VERO C1008细胞株:非洲绿猴肾细胞系促销多 货号:GD-C8519293
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,4-Dichloro-3-ethyl-6-nitrophenol, 98%
AluMinuM acetate basic
(S)-(-)-2-BROMOPROPIONIC ACID
N-(HydroxyMethyl)acrylaMide , 98%
(11S,12S)-9,10-DIHYDRO-9,10-ETHANOANTHRACENE-11,12-DIAMINE
2-Hydroxy-2-Methylbutyric acid
5-Methyl furfural
2,5-Dichlorothiophene-3-sulfonyl chloride ,97%
Cerium(IV) trifluoromethanesulfonate, 99%
N,N-Bis-(2-chloroethyl)-p-toluenesulfonamide, tech., 90%
N-Methylmethacrylamide (stabilized with HQ)
GLUTARYL DICHLORIDE
ALL-TRANS-RETINAL
BROMOPENTAMETHYLBENZENE
Orange II
DL-PyroglutaMic acid
6,6'-DibroMo-2,2'-bipyridyl
Methyl 6-broMonicotinate
1,7,7-TriMethylbicyclo[2.2.1]heptan-2-one oxiMe, 97%
Tris(2-carboxyethyl)phosphine Hydrochloride
2-Propylcyclohexanol (cis- and trans- Mixture)
5-Fluoro-1-indanone
Acid Yellow 25
methyltrimethoxysilane
p-Hydroxycinnamic acid
(R)-1-(2-BROMOPHENYL)ETHANOL
3-METHYL-2(5H)-FURANONE
Cis-3-Hexenyl Butyrate
2-AMino-3-fluorobenzonitrile, 95%
5-AMino-2-(trifluoroMethyl)benziMidazole, 97+%
2-Methoxynaphthalene, 98%
2-BroMo-4-tert-butylphenol
EuropiuM(III) oxalate hydrate, 99.9% (REO)
N-tert-Butyl-4-MethoxybenzaMide
4-AMINOBENZOPHENONE
3-Acetyl-2-fluorophenylboronic acid
DL-PHENYLALANINOL
3-Quinuclidinone hydrochloride, 99%
2,6-Dichlorophenylacetonitrile, 97%
2-Amino-4-hydroxybenzothiazole
D-1-Cbz-Nipecotic acid
3-(2,6-Dichlorophenyl)-5-methylisoxazole-4-carboxylic acid
L-TARTARIC ACID DIBENZYL ESTER
5-BROMO-2-IODOBENZONITRILE
2,6-Dichlorobenzoyl chloride
Ethyl 2-aMino-4-phenyl-5-thiazolecarboxylate
DiphenyliodoniuM Hexafluorophosphate
1-(3-BroMophenyl)cyclopropanecarbonitrile
Silver phosphate, 98%
L-HoMocystine
DiMethyl Tetrachloroterephthalate
4-amino-3-(trifluoromethyl)benzoic acid
Methacycline Hydrochloride
5-methylbenzimidazole
2-Ethylhexyl acetate
2-PHENYLINDOLE-3-ACETONITRILE
1,4-DIBROMO-2-BUTANOL
DL-2-AMINOOCTANOIC ACID
7-BroMobenzofuran-2-carboxylic acid
Benzyl Carbazate
alpha-Angelicalactone
1,2-Bis(2,3,4,5,6-pentabromophenyl)ethane
L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt ,98%
Erlotinib hydrochloride
2-Iodopropane
p-Hydroxy-cinnamic acid
Methyl 3-isoquinolinecarboxylate
4-(Methoxycarbonyl)phenylboronic Acid
1-Butyl-3-MethyliMidazoliuM Trifluoro(trifluoroMethyl)borate
3-(3-(Ethoxycarbonyl)piperidine-1-carbonyl)phenylboronic acid
Decane, 99+%, pure
cis-1,4-Diacetoxy-2-butene, 95%
4-Nitrobenzhydrazide
2,5-Diphenyl-1,4-benzoquinone
4,4'-dimethyl-2,2'-dipyridyl
3-Bromo-6-chloro-2-pyridinecarboxylic acid
t-Butyl(E)-3,5-dihydroxy-7-[3'-(4''-fluorophenyl)-1'-methylethyl-indol-2'-yl]-6-heptenoate
rosarin
EAR12 ELISA Kit
P-GP ELISA Kit
Human PRM2 ELISA Kit
Human DHODH ELISA Kit
1,3-βD glucosidase ELISA Kit
Rat β-CTx (Beta Crosslaps) ELISA Kit
Chicken Immunoglobulin G,IgG ELISA Kit
Human PCI (Protein C Inhibitor ) ELISA Kit
Human 50% complement hemolysis,CH50 ELISA Kit
FFA ELISA Kit
Andrade′s Carbohydrate Broth Base
Power-nitrate Medium
LutetiuM(III) 2,4-pentanedionate, REacton|r, 99.9% (REO)
CalciuM fluoride, 99%
5-(Chlorosulfonyl)salicylic acid, 97%
2-(Dicyclohexylphosphino)-2,4,6-Triisopropylbiphenyl
5-Fluoro-1-indanone
Deflazacort
Fluorographite polymer
hexacosanoic acid
Tetraisopropyl methylenediphosphonate
SODIUM 4-N-OCTYLBENZENESULFONATE
1-BroMo-5-Fluoropentane
HoMoMorpholine hydrochloride, 98%
^b-Naphthoflavone, 98+%
EDTA dipotassiuM salt
3-Ethylaniline
1-BroMo-3-fluoro-5-pyrrolidinobenzene
2-BroMoMethyl-4-fluorophenylboronic acid, neopentyl glycol ester
Farnesal, Mixture of isoMers
N-(2,6-DiMethylphenyl) 2-broMobenzaMide
Conduritol B epoxide
4-Bromo-2-methoxypyrimidine
2,4-Difluorobenzophenone
4-Amino-2-chloro-5-fluoropyrimidine, 98%