6T-CEM细胞株|人T淋巴白血病细胞系优惠力度大 货号:GD-C9719145
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-AMino-4-hydroxybenzenesulfonaMide, 95%
VincaMine
Solvent Red 43
N-(HydroxyMethyl)benzaMide
CALCIUM GLYCEROPHOSPHATE HYDRATE
4-(MaleiMido)benzophenone
4-BroMo-5-(3-chlorophenyl)pyrazole
2-Methoxybenzenesulfonyl chloride
n-Butylsulfonyl chloride
Cerium(III) sulfate octahydrate (99%-Ce) (REO)
N-Acetyl-o-phenetidine
3-Carboxyphenylboronic acid
2-BROMO-2'-HYDROXYACETOPHENONE
2-Iodobenzonitrile
3-Chlorobenzyl chloride
BroMoacetonitrile
2,5-DiMethoxybenzonitrile
SodiuM Vinylsulfonate (25% in Water, ca. 2.3Mol/L)
Tris(hydroxyMethyl)aMinoMethane hydrochloride, 99+%, extra pure
SiMvastatin, USP
5-Diazo-2,2-diMethyl-1,3-dioxane-4,6-dione
4-Hydroxybenzyl alcohol
acetylthiocholine chloride
O-phosphocolamine
4-Fluoro-2-nitrophenol
(-)-Corey lactone 4-phenylbenzoate alcohol
Janus Green B
Geraniol
6-Methylpyridine-2-carboxaldehyde, 98%
4-AMino-3,5-diMethyl-1,2,4-triazole, 98%
3-Carboxy-4-chlorobenzeneboronic acid, 98%
(S)-(+)-3-(Benzyloxycarbonyl)-5-oxo-4-oxazolidineacetic acid
2-DiMethylaMinoethyl chloride hydrochloride
PHENYL ISOTHIOCYANATE
2-Chloro-4-Methylbiphenyl-3-carboxylic acid
5-Carboxyphthalide
5-Nitroindazole
2,6-Dimethoxybenzonitrile 97%
2,4,6-Tris(pentadecafluoroheptyl)-1,3,5-triazine [for Mass spectrometry]
3-Chloro-4-methoxyacetophenone
4,4'-Dinitrobiphenyl
1-BROMO-2-NAPHTHALDEHYDE
N-Boc-L-alaninol
2-(DIFLUOROMETHOXY)BENZOIC ACID
Hexamethylphosphorous triamide
4-Chloro-4'-fluorobutyrophenone
Diisobutyl Ether
3-BroMo-5-(4-Methoxybenzyloxy)pyridine
Poly(caprolactone) diol, average M.N. 2000
Ferric AMMoniuM Sulfate, Dodecahydrate, Reagent, ACS
4-tert-Butoxybenzaldehyde
3-hydroxy-2,7-naphthalenedisulfonic acid, disodium salt
2-Amino-5-bromothiazole
Myricetin
tris(p-tert-butylphenyl) phosphate
2-Bromoacetophenone
t-Butyl 5-broMonicotinate
3-NitrophthaliMide
N-PhenylMaleiMide
Cyclobutane-1,1-dicarboxylic acid ethyl ester
4-Diazodiphenylamine Sulfate
Diphenylmercury, min. 96%
Tetrahydro-1,1,3-trioxo-2H-1,2-thiazine 97%
2-Chloro-N-methylacetamide
METHYL 3-AMINOBENZOATE
TRANS-2-METHYL-2-BUTENAL
2-Iodopropane
2-Morpholinobenzoic acid
1-AMinohydantoin Hydrochloride
Methyl (S)-(-)-2-Isocyanato-3-Methylbutyrate
2-BroMo-4-fluoro-1-(MorpholinoMethyl)benzene
2-Thien-2-ylbenzaldehyde, 97%
Sodium aluminum phosphate
1-AdaMantylthiourea
Gallic acid monohydrate
2-Bromo-1-(2,3-dihydro-1,4-benzodioxin-5-yl)-1-ethanone
6-methoxy-2,3-dihydro-4H-chromen-4-one
N-Methylstearylamine
SNAP-25 ELISA Kit
FOS ELISA Kit
Human APOC3 ELISA Kit
Human DEFβ114 ELISA Kit
Chicken CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit
Rat RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B) ELISA Kit
PPP ELISA Kit
Human monoamine oxidase,MAO ELISA Kit
ANGPTL4 ELISA Kit
TX-A2 ELISA Kit
Fluid Soybean-Casein Digest Broth
Medium 2(Penassay Base Agar)
Tin(IV) isopropoxide isopropanol adduct, 98% (Metals basis)
SodiuM fuMarate, 98%
5-Chloro-1-pentyne, 98%
2-Fluoro-5-Nitrophenylboronic Acid
4-Hydroxybenzyl alcohol
2,4-DICHLORO-5-AMINO-6-METHYLPYRIMIDINE
prolidase from lactococcus lactis
4-Aminotrifluorotoluene
3-(2-Chloro-6-fluorophenyl)-5-methylisoxazole-4-carboxylic acid
L-BETA,GAMMA-DIPALMITOYL-ALPHA-PHOSPHATIDIC ACID DISODIUM SALT
Triethyl Orthobutyrate
4-BroMo-3,5-difluoroanisole, 98%
2-(4-Pyridyl)-7-azabenziMidazole, 97%
Molecular sieves, 3 Å
Rhein
4-BroMo-3-Methylphenol
6-Fluoro-1-phenylbenzoiMidazole
exo-5-Norbornenecarboxylic acid
1,3-Bis(vinylsulfonyl)-2-propanol , 98%
N-(4-Nitrophenylsulfonyl)-L-phenylalanyl Chloride [Optical Resolving Reagent]
4-(4-Ethoxyphenoxycarbonyl)phenyl Ethyl Carbonate
1,1,2,2-Tetramethylcyclopropane
2,2,4-Trimethyl-1,3-dioxolane