ME-180细胞株|人子宫颈表皮癌细胞系优惠力度大 货号:GD-C9719101
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
TriethylaluMinuM, 25% w/w in hexane, 95%
SodiuM CyclaMate
N-Boc-D-Valino
METHYL 4-IODOSALICYLATE
Ethyl 6-hydroxyhexanoate
1-BroMo-4-fluoro-2,5-diMethylbenzene
8-HEXADECYNE
(+/-)-Camphene (contains ca. 20% Tricyclene)
1,2,5-Oxadiazole-3-carboxylic acid 97%
Ammonium molybdate tetrahydrate (99.98%-Mo)
9,9-Bis[4-(2-hydroxyethoxy)phenyl]fluorene
1-Benzyl-1H-pyrazole-4-boronic acid pinacol ester
DIETHYL 2,2-DIETHOXYETHYLPHOSPHONATE
1-PYRENEMETHANOL
2-Chloro-4-hydroxybenzoic acid hydrate
4-(ChloroMethyl)-2,5-diMethyl-1,3-oxazole
1,6-DibroMododecafluorohexane
Methyl 6-aMino-3,5-dibroMopicolinate
Tri-n-propylaluMiniuM, 0.7M solution in heptane, AcroSeal
Resorcinol, Reagent
2-Chloro-4,5-difluorobenzoic Acid
2,2-diethoxyethanol
(Carbethoxyethylidene)triphenylphosphorane
2,4-diamino-6-hydroxymethylpteridine
4-fluoroindole
(R)-(-)-Methyl glycidyl ether
4-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE
2,3-DibroMo-5-Nitro Pyridine
(1R,2R)-N,N-Bis[2-(di-p-tolylphosphino)benzyl]cyclohexane-1,2-diaMine, 97%
2'-Hydroxy-4',5'-diMethylacetophenone, 98%
2-BroMobenzenesulfonaMide, 99%
PotassiuM heptafluorotantalate(V)
N-(3-HYDROXYPROPYL)PHTHALIMIDE
PICOLINIC ACID HYDROCHLORIDE
CHLOROACETYL-L-TYROSINE
1,2,6-Hexanetriol
3-PHENOXYTHIOPHENE
3,4'-Dihexyl-2,2'-bithiophene
4-tert-Butyl-3-methoxy-2,6-dinitrotoluene
1-[2-(1H-1,2,4-triazol-1-yl)phenyl]methanamine(SALTDATA: HCl)
1,2-Nonanediol
(+)-Abscisic acid
RHODAMINE 110
Dimethyl phthalate
Methyl (R)-(-)-3-hydroxybutyrate
2-(4-Chlorophenyl)-4-Methyl-1,3-thiazole-5-carbonyl chloride
2,2'-Dihydroxydiphenyl Ether
5-BroMo-3-nitropicolinonitrile
SalicylaMide, 99%
L-Arginyl-L-aspartic Acid
Myristic Acid
3-Amino-5-methoxybenzotrifluoride
(R)-2-methylsuccinic acid
Triethylsilanol
2,4-Dinitroaniline
TRIPHENYLPHOSPHINE DIBROMIDE
Metanilic acid
DiaMMoniuM Dithiodiglycolate (45% in Water)
4-CHLORO-2,5-DIFLUOROBENZOIC ACID
Erbium chloride hydrate, (trace metal basis), 99.997%
Dimethyldifluorosilane, tech
7-Bromo-2,3,4,5-tetrahydro-1H-benzo[e][1,4]-diazepine-2-carboxylic acid methyl ester
N-(N-Trifluoroacetyl-L-cysteinyl)glycine methyl ester
4-Ethoxycarbonylphenylboronic acid
BUTYL ANTHRANILATE
5-Chloroindole-3-carboxylic acid
Tetraoctylammonium bromide
3,5-DibroMopyridine
1-AcetaMido-4-hydroxyanthraquinone
3-BroMo-4-ethoxypyridine
3-Methyl-1-butanol, 99%, for biocheMistry, AcroSeal
trans-N,N'-DiMethyl-1,2-cyclohexanediaMine
FMOC-TIC-OH
2,2,4,4-TetraMethylpentane
Methyl enanthate
N-benzoyl-(2R,3S)-3-phenylisoserine
3-Chloro-5-methoxyphenol
N-(3-chlorobenzyl)-N-methylamine
VD R ELISA Kit
Mouse α1-microglobulin,α1-MG ELISA Kit
PS2 ELISA Kit
Human nonsmall cell lung cancer/Lung tumor Antigen,LTA ELISA Kit
Chicken infectious coryza,ic ELISA Kit
Rat THP (Tamm–Horsfall Glycoprotein) ELISA Kit
C3 ELISA Kit
PIP ELISA Kit
Human Vascuolar cell adhesion molecule 1,VCAM-1 ELISA Kit
KDR ELISA Kit
N6 Medium
Glfu Anaerobic Medium Semisolid without Dextrose
LanthanuM(III) isopropoxide, La 40%
Glycine methyl ester hydrochloride
5-Chloro-2-Methoxynicotinic Acid
2-Fluorobenzyl alcohol, 98%
2,2-diethoxyethanol
Reactive Yellow 18
Dimethyl silicone oil 201
8-fluoroquinoline
TRANS-5-DECENE
3-Carboxy-5-nitrophenylboronic acid
NiobiuM Oxide
(+)-Dibenzoyl-D-tartaric acid, anhydrous, 99%
5-AMino-4-(4-fluorophenyl)-1H-pyrazole, 97%
Methidathion
L-Leucinol
Methyl (S)-(-)-2-Isocyanato-4-(Methylthio)butyrate , 98%
3-Methyl-2-pyridone
4-Fluoro-2-Methylthiobenzothiazole
2,4-DINITROBENZOIC ACID
3-Carene, stabilized, 90%
BQ-788.Sodium Salt
4-Isopropoxyaniline
8-Benzyloxy-5-(2-bromoacetyl)-2-hydroxyquinoline