SK-MES-1细胞株:人肺鳞癌细胞系培养条件 货号:GD-C8618933
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞,为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Chloro-2-Methoxypyridine-4-boronic acid, 95%
3,4-DiMethoxycinnaMonitrile, predoMinantly trans
3-Chloro-alpha-Methylbenzyl Alcohol , 98%
trans-4-(t-ButyldiMethylsilyloxy)-1-buten-1-ylboronic acid, pinacol ester
4-ISOPROPYLCYCLOHEXANONE
N-(1-Methylethyl)-benzeneMethanaMine
2-Octadecanone
N-(Chloroacetyl)-4-methylaniline
Ethyl 2-Mercaptopropionate
2-(chloromethyl)-6-methylimidazo[1,2-a]pyridine(SALTDATA: HCl)
2-bromo-N-isopropylacetamide(SALTDATA: FREE)
1-(Bromomethyl)naphthalene
3,4-Diethoxybenzoic acid
2,6-Dichloroaniline
Methyl L-histidinate dihydrochloride
2,5-Dihydroxybenzoic acid
1,1'-DinaphthylaMine
2,4-DibroMo-6-Methoxyaniline
TitaniuM(IV) oxide, 98+%, anatase powder
Periodic Acid, ACS
Bis(4-fluorophenyl) Ether
Tolylene-2,4-diisocyanate
1-(1'-Methylethyl)-4-piperidone
7-hydroxy-1,3-naphthalenedisulfonic acid, dipotassium salt
Hydroquinone Dimethyl
DICHLOROISOBUTYLMETHYLSILANE
THIAMPHENICOL
2,6-Dichlorobenzoxazole
SodiuM 1-heptanesulfonate Monohydrate, 99%
Xanthone, 99%
ANTU
Flutolanil
O-ISOBUTYLHYDROXYLAMINE HYDROCHLORIDE
Ribose
1-(2-Methoxyphenyl)-3-buten-1-ol
1,3-DIIMINOBENZ[F]ISOINDOLINE
Bromotrichloromethane
4-(Trifluoromethyl)benzenesulphonamide
1-(4-Methoxyphenyl)-1-cyclopropanecarbonitrile, 96%
4-Fluoro-3-(trifluoromethyl)phenylboronic acid pinacol ester
BROFOXINE
3,4-DINITROTOLUENE
5-BENZYLOXY-2-BROMOTOLUENE
(+)-1-(9-FLUORENYL)ETHYL CHLOROFORMATE
Hypophosphorous acid
Isobutyl 4-AMinobenzoate
3-Methyl-1h-pyrazolo[3,4-b]pyridine
3-Acetylpyridine, 98%
N-Methyl-N-(3-pyridin-3-ylbenzyl)aMine, 97%
Sucralfate
1-Methoxyphenazine
Sudan IV
(3beta,16beta,21beta,22alpha)-28-(acetyloxy)-16,22,23-trihydroxy-21-{[(2E)-2-methylbut-2-enoyl]oxy}olean-12-en-3-yl beta-D-glucopyranosiduronic acid
O-nitrophenyl myristate
Silicristin
4-Ethoxycarbonylphenylboronic acid
6-METHYL-4-(TRIFLUOROMETHYL)-2(1H)-PYRIDONE
3-Chloro-5-(trifluoroMethyl)acetophenone, 97%
1-HEPTYN-3-OL
4-Methylumbelliferyl-alpha-D-glucopyranoside
Dimethoxymethane
1-Benzoyl-3,5-bis(trifluoromethyl)pyrazole
2-(1-Methylhydrazino)quinoxaline
LEAD ZIRCONATE
Phytic acid
POTASSIUM SELENATE
Aliquat 7
3-Chloro-1,2-benzisoxazole
1,3-Cyclohexanediol, Mixture of cis and trans
Methyl 2,3,4-Tri-O-benzyl-1-thio-beta-L-fucopyranoside
2-(Pyrazol-1-yl)-3-cyanopyridine
2-AnilinopyriMidine-5-carboxylic acid, 97%
PHOSPHORUS PENTABROMIDE
1,1,1,2,2,3,3-Heptachloropropane
bis(pentane-2,4-dionato-O,O')zinc
5,6,7,8-Tetrahydroquinoxaline
Vitamin K
Asarone
HFABP ELISA Kit
BLI ELISA Kit
Human LAM ELISA Kit
Human Blocking antibody,BA ELISA Kit
IgA ELISA Kit
M2-PK ELISA Kit
Chicken 17-ketosteroids,17-KS ELISA Kit
E ELISA Kit
Human vasopeptidase inhibitors,VPI ELISA Kit
SEPP1 ELISA Kit
Sabouraud-glucose Agar with antibiotics
Egg-Yolk Manitol Salt Medium Base
Isonicotinic acid
StrontiuM zirconiuM oxide, 99.3% (Metals basis)
5-AMino-2-fluorobenzoic acid, 99%
2-Hexyn-1-ol, 97%
Tolylene-2,4-diisocyanate
Zinc iodide
2-Chloro-4,6-di(morpholin-4-yl)-1,3,5-triazine
3-Amino-1-propanol
ALLYL PHENYL SULFIDE
Trimethylsilyl trifluoromethanesulfonate
2-BroMo-4,5-diMethoxybenzonitrile, 99%
Isoquinoline-5-sulfonic acid, 95%
Ethyl 4-chloro-5,8-diMethoxyquinoline-3-carboxylate, 97%
6-α-Methylprednisolone Solution
2,4-Difluoro-3-Methylbenzonitrile, 99%
N-(Furan-2-ylMethyl) 3-broMo-5-MethylbenzenesulfonaMide
3-AMino-4-chloropyridine
4-Fluoro-5-Methoxy-2-nitroaniline
TRI-O-BENZYL-D-GLUCAL
4,4'-Diiodo-3,3'-dimethylbiphenyl
2-Bromo-5-chloronitrobenzene
(R)-2-Methylpiperidine
2-Acetamido-4-methyl-5-thiazolesulfonyl chloride, 97%