GI-1细胞株:人神经胶质瘤和肉瘤细胞系促销多 货号:GD-C8520148
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题:如不能在收到细胞后及时操作细胞,T25及离心管发货的细胞将可以消毒后在37度过夜,血清发货的细胞在室温下静置1-2天(注意不要放冰箱),干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱,若干冰完全升华,请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境,同时使部分因运输导致脱落的细胞贴壁,此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞,可以收集上清后离心(1200rpm,5min)后,将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中,由于不断震荡及环境不适,可能导致细胞破碎死亡,从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下,平衡后,贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可;悬浮细胞可以离心(1000rpm,3min)收集细胞,并用PBS重悬后再离心一次,再用新的培养基重悬并接种细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1,2,4,5-Tetrachlorobenzene, 98%
2-Methylphenyl-L-alanine
Orange oil
4-Boc-(aMinoMethyl)piperidine
1-BroMo-4-iodobenzene
VANADIUM OXIDE
Tosufloxacin tosylate
2-Amino-1,3-thiazole-5-carbonitrile 97%
N-Phenylmethanesulfonamide, 98%
2-(Piperazin-1-yl)pyridine-4-boronic acid pinacolester
(-)-Menthoxyacetyl Chloride
4-CHLOROPHENYLTHIOUREA
4-N-BUTYLBENZAMIDE
1,4,7,10,13-Pentaazatridecane
4-(1H-1,2,4-Triazol-1-yl)benzaldehyde
4-(1H-Pyrrol-1-yl)benzonitrile
Diundecyl Phthalate (Mixture of branched chain isoMers)
N,N-Diethyl 3-broMo-5-nitrobenzaMide
trans-CinnaMaldehyde, 99%
p-Nitro Blue TetrazoliuM Chloride, Crystal, Reagent
6-Hydroxy-1-naphthoic Acid
[4-amino-2-(methylsulfanyl)pyrimidin-5-yl]methanol
3-[(2-Ethylhexyl)oxy]propylamine
Dithiocarbonicaciddiethylester
2,4-Diaminopyrimidine
4-Aminobenzo-2,1,3-thiadiazole
2'-HYDROXY-1'-ACETONAPHTHONE
4-Cyanopyridine
3-(Methacryloyloxy)propyltris(triMethylsiloxy)silane, 98%
3'-Methoxyacetophenone, 97%
1-(2-Chloro-5-sulfophenyl)-3-Methyl-5-pyrazolone
Ethyl 3-broMopropionate
2-Mercapto-6-Methylthiazolo[4,5-b]pyridine
p-Tolyl isocyanate
2,2'-BIS(2-OXAZOLINE)
(R)-Tetrahydro-3-furoic acid
Betaine monohydrate, for analysis, 99+%
Pentafluorobenzyl propionate 97%
Stearanilide
Allyldimethoxysilane
1H,1H,7H-DODECAFLUOROHEPTYL IODIDE
4-Amino-1,3-benzenedisulfonic acid
1,2,3-Trichlorobenzene
Fast red RC salt;DiazoniuM salt of 4-chloro-2-aMinoanisole;Diazotized 4-chloro-o-anisidine;2-AMino-4-chloroanisole diazotated zinc double salt;Diazo red RC;Azoic diazo No.10;C.I. 37120
Water
4-Hydroxy-2-Methylquinoline
2-Phenoxybenzoic Acid
(R)-2-(1-Hydroxyethyl)pyridine, 98%
3,5-DiMethoxybenzaldehyde, 98%
Adipic dihydrazide
Tridecane
Methyl 1H-indazole-3-carboxylate
Ethyl 3-pyridazinone-6-carboxylate
S-benzoylthiamine O-monophosphate
4-Ethyl-3-thiosemicarbazide
Ethyl bromopyruvate
TRIS(ETHYLENEDIAMINE)CHROMIUM (III) CHLORIDE
1-BroMo-5-fluoro-4-iodo-2-nitrobenzene
4-Cyclopentylphenylboronic acid
Diethyl Fumarate
Diethyl (aminomethyl)phosphonate oxalate, 95%
5-(Bromoacetyl)-3-phenylisoxazole
Fmoc-Cys(Trt)-Opfp
3-Methyl-2-nitroanisole
3-BENZYLIDENE-2,4-PENTANEDIONE
6-Chloropyrimidin-4(3H)-one
3-CYANOPROPIONIC ACID METHYL ESTER
Methyl 1-Methylcyclopropyl ketone
1,4-Bis(broMoMethyl)naphthalene (contains ca. 23% isoMer)
4-Chloro-2,6-difluorophenylboronic acid
Benzyl cyanide, 99+%
5,5-DiMethyloxazolidine-2,4-dione, 99%
4-Biphenylsulfonyl Chloride
PyridiniuM TrifluoroMethanesulfonate
3,5-Bis(trifluoromethyl)-2-chloro-nitrobenzene
Methyl (2S)-(-)-2-(hydroxydiphenylmethyl)-1-pyrrolidinecarboxylate
3-BROMO-4-NITROBENZOIC ACID 97
1-Methylpyrrolidin-3-one
FGL1 ELISA Kit
Mouse Aβ42 (Amyloid Beta 42) ELISA Kit
Human EIF6 ELISA Kit
Human SCGB2A2 ELISA Kit
CT ELISA Kit
Rat PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit
Chicken Interferon β,IFN-β/IFNB ELISA Kit
Human LH ELISA Kit
Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit
Plg ELISA Kit
Reinforced Medium for Clostridia
Candida Elective Agar to Nickerson
IsochroMan
CeriuM(III) perchlorate hexahydrate, Reagent Grade
5-Chloronicotinic Acid
2-Fluoronicotinic acid, 97%
[4-amino-2-(methylsulfanyl)pyrimidin-5-yl]methanol
5-Acetoxymethyl-2-furaldehyde
Nickelous sulfate
Potassium thiocyanate
4-Aminopyrazolo[3,4-d]pyrimidine
3,5-Difluoronitrobenzene
1-Butanethiol, 98%
Methyl 3-chloropyrazine-2-carboxylate, 97+%
Diethyl diethylMalonate, 98%
2,4-Dichlorophenylboronic acid
METHYL 3,5-DIMETHYLBENZOATE
Ethyl 7-chloro-2-oxoheptanoate
5-Propyl-3-p-tolyl-1,2,4-oxadiazole
POTASSIUM TANTALATE
5-BroMo-2-piperazinopyridine, HCl
Ethyl 4-hydroxyphenylacetate
Tween 85 (=Polyoxyethylene Sorbitan Trioleate)
N-(4-Methoxybenzylidene)-4-butylaniline
9-Chlorohexadecafluorononanoic Acid