-2-fluorobenzene
Piperidine-2,4-dione