NUGC-2细胞株：人低分化胃癌腺癌细胞系培养条件 货号：GD-C8620029
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-BroMo-N-cyclohexylbenzenesulfonaMide, 97%
2,3-DibroMo-5-Methylpyridine
TetraphenylMethane
2-(Phenylsulfonyl)-1,3-cycloheptadiene
5-BroMo-2-(Methylthio)benzothiazole
N-Benzyl-N-MethylethanolaMine
4-ChloroMethylphenylboronic acid
2-Amino-5-diethylaminopentane
4-Methylamino-1,1-dioxo-tetrahydro-thiophen-3-ol
2-Cyclohexylcyclohexanol
L-Valylglycine
Cholesteryl palmitate
Etiocholanone
2-(DICYANOMETHYLENE)INDAN-1,3-DIONE
ANHYDROTETRACYCLINE HYDROCHLORIDE
4-(Tetrahydropyran-4-yloxy)benzoic acid
8-DiMethylaMino-1-octanol
3-Chloro-6-(4-iodo-1H-pyrazol-1-yl)pyridazine
TetraMethylthiuraM disulfide, 97%
Nonidet(R) P40 Solution
6'-(DiethylaMino)-1',3'-diMethylfluoran
trizinc dicitrate
L-Tyrosinol
3-Methyl-1,2-cyclopentanedione
pheniramine maleate
2,6-DIMETHYLBENZONITRILE
2-Bromochlorobenzene
3-(4-BroMobenzoyl)Propionic Acid
4-tert-Butylphenoxyacetic acid, 98%
2-Hydroxy-4-Methylquinoline, 98%
Ethyl fluoroacetate
8-Fluoro-4-hydroxy-2-(trifluoroMethyl)quinoline
POTASSIUM PENTACHLORONITROSYLRUTHENATE(II)
SILVER ARSENATE
2-Chloro-6-fluoro-3-Methoxyphenol
O-PHOSPHO-L-TYROSINE
n-Butyl acetate, AcroSeal?, Extra Dry, 99+%
DL-Phenylsuccinic acid, 98+%
Biphenyl-2,2'-dicarboxaldehyde
(1S)-(-)-beta-Pinene, 98%
SILICON TETRABROMIDE
2,6-Difluorophenylboronic acid
5-BROMO-INDOLE-3-BUTYRIC ACID
ThioacetaMide;EthanethioaMide
4-Chloro-1-Methyl-1H-pyrazolo[3,4-d]pyriMidine
2-Naphthalenethiol
3'-Sialyllactose SodiuM Salt
1-DiMethylaMino-2-propyne, 97%
1-[4-(TrifluoroMethyl)pyriMidin-2-yl]-1,4-diazepane, 95%
Menadione
5-BroMo-2-fluorobenzaldehyde
palatinitol
Glycerides, mixed decanoyl and octanoyl
thieno[3,2-b]thiophene-2-carbaldehyde
hexafluorophosphoric acid
Isobutyl iodide
6-AMino-1,3-diMethyl-1,2,3,4-tetrahydropyriMidine-2,4-dione
Flopropione
1-Boc-5-Methoxyindole-3-boronic acid, pinacol ester
Adapalene
2,4,5-Tribromoimidazole, 97%
1-Phenoxy-2-propanol
2,4-Dichloro-3,5-dinitrobenzotrifluoride
Ibudilast
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt
Heptyl chloroformate
8-Hydroxyquinoline sulfate;8-Quinophenol sulfate;Oxine sulfate;Quinosol;Chinosol
2-(Tetrahydro-2-furanMethoxy)-1-propanaMine
1,2-Propyleneglycol diacetate
Fast Red ITR Base
4-Chloro-1h-pyrrole-2-carboxylic acid
2-BroMo-3-pyridinecarboxaldehyde, 96%
PROWL
Octadecyl Thioglycolate
6-Trifluoromethyloxindole
Hexaaminecobalt(III) chloride
1,1-dichlorosilacyclohexane
p-iodoanisole
CDH5 ELISA Kit
IKK ELISA Kit
Human FADS3 ELISA Kit
Human CAPN6 ELISA Kit
AREG ELISA Kit
Rat RGS4 (Regulator Of G Protein Signaling 4) ELISA Kit
Monkey ADP/Acrp30 (Adiponectin) ELISA Kit
Human FGFBP1 (Fibroblast Growth Factor Binding Protein 1) ELISA Kit
Human Thymosin ELISA Kit
HA ELISA Kit
1% Tween80-Corn Meat Agar Medium Base
BAT Agar
Indole-5-carboxylic acid
Ethyl Heptanoate
5-AMINO-1H-TETRAZOLE
2-Methyl-3-butyn-2-ol, 98%
trizinc dicitrate
Trimethylsilylmethanesulfonate
trimesitylborane
Gallic acid
Tetrabutylammonium iodide
5-Aminoindole
7-BroMoindole
D-Arginine, 98%
4-Hydroxy-3-oxo-2,5-diphenyl-2,3-dihydrothiophene 1,1-dioxide, 98%
Boc-D-Pro-OH
Ranatensin
2-Phenylphenol
1,2,3,4-TETRAHYDRO-1-ACETYLQUINOLINE
TributylaMine
1-Trityl-1H-iMidazole-4-Methanol
2-Methyl-2-nitro-1,3-propanediol
3-(Hydrazinecarbonyl)benzeneboronic acid 97%
2-pyrrolidin-2-ylpyridine(SALTDATA: 2HCl)
1-(1-phenylethyl)-1H-pyrazol-5-amine(SALTDATA: FREE)
