HO-8910细胞株：人卵巢癌细胞系权威供应 货号：GD-C8319031
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-AMinothiazole, 97%
(4-Fluorophenyl)acetone
5-AMino-4-cyano-1-(2,5-dichlorophenyl)-3-Methylpyrazole
2,4,6-Trifluorobenzonitrile
2-(4-tert-Butylphenyl)-5-(4-biphenyl)-1,3,4-oxadiazole
2-Hydroxybenzaldehyde phenylhydrazone
4-Ethylbenzoyl chloride
2-Chloro-4-fluorobenzyl bromide
Methyl 4-[2-nitro-4-(trifluoromethyl)anilino]-butanoate
1-(2-Mesitylene)-1,3-butanedione
Isothiazole-5-carboxylic acid methyl ester
5,5-DIMETHYL-1,3-DIOXAN-2-ONE
CHLORPHENESIN CARBAMATE
1,3-Bis(bromomethyl)benzene
1-(BroMoMethyl)-2-(phenoxyMethyl)benzene
3-(5,5-DiMethyl-1,3,2-dioxaborinan-2-yl)pyridine
2',4'-Dichlorovalerophenone
Ethyl 8-broMo-4-hydroxy-6-(trifluoroMethoxy)quinoline-3-carboxylate
Triethyl orthoacetate, 97%
PotassiuM Iodide, USP
Ethyl 2-Undecynoate
Ethyl 2-methylacetoacetate
5-methylhex-4-enoic acid
2,6-diethylpyridine
3,4-Dihydroxycinnamic acid
METHYL PHENYLPROPIOLATE
4-N-BUTYLOXYBIPHENYL
1-Methylindazole-3-Carboxylic Acid
4-Methyl-1,2,3-thiadiazole-5-Methanol, 99%
2-BroMopropionyl broMide, 97%
Zinc peroxide
2-BroMo-5-chlorobenzotrifluoride
4-Cyano-3-Methoxyphenylboronic acid
PotassiuM P,P-diMethylphosphonoacetate
N-Boc-O-Benzyl-D-serine
Ethynyl estradiol
Tantalum(V) oxide, 99.8% (99.95+%-Ta)
4-Bromo-3-fluorobenzotrifluoride
2,6-Diacetoxynaphthalene
1,1,3-Trimethoxypropane, 98%
9-Vinylcarbazole
2-ACETOXYETHYLTRICHLOROSILANE
4,4'-Dithiodianiline
3-Methylpentane;1,1-Diethylethane
2-AMino-5-broMopyrazine
FMoc-β-(3-pyridyl)-Ala-OH
4-Octadecyloxybenzaldehyde
4-Chloro-6-Methoxyquinolin-7-ol
2-BroMo-1-(4-chloro-3-nitrophenyl)ethan-1-one, 95%
Ammonium bisulfite
6-Methyl-2-thiouracil
2,9-diphenyl-1,10-phenanthroline
pigment orange 62
2-Methylindan-1-one
Citronella oil
4-Fluorobenzylamine
Monoethyl fuMarate
N-FMoc-L-alanine Monohydrate, 98%
2-BroMo-6-ethoxypyridine
3,4-Methylenedioxybenzylamine ,98%
Ethyl 4-nitrobenzoylacetate, 98%
5-Acetyl-2-amino-4-methylpyrimidine
5-(Hydroxymethyl)-4-methyl-1,2,3-thiadiazole
2-Thienylglyoxal
Pentadecafluorooctanoyl chloride
CROTONALDEHYDE
2,4-Dimethyl-3-pentanone
TetraethylaMMoniuM hydrogensulfate
1,4-Phenyldiboronic acid
7,7-Dichlorobicyclo[4.1.0]heptane
3,6-Dichloro-2-pyridinecarboxaldehyde
4'-Methyl[1,1'-biphenyl]-4-sulfonyl chloride, 97%
1,1-DICHLOROPROPENE
4-Nitrobenzoylglycylglycine
3-bromo-4-chloroaniline
2-Bromopropionyl chloride
Tropicamide
Isoflavone
CYP21A ELISA Kit
Mouse α-HydroxybutyRate Dehydrogenase,αHBDH ELISA Kit
MPS ELISA Kit
Human Cpn ELISA Kit
RANK ELISA Kit
S100B ELISA Kit
Chicken Interleukin 18,IL-18 ELISA Kit
CRF ELISA Kit
Human Vascular Endothelial-Cadherin Complex,VE-cad ELISA Kit
CLS ELISA Kit
Bacteroid Selective Agar
Minimal Dextrase Medium
Isoxazole
Bacitracin
5-Chloropyridine-3-boronic acid, 95%
2-Fluorobenzoyl chloride, 97%
Ethyl 2-methylacetoacetate
ethyl (R)-(-)-mandelate
Strontium bromide hexahydrate
3-bromo-1,1,1-trifluoro-2-butanone
1-Bromo-5-chloropentane
Tetraisopropyl methylenediphosphonate
2-Mercaptothiazole
ZirconiuM dichloride oxide hydrate, Puratronic|r, 99.9985% (Metals basis)
2-Methyl-5-nitrophenol, 97%
2-Iodo-5-Methylthiophene
CalciuM stearate
NiobiuM carbide
1-Isobutylpyrazole-4-boronic acid, pinacol ester
2,4-DIAMINOANISOLE DIHYDROCHLORIDE
1-ETHYL-2,3-DIMETHYLIMIDAZOLIUM BIS(TRIFLUOROMETHANESULFONYL)IMIDE
(+)-N,N,N',N'-Tetramethyl-L-tartardiamide
Methacryloxymethyltrimethoxysilaneinhibited with MEHQ
5-Chloropyridin-3-ol, 97%
4-Chloro-3-(ethoxycarbonyl)benzeneboronic acid 96%
