RBL-1细胞株:大鼠嗜碱性粒细胞系无污染 货号:GD-C8819164
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞,全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-tert-Butylphenol, 99%
4-BroMoMethylphenylboronic acid pinacol ester
Methyl dodecylbenzenesulfonate
4-Fluoro-3-(trifluoroMethyl)benzaMide, 97%
(R)-DTBM-SEGPHOS
POTASSIUM CHLORIDE RUTHENATE
BENZO[B]THIOPHENE-2-CARBOXALDEHYDE
Acetovanillone ,99%
2,2,2-Trifluoroethyl methacrylate
Tin(II) fluoride 99%
1,2-Naphthoquinone-2-diazido-5-sulfonic Acid Sodium Salt [Research for Photosensitive Material]
3-Piperazinyl-1,2-benzisothiazole hydrochloride
1-(2-DIISOPROPYLAMINOETHYL)
3-Isoquinolinecarbonitrile
tert-Butyl 4-[2-(aMinoMethyl)phenyl]-1-carboxylate
HeMin
3,5-DiMethyl-1-adaMantanol
Valsartan
TryptaMine hydrochloride, 98%
SodiuM Borate, Decahydrate, Reagent, ACS
Bis(NHS)PEG5
Ethyl glycolate
Ethyl 4-bromocrotonate
3-ACETYLPYRROLE
5-fluoro-2-nitrobenzaldehyde
2,4-DIAMINO-6-MERCAPTOPYRIMIDINE
BIS(DICYCLOHEXYLPHOSPHINO)METHANE
4-AMinobenzoic Acid
Mercury(II) sulfide, black
2-Fluoropyridine-3-boronic acid pinacol ester, 95%
5-Chloro-1H-pyrrolo[2,3-b]pyridine
PCB No 15
4-(DiMethylaMino)phenylboronic acid, HCl
PHENACYL 4-BROMOCROTONATE
2-BroMophenol
Tetrachlorosilane
4-(1,3-Thiazol-2-yl)benzaldehyde, 95%
3-Chlorothiophene-2-carboxylic acid
Hyaluronic Acid from Cockscomb
5-Bromo-2-hydroxypyrimidine
5-Methylthiazole
3,5-Dichlorobenzoic acid
L-Ornithine 2-oxoglutarate
3,3'-DIMETHOXYBENZIDINE
3-Hydroxy-1-adaMantanecarboxylic acid
N,N-DiisopropylethylaMine trihydrofluoride
Hinokitiol
1-Benzylpiperidine-2-carboxylic acid
3,5-DiMethylphenylMagnesiuM broMide, 0.5M solution in THF, AcroSeal
4-Chloro-2-nitrobenzaldehyde
SodiuM 1-Pentanesulfonate
1-Methyl-3-phenyl-2-thiourea
P-iodo-dl-phenylalanine
4-Chloro-2-picoline
Cyclobutanone
TETRA-N-DODECYLAMMONIUM IODIDE
5-(Di-tert-butylphosphino)-1-(naphthalen-1-yl)-1H-pyrazole
BENZENESULFONIC ACID 2-CHLOROETHYL ESTER
CalciuM bis(2-hydroxy-4-(Methylthio)butyrate)
Cyanopyrazine
8-Hydroxy-1,4-dithiaspiro[4.5]decane
(R)-(+)-2,2',6,6'-Tetramethoxy-4,4'-bis(diphenylphosphino)-3,3'-bipyridine, min. 95% CTH-(R)-P-Phos
4-[4-(Oxiran-2-ylmethoxy)phenyl]-1,2,3-thiadiazole, 95%+
BARIUM 2-CYANOETHYLPHOSPHATE
N-Fmoc-D-1,2,3,4-Tetrahydroisoquinoline-3-carboxylic acid
2-(Chloromethyl)benzonitrile
Methoxyacetone
Methyl 2-chloro-4,4-diMethyl-3-oxopentanoate
1-Benzyl D-Aspartate
3-(CyclopropylMethyl)thiophenylboronic acid
cis-DichlorodiaMineplatinuM(II), 99.99%, (trace Metal basis)
2-BroMothiophene-3-carbonitrile, 97%
1-Octadecyne
1,2,3,4-Tetrahydroisoquinoline
acetaldehyde 2,4-dinitrophenylhydrazone
4-(Aminomethyl)-3-pyridinamine hydrochloride (1:1)
Ethyl oxazole-5-carboxylate
4-Fluoro-2,3-Dimethylphenol
FTH ELISA Kit
TLR-5 ELISA Kit
Human APOB ELISA Kit
AhR ELISA Kit
IL5 ELISA Kit
Rat α1-Acid glycoprotein,α1-AGP ELISA Kit
IFN-γ ELISA Kit
MCP-1 ELISA Kit
VWF ELISA Kit
PA-lgM ELISA Kit
Tryptic Soy Agar
Mycoplasma Broth Base(Frey)
GalliuM(III) isopropoxide, Mixture of oligoMers, 99%
SodiuM hyodeoxycholate, 98%
5-BENZYLOXYINDOLE-3-ACETIC ACID
2-FLUORO-5-HYDROXYPYRIDINE
Ethyl glycolate
solanesol
3,4-Dihydroxypyridine
2-Fluoro-5-nitrotoluene
Quipazine
N-4-Boc-2-acetic Acid Methyl Ester
4-Methyl-2-pentanone, 99%
(R)-3-(Boc-aMino)-4-(4-iodophenyl)butyric acid, 95%
3-Methylcyclopentane-1,2-dione, 98+%
IsopropylaMine
8-Methylquinolin-4(1H)-one
TRIPHENYLBISMUTH CARBONATE
2,4-DIAMINO-6-HYDROXY-5-NITROSOPYRIMIDINE
2-METHYLACETANILIDE
DILITHIUM PHTHALOCYANINE
2-(4-Chlorophenoxy)nicotinamide
3,5-Dimethylpyrazole-1-methanol ,98%
Disodium 4-Formylbenzene-1,3-disulfonate
3-Methyl-2-phenylpyridine