PT67细胞株:小鼠逆转录病毒包装细胞系培养条件 货号:GD-C8618424
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项:1)如果细胞为贴壁细胞,而收到时呈悬浮或者部分悬浮的状态,请将悬浮的细胞即时离心,加15%血清的完全培养基到新的培养皿/瓶继续培养3天;同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡,请及时联系实验室技术人员会跟进解决;2)贴壁细胞生长缓慢;适当提高血清浓度(不能超过20%),或可根据该细胞生长密度,考虑胰酶消化后,转移到新的培养瓶继续培养;3)生长不均:贴壁细胞若出现分布不均,成岛状生长,可将细胞进行消化,重悬打散细胞,加入新鲜培养基进行培养。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3,3'-DiaMinobenzidine, 99%
Sudan Black B
1,1,3-TRIMETHYLCYCLOHEXANE
4-BROMOBENZYL MERCAPTAN
1-(2-BROMOPHENYL)-2-THIOUREA
SodiuM taurocholate
(S)-(?)-1-(1-Naphthyl)ethanol
2-[2-(2-Chloroethoxy)ethyl]-1H-isoindole-1,3(2H)-dione, 97%
5-Acetamido-2-bromopyridine 95%
n-Hexylsilane
3-Biphenyl-3',5'-difluoro-carboxylic acid
(S)-1-(3-METHOXYPHENYL)ETHYLAMINE
MERCURIC OXIDE, RED
Hexafluorozirconic acid
4,4'-Dimethyltriphenylamine
AMMoniuM tetrathioMolybdate
3',5'-Diacetoxyacetophenone
5-broMo-3-Methylpyridine-2-carbonitrile
Pulegone, 92%, pract.
HydroxocobalaMin
Docosane
imidazo[1,2-a]pyrazine
2-indanone oxime
2,3,4-trimethylpentane
4-Chloro-2-nitroaniline
4,6-Dihydroxy-2-methylpyrimidine
4-(2-Hydroxyethyl)Piperidine
2,4-DiMethylphenol, 98%
(S)-(+)-Phenyl-1,2-ethanediol, 97%
4-Chloro-N-isobutylbenzaMide, 97%
FMoc-Orn(Boc)-OH
4-(N-t-ButylaMino)-3-nitrophenylboronic acid, pinacol ester
5-Isopropylbarbituric acid, 98%
TriMethyl cis,cis-1,3,5-cyclohexanetricarboxylate
Tetranitroblue tetrazoliuM chloride
RhodiuM carbonyl chloride
(S)-3-(1-Aminoethyl)anisole
Glycyl-DL-valine
1-methyl-4-phenyl-1H-pyrazol-5-amine(SALTDATA: FREE)
Ruthenium(III) iodide, anhydrous, 98+%
o-Anisic acid
1,2-Dodecanediol
3,5-DIFLUOROBENZOPHENONE
Diethylaminosulfur trifluoride
Benzaldehyde
1-Benzyl-5-phenylbarbituric Acid
3-CyanoMethoxyphenylboronic acid, pinacol ester
Chloroxazone, 98%
Methyl 4-(chloroMethyl)benzoate, 97%
3-Methyl-2-pentene (cis- and trans- Mixture)
7-Methoxy-4-quinolinol
Cyclohexyl isocyanide
4,5,6,7-tetrahydro-5-methyl-Thiazolo[5,4-c]pyridine-2-carboxylic acid hydrochloride
a-D-Glucopyranoside,methyl 4-O-[2-O-acetyl-6-methyl-3-O-(phenylmethyl)-a-L-idopyranuronosyl]-2-deoxy-2-[[(phenylmethoxy)carbonyl]amino]-3-O-(phenylmethyl)-,6-acetate
dl-thiorphan
1-ETHYNYLCYCLOPENTANOL
AMlodipine
PotassiuM isopropoxide, 99% (Metals basis), 5% w/v in isopropanol
5-BroMo-8-fluoroquinoline
(1R)-1-[4-(Trifluoromethyl)phenyl]ethylamine 98%
5-chloro-2-hydroxybenzohydrazide(SALTDATA: FREE)
4-Nonylphenol (mixture of branched chain isomers)
3-Bromo-4-(N-morpholino)benzaldehyde
2-Chloro-N-(2,6-dimethylphenyl)acetamide
Boc-Hser(Bzl)-OH
Benzamide
3,4-Dichloroaniline
(S)-(+)-2-Phenylpropionic acid
(3-Phenyl-1,2,4-oxadiazol-5-yl)MethylaMine
Ethyl 1-(tert-Butoxycarbonyl)-3-piperidinecarboxylate
4-AMino-5-broMo-6-MethylpyriMidine
(S)-(-)-alpha-Allylalanine hydrate, 99%, 99+% ee
OsMiuM AtoMic Absorption Standard
Diethyl 1,4-Phenylenediacrylate
ethyl benzoylformate
Zinc iodide
N,N-Diethylacetoacetamide
2-Chlorocyclopentanone
OSMR ELISA Kit
PDXK ELISA Kit
TFR/CD71 ELISA Kit
Human Lebtospira ELISA Kit
Sheep IL-35 (Interleukin 35) ELISA Kit
Rat 5-Nucleotidase,5-NT ELISA Kit
INB ELISA Kit
Human flagellin ELISA Kit
PAP ELISA Kit
CYT Ab ELISA Kit
Oxford Agar Base
Eugon Broth
N-NONADECANE
Cyclohexane
5-(TrifluoroMethyl)uracil, 97%
2-Hydroxy-1,4-naphthoquinone, 98+%
imidazo[1,2-a]pyrazine
4,5-Diphenyl-4-oxazoline-2-thione
ethyl 1,3-dimethyl-1H-pyrazole-5-carboxylate
3-Mercapto-1,2-propanediol
(CYANOMETHYL)TRIPHENYLPHOSPHONIUM CHLORIDE
(-)-Epigallocatechin gallate
CyclopropylMagnesiuM BroMide
4-Benzyloxy-1-broMo-2-fluorobenzene, 98%
Bis(Methylcyclopentadienyl)niobiuM dichloride, 97%
Hexyl forMate
1-Boc-3-oxo
5-METHYL-2-NITROANILINE
Allyl acetate
Z-β-D-HoMoala-OH
AMMoniuM chloride
4,7-Diphenyl-1,10-phenanthroline, min. 97% (Bathophenanthroline)
Ethylene glycol, for analysis, 99.5%
Methacryloxypropyldimethylchlorosilane
6,8-Dimethyl-4-oxo-4H-chromene-3-carbaldehyde