GNM细胞株：人上颌牙龈癌颈淋巴结转移癌细胞系体外培养

GNM细胞株：人上颌牙龈癌颈淋巴结转移癌细胞系体外培养 货号：GD-C8219587
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(S)-(+)-1-(2-PyrrolidinylMethyl)pyrrolidine
SulfluraMid
4-ForMylbenzoic acid
2-(DODECYLOXY)ETHANOL
3,5-DIMETHYLBENZENESULFONYL CHLORIDE
4H-PYRAN-4-ONE
Tetrapropylammonium hydroxide, 25% in water
2-Fluoro-4-(trifluoromethyl)benzoyl chloride, 97%
(S)-(-)-alpha-Methylbenzyl isocyanate, 98%
Recombinant Isocitrate Dehydrogenase
1H,1H,10H,10H-PERFLUORO-1,10-DECANEDIOL
3-Amino-4-methoxybenzamide
3-IODOPHENYLACETONITRILE
ETHYL NONAFLUOROBUTYL ETHER
2-Chloro-1,3-thiazole-5-carbonyl chloride
1-(3-BroMophenyl)piperidine
(1-Ethylpropyl)benzene
t-Butyl 4-iodopyrazole-1-carboxylate
2-BroMo-1-(2,3-dihydro-1,4-benzodioxin-6-yl)ethan-1-one, 95%
ALDICARB SULFOXIDE
Methyl 4-BroMo-3-hydroxybenzoate
n-Undecane
Sodium Chlorate
2-HYDROXYADENOSINE
2-amino-3-nitro phenol
2,4-DIMETHYL-3-HEPTANOL
Ethyl 3-(N,N-dimethylamino)acrylate
Trans-4-Cyclohexyl-L-Proline
BariuM hydroxide, anhydrous, 94-98%
3-O-Benzyl-4,6-O-isopropylidene-D-glucal, 97%
3,4-Dihydro-2H-pyran
Growth HorMone Releasing Factor bovine
Heptadecafluoro-1-octanesulfonyl chloride
Nalpha-FMOC-L-GlutaMine
BETA-D-GLUCOSE (CONTAINS ALPHA-D-GLUCOSE)
3-Methyl-2-nitrophenol
2-Imidazolidone, anhydrous, 96%
2-Nitro-p-cymene
L-4-Chlorophenylglycine
4,4'-Azobis(4-cyanovaleric Acid) (contains ca. 20% Water)
4-Chloro-2-hydroxy-3-nitropyridine
Ondansetron hydrochloride
BENZYL ACETOACETATE
HoMovanillic acid;4-Hydroxy-3-Methoxy-α-toluic acid;4-Hydroxy-3-Methoxyphenylacetic acid;HoMoprotocatechuic acid 3-Methyl ether;HVA
2,2-DiMethyl-6-chroManesulfonyl chloride
Isopropyl isocyanate
4-Pyridineethanethiol Hydrochloride
(S)-1-(4-Chlorophenyl)ethylaMine, 95+%, (suM of isoMers), 95% e.e.
3-(4,4,5,5-TetraMethyl-1,3,2-dioxaborolan-2-yl)pyridine, 97%
16BETA-METHYL-16ALPHA,17ALPHA-EPOXYPREGNENOLONE
N-SucciniMidyl Acrylate
2-bromo-1-(2-methylphenyl)ethanone
3-phenyl-2-(phenylsulfonyl)oxaziridine
hexamethylenediamine-N,N,N',N'-tetrakis-(methylphosphon.ac.)
barium perchlorate
N-(N-PROPYL)ACETAMIDE
Poly(vinyl alcohol)
2,4-BIS(TRIFLUOROMETHYL)BENZONITRILE
1,4-Dihydro-2-Methylbenzoic acid
1,5-Dimethyl-1H-pyrazole-3-carboxylic acid ,97%
4-(4-Chlorophenoxy)phenylhydrazine hydrochloride
sec-Butyl Ethyl Ether
Terephthalic-d4 acid, 97 atom% D, for NMR
2-Bromo-6-methoxypyridine
Tris(2-chloroethyl) phosphate
L-Arginine L-glutamate
Octacosane
SodiuM chlorite
2-(Methylthio)phenol
Pentyl Valerate
1,2-Diiodobenzene, 98%
5-Fluoro-2-Methoxyphenylboronic acid, 98%
N-Methyl pyrrole
3,4-Hexanedione
Zirconium oxysulfate
4-Hydroxy-6-Methylnicotinic Acid
Tris(1-chloro-2-propyl) phosphate
3-methylcinnamic acid
GPI ELISA Kit
CD40L ELISA Kit
Human REG4 ELISA Kit
Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) ELISA Kit
Albumin ELISA Kit
Rat soluble interleukin-2 receptor,IL-2sRβ ELISA Kit
Nude Mouse cyclooxygenase-2,COX-2 ELISA Kit
Human βAPP ELISA Kit
Human oxidized lowdensity lipoprotein，OxLDL ELISA Kit
NUP50 ELISA Kit
Rappaport-Vassiliadis R10 broth
Salmonella Shigiella Enrichment Broth
Ethylurea
TRANS-1,2-DICHLOROCYCLOHEXANE
4-Ethoxy-1,1,1-trifluoro-3-buten-2-one, 94%, stab.
2-AMinoethyl diphenylborinate, 98%
n-Undecane
dimethyl 1,2,2-trimethylcyclopentane-1,3-dicarboxylate
N-alpha-(tert-Butoxycarbonyl)-L-lysine
3-Iodobenzoic acid

2-(2-PYRIDYL)BENZIMIDAZOLE
2-(4-Bromomethyl)phenylpropionic acid
Ethyl 3-(2-furyl)propionate, 98%
Pentafluorobenzoyl chloride, 98%
2-Chloro-5-iodopyridine, 98%
L-Citrulline
5-AMINOBENZOTRIAZOLE
(-)-2,2'-METHYLENEBIS[(3AS,8AR)-3A,8A-DIHYDRO-8H-INDENO[1,2-D]OXAZOLE]
2,6-Dichloronitrobenzene
N-Ethyl 3-broMo-5-MethylbenzenesulfonaMide
2-PYRIDYLTHIOUREA
4-Hydroxy-3-methoxybenzoic Acid Sodium Salt
2-Acetylthiazole
Tetrakis(2,2,6,6-tetramethyl-3,5-heptanedionato)niobium(IV), 99% [Nb(TMHD)4]
2,2,4,4-Tetramethylpentane