hFOB 1.19细胞株:人SV40转染成骨细胞系培养条件 货号:GD-C8618616
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项:1)如果细胞为贴壁细胞,而收到时呈悬浮或者部分悬浮的状态,请将悬浮的细胞即时离心,加15%血清的完全培养基到新的培养皿/瓶继续培养3天;同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡,请及时联系实验室技术人员会跟进解决;2)贴壁细胞生长缓慢;适当提高血清浓度(不能超过20%),或可根据该细胞生长密度,考虑胰酶消化后,转移到新的培养瓶继续培养;3)生长不均:贴壁细胞若出现分布不均,成岛状生长,可将细胞进行消化,重悬打散细胞,加入新鲜培养基进行培养。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-BroMo-5-fluorobenzaMide, 96%
CalciuM lignosulfonate
1,3-BUTADIENE DIEPOXIDE
4-(4-Methoxyphenoxy)piperidine, HCl
6-BROMO-O-CRESOL
SUCCINALDEHYDE SODIUM BISULFITE
Cyclohexanecarboxaldehyde
Ethyl isoquinoline-3-carboxylate, 97%
Ethyl 2-(4'-hydroxyphenyl)-1,3-oxazole-4-carboxylate
1-Bromo-4-fluoro-2-(methoxymethyl) benzene
cis-3-Hexenyl cis-3-Hexenoate
(2-CHLORO-5-TRIFLUOROMETHYL)PHENYLTHIOUREA
5-AMINOINDAZOLE
MANGANESE(II) ACETATE
m-Anisidine
4-Chloro-1-Methyl-1H-pyrazole-3-carbaldehyde
Benzyl DiethyldithiocarbaMate
4-(4-BroMophenyl)-2H-pyrazol-3-aMine
SodiuM hydroxide, extra pure, 50 wt% solution in water
L-Prolyl-L-valine
3-(4-BroMobenzoyl)propionic Acid
fenfluramine hydrochloride
4-Methyl Phenyl Thioacetic
2-Pyrrolidinone
4-Methoxyphenylacetic acid
1,3-DICHLORO-1,1,3,3-TETRAISOPROPYLDISILOXANE
Methyl 4-trifluoromethylbenzoate
2-Phenyl-2-Propanol
2-Chloro-4-(trifluoroMethyl)benzonitrile, 98%
2,4,6-Triiodophenol, 98%
Cordycepin
1,3-DiisopropyliMidazoliuM chloride
1-Methyl-1H-pyrazole-4-carboxylic acid
TetraMethylene sulfoxide
Dichlorprop
4-(2-Nitrophenoxy)phenylboronic acid
5-Fluoro-2-(1H-imidazol-1-yl)aniline, 97%
1-Naphthalenemethanol, 98%
4-Iodophenylacetic acid
Ethyl 2-Hydroxybutyrate
TRIMETHYLGERMANIUM CHLORIDE
Fosinopril sodium
Ethyl levulinate
Turpentine oil;Oil of turpentine
DichlorMid
2-Naphthol
Theophyllol
1-Iodohexane, 98+%, stabilized
2-Fluoro-5-iodopyridine, 97%
4-Nitrophenyl-beta-D-galactopyranoside
Food Red No. 102
Fatty acids, cottonseed-oil
4-Aminomethylphenylboronic acid hydrochloride
5-chlorothiazolo[5,4-b]pyridin-2-amine
tartaric anhydride dibenzoate
4,4'-Dichlorobenzophenone
3-BroMo-1-Methyl-5-(trifluoroMethyl)pyridin-2(1H)-one
4-BENZOYLOXY-TEMPO
Anthrone
1-(4-Fluorophenyl)cyclopentanecarboxylic acid, 98%
2,4,6-trichlorobenzothiazole
3-bromo-2-quinolinol(SALTDATA: FREE)
Diisobutyl Fumarate
VANADYL SULFATE HYDRATE
Butyl isovalerate
3-BROMO-2-BUTANONE
Cefoperazone Dihydrate
SodiuM isoascorbate Monohydrate
(S)-3-AMino-1-carbobenzoxypyrrolidine Hydrochloride
4-AMinophenylboronic acid
4-DiMethylaMinopyridine, 99%
3-(4-Chlorobenzoyl)propionic acid, 99%
D-Pantethine
2-(2-Fluorophenyl)ethanol
Guanidine propionate
(2-BOC-aminophenyl)boronic acid
3-Methyl-1,3,4,5-tetrahydrobenzo[d]azepin-2-one
N'-hydroxythiophene-2-carboximidamide
ACh ELISA Kit
IL5Rα ELISA Kit
Human recombination activating gene 1,RAG-1 ELISA Kit
Human LIPC ELISA Kit
LH ELISA Kit
Rat BAK1 (BCL2 Antagonist/Killer 1) ELISA Kit
Histon-H2b ELISA Kit
Human Complement 3,C3 ELISA Kit
AFP-L2 ELISA Kit
IP-2 ELISA Kit
de Man, Rogosa an Medium(Modified MRS Medium Base)
Violet Red Bile Agar
MucobroMic Acid
Erythromycin 2'-propionate dodecyl sulfate
5-Methylthiophene-2-boronic acid, 98%
2-Methoxy-3-nitropyridine-5-boronic acid pinacol ester, 96%
fenfluramine hydrochloride
N-methyl-N-(trimethylsilyl)acetamide
Supracet brilliant red BD
n-Butylamine
1H,1H,2H,2H-Heptadecafluorodecyl acrylate
Spiramycin
Benzenesulfinic Acid SodiuM Salt Dihydrate
Gold(III) oxide, PreMion|r, 99.99% (Metals basis), Au 88.6% Min
1-(4-Morpholinyl)cyclohexanecarbonitrile, 96%
SulfacetaMide
4'-BroMo-2-Chloroacetophenone
Boc-D-Pen(pMeOBzl)-OH
ZirconiuM carbide
3-AMino-3-(p-tolyl)propionic Acid , 98%
4-BroMo-4'-iodobiphenyl
3,5-Dimethoxyaniline ,98%
Tetrabromohydroquinone
2'-Hydroxy-4'-methoxypropiophenone
(R)-Prolinamide