MDA-PCA-2B细胞株|人前列腺癌细胞系 货号:GD-C9318285
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞,在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-(2,4-DiMethylphenylcarbaMoyl)benzeneboronic acid, 98%
Triethyl orthoforMate
1,2,4,5-Benzenetetracarboxylic anhydride
Methyl 4-vinylbenzoate
4-BROMO-3,5-DIHYDROXYBENZAMIDE
4-(ChloroMethyl)benzoyl chloride
4-(TrifluoroMethylthio)phenyl triflate
Neohesperidose ,98%
Decyl isothiocyanate
Potassium (3,4-dichlorophenyl)trifluoroborate 96%
(R)-(+)-1,1,2-Triphenyl-1,2-ethanediol
Apraclonidine
2-Chloro-4,5-difluorobenzonitrile
2,6-Diaminotoluene-4-sulfonic acid
4-Isopropylbenzeneboronic acid
Rosin
Dibutyl N,N-DiethylcarbaMoylMethylphosphonate [for Extraction of Lanthanides and Actinides]
5-BroMo-2-(MethoxycarbonylaMino)pyridine
Poly(ethylene glycol), average M.W. 400
Ferric Sulfate, Technical
1H-Cyclopenta[l]phenanthrene
naphthol as-D
1-Methyl-5-nitroimidazole
2-Methoxybenzoic acid
tris(2,3-dichloropropyl) phosphate
Cyclopentanemethanol
2-Allylcyclohexanone
4-AMino-4-Chlorodiphenyl Ether
2-(Boc-aMino)benzeneboronic acid pinacol ester, 97%
Methyl-p-benzoquinone, 98%
Siduron
Dithiopyr
Methyl 2-Methyl-3-nitrobenzoate
Zinc peroxide
N-BENZYL-N-BUTYLAMINE
1H-1,2,4-Triazole-1-CarboxaMidine
5-Hydroxyisophthalic Acid
4-[4-(Hydroxymethyl)pyridin-2-yl]morpholine 97%
5-Morpholin-4-ylmethyl-furan-2-carboxylic acid
fmoc-d-gln-opfp
2,3,5-Tribromothiophene
NITRILOTRIACETIC ACID DISODIUM SALT
3,4,5-Trifluorobenzaldehyde
1,2,3,4-Tetrahydroquinoline;Py-tetrahydroquinoline
p-Anisidine
4-(DiMethylaMino)phenylboronic acid
5-Norbornene-2,2-diMethanol
Z-Lys(tfa)-oh
(2S)-4-(1,3-Dioxoisoindolin-2-yl)-2-hydroxybutanoic acid, 96%
Sodium bromide
2-(EthylaMino)pyridine
2-Hydroxy-5-nitro-3-picoline
4-(trifluoromethoxy)phenylacetonitrile
(n-Propyl)triphenylphosphonium
(S)-3-hydroxy-gamma-butyrolactone
4-Benzoylbiphenyl
FMoc-N-Me-Ile-OH
2-(Methoxycarbonyl)nicotinic acid
ChloroMethyl chlorosulfate
Perfluoroundecanoic acid
1,3-Phenyldiboronic acid, bis(pinacol) ester, 97%
Methyl Nonadecanoate
(S)-4-(2,3-Epoxypropoxy)-9H-carbazole
5-Cyanophthalide
2'-FLUORO-3'-(TRIFLUOROMETHYL)ACETOPHENONE
Methyl methoxyacetate
;
3-BroMo-2-Methylbenzoic Acid
(+)-Abscisic acid
HexyldiMethyloctylaMMoniuM BroMide
Boc-N-Me-Thr-OH
2,1,3-Benzoxadiazole, 97%
3,5-Di-tert-butyl-4-hydroxybenzoic acid
trans,trans-2,4-Decadienal
benzbromarone
Ethyl 2-amino-2-methylpropanoate hydrochloride
alaninamide
3-cyanobenzoyl chloride
HIS ELISA Kit
ADMA ELISA Kit
Human CTSV ELISA Kit
Human BMP-15/GDF-9B ELISA Kit
6KPGF1a ELISA Kit
IL-2 ELISA Kit
ES-Ab ELISA Kit
Human GAL8 (Galectin 8) ELISA Kit
Human COX ELISA Kit
Sil-6R/cd126 ELISA Kit
Bromcresol Purple Dextrose Broth
H Medium
PERILLIC ACID
Flavin adenine dinucleotide disodiuM salt hydrate, 94% (dry wt.), water <10%
5-BroMo-2-chloro-3-nitropyridine, 98%
2-Hydroxy-Cyclopentanecarboxylic Acid Methyl Ester
naphthol as-D
o-Methoxycinnamic acid
1-azabicyclo[2.2.2]oct-3-ylmethanol
Isobutyric acid
2,5-DIMETHYLBENZYL ALCOHOL
Miconazole nitrate
4-Hydroxyquinazoline
2-BroMo-6-iodo-4-(trifluoroMethyl)anisole, 97%
1-Tetradecylphosphonic acid, 98%
trans-2,4-DifluorocinnaMic acid
Tetrahydro-2,2-diMethyl-4H-pyran-4-one
4'-Hydroxy-3'-Methylacetophenone
4-Iodo-3-nitrobenzoic acid
N-AcetylneuraMinic acid
3-Borono-N-(2,3-diMethylphenyl)benzaMide
h-his(trt)-ome.hcl
Ethyldimethylsilane
1,4,8,11-Tetraazacyclotetradecane-N,N',N'',N'''-tetraacetic acid, tetraethyl ester, min. 98%
Boc-N-Me-Phe.DCHA