AML-193细胞株：人急性单核细胞系培养条件 货号：GD-C8619096
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-Benzyloxy-3,5-diMethylbenzoic acid, 98+%
ADA sodiuM salt
3,4-Dichloroaniline-6-sulfonic acid
2-Ethoxypyridine-3-boronic acid pinacol ester
TRIMETHYLACETIC ANHYDRIDE
Dibenzosuberone
Stevioside
4-Phenylthiosemicarbazide
1,5-Dihydroxy-4,8-dinitroanthraquinone
Chlorocarbonylbis(triphenylphosphine)iridium(I), 99% VASKA'S COMPLEX
2,4-Bis(tert-butyl)-6-bromophenol
tert-Butoxycarbonyl-L-valine N-hydroxysuccinimide ester
4-BROMO-3,5-DIMETHOXYBENZOIC ACID
17-Hydroxy-7-alpha-mercapto-3-oxo-17-alpha-pregn-4-ene-21-carboxylic acid-gamma-lactone-7-acetate
Methyl 4-pyrrolidin-1-ylbenzoate
Thioglycolic acid
4,10-Dioxatricyclo[5.2.1.0(2,6)]decan-8-en-3-one
N-(3-Methoxypropyl) 3-broMo-5-trifluoroMethylbenzenesulfonaMide
Tri-n-octylaluMiniuM, 0.3M solution in heptane, AcroSeal
Quinidine Sulfate, Dihydrate, USP
5-Acetyl-1,3-diMethylbarbituric Acid
3-nitrophenylacetonitrile
(S)-(-)-3-Cyclohexenecarboxylicacid
L-Homophenylalanine
2,6-Difluorobenzoic acid
METHYL 2,5-DICHLOROBENZOATE
Hexaphenylcyclotrisiloxane
4-Iodobiphenyl
2-Benzoylthiophene, 98%
5-Fluoro-2-hydroxy-N,N-diMethylbenzylaMine, 99%
TriMethylphenylaMMoniuM broMide
Linalyl Acetate
LithiuM tantaluM isopropoxide, 99.9% (Metals basis)
PipecolyldithiocarbaMic Acid PipecoliniuM Salt , 98%
2',3',5'-Triacetylguanosine
3-AMino-5-fluoropyridine
4-(N-Acetylsulphamoyl)benzeneboronic acid 97%
2,4-Dimethyl-3-pentanone
Palmitoleic acid, 99%
2-(2-nitrophenoxy)propanoic acid(SALTDATA: FREE)
2,4-Dimethoxy-1-nitrobenzene
2-ACETOXYACETOPHENONE
THIANAPHTHENE-2-CARBOXAMIDE
4-Fluoro-3-nitrobenzotrifluoride
2,4-Dichlorobenzyl chloride
4-Chlorobenzyl broMide
1-(3-Hydroxypropyl)-2-pyrrolidone
4,5-Difluoro-2-nitrotoluene
2,6-DifluorobenzylaMine, 97%
Isopropyl palmitate
4-(2-AMinoethyl)benzenesulfonyl Fluoride Hydrochloride [for BiocheMical Research]
2-(Chloromethyl)oxolane
cis-Dichlorodiamineplatinum(II)
1,3-Dioxane, 2,2-dimethyl-5-nitro-
dihydrocoumarin
1,4-Dicyanobutane
PR(TMHD)3
3-(ChloroMethyl)pentane
4-BroMo-2-fluorotoluene
(3R)-(+)-3-(ETHYLAMINO)PYRROLIDINE
7-Acetoxy-4-methylcoumarin
1H,1H,2H-Perfluorocyclopentane
Ethyl Phenyl Sulfide
5-[4-(tert-Butyl)phenyl]-1H-1,2,4-triazole-3-thiol
ISOCYANOBENZENE
2-FLUORO-DL-PHENYLALANINE
FMOC-SUBEROL
4-Hydroxy-2-(trifluoroMethyl)pyriMidine
18-Crown 6-Ether
4,6-DiMethyl-2-(Methylsulfonyl)pyriMidine
Voriconazole
TryptaMine, 98%
SodiuM Borohydride, Crystalline Powder, Reagent, ACS
5-(4-Chlorobutyl)hydantoin
Ethyl 2-mercaptoacetate
2,2-dimethyl-propanedioic acid dimethyl ester
2-acetylpyrrole
4-Fluoro-2-methoxybenzoic acid
CDX2 ELISA Kit
α2-MG ELISA Kit
Human EBF1 ELISA Kit
MCT ELISA Kit
LH ELISA Kit
TIEG1 ELISA Kit
Chicken Complement 3 split product,C3SP ELISA Kit
PRL ELISA Kit
Human Vascuoar endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA Kit
ATA ELISA Kit
Murashige & Skoog Micronutrients
Anaerobic Broth Base
NEODYMIUM(III) ISOPROPOXIDE
Glycine tert butyl ester hydrochloride
5'-Chloro-2'-hydroxy-3'-nitroacetophenone, 99%
2-FLUORO-DL-PHENYLALANINE
3-nitrophenylacetonitrile
Reactive Orange 20
Amylose
2-Fluorobenzonitrile
(4-Pyridylthio)acetic acid
4-Chlorobenzophenone
1,2-Propyleneglycol Diacetate
7-BroMo-1-chloroisoquinoline, 97%
(R)-(-)-3,3-DiMethyl-2-butylaMine, ChiPros, 99+%, ee 99+%
ErbiuM oxide
Hydroiodic acid
(S)-(+)-3-METHYL-1-PENTANOL
ALPHA-METHYL-GAMMA-BUTYROLACTONE
Graphite
2-AMino-4-ethoxycarbonylphenylboronic acid, HCl
L-Prolinol ,98%
7-Methoxy-3-phenylsulfonyl-1(3H)-isobenzofuranone
Chlorodifluoroacetyl chloride
Direct Yellow 44
