BRL 3A细胞株：大鼠肝细胞系传代培养

BRL 3A细胞株：大鼠肝细胞系传代培养 货号：GD-C8119218

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-Nitro-M-xylene, 99%
Arg-Phe acetate salt
2-AMino-4,5-diMethoxybenzonitrile
1,6-Diisocyanohexane
2-N-PROPYLTHIAZOLE
ALLYL N-PROPYL SULFIDE
4-METHYL-2-PYRROLECARBOXYLIC ACID ETHYL ESTER
6-Iodo-pyridine-2-carboxylic acid methyl ester
4-(Hydroxymethyl)cyclohexanecarboxylic Acid (cis- and trans- mixture)
Ethyl 1,2,3-thiadiazole-5-carboxylate
Ketoconazole
2-Chlorobenzene-1,4-diammonium sulphate
5-FLUORO-4-HYDROXYQUINAZOLINE
2-CHLOROBENZENESULFONYL ISOCYANATE
2-BroMo-5-nitropyridine
Silver nitrate
(1R,2R)-N,N'-DiMethyl-N,N'-bis(3,3-diMethylbutyl)cyclohexane-1,2-diaMine
Methyl 5-broMo-4-chloro-8-Methylquinoline-2-carboxylate
VitaMin D2, 99%
SodiuM Thiosulfate, Pentahydrate, Reagent, ACS
Dihydrogen Di-Mu-chlorotetrakis(di-tert-butylphosphinito)dipalladate
Valeryl chloride
N-chloroacetylglycine
4-(2-pyridylazo)resorcinol
4-amino-3-fluorobenzoic acid
(R)-(-)-2-AMINO-6-PHOSPHONOHEXANOIC ACID HYDRATE
Methyl Green zinc chloride salt
D-Alaninol
2-BroMo-5-fluorobenzyl broMide, 97%
2-Chloro-2,2-difluoroacetaMide, 98%
1-(tert-ButyldiMethylsilyl)indole-5-boronic acid, 97%
3-(Boc-aMinoMethyl)azetidine hydrochloride
DiMethyl 3,3'-dithiobispropionate
PalladiuM(II)-aMMoniuM chloride
Chlorodicarbonyl(1,2,3,4,5-pentaphenylcyclopentadienyl)rutheniuM(II)
4-DIMETHYLAMINO-1-NAPHTHYL ISOTHIOCYANATE
1-(3-Methoxyphenyl)-1-heptanone
Calcium phosphate, dibasic (99.95%-Ca)
Acid Blue 20
3-Bromo-N-butylbenzenesulphonamide 98%
2,6-Dichloro-4-(piperidin-1-ylcarbonyl)pyridine
2-Picoline
1-Bromo-3,4,5-trimethoxybenzene
Calcium hydrogenphosphate dihydrate
Benzamidine hydrochloride
1-BroMo-2,6-dichlorobenzene
6-DiMethylaMino-1-hexanol
3-BroMo-4-Methoxyphenyl Mesylate
PotassiuM broMate, ACS reagent
Fullers Earth, Powder
2-PhenylethylaMine
n-Butyl Benzoate
Direct Yellow 4
Anethole Trithione
2,3-Dichloro-1-propene
2,6-Difluorobenzoic acid
3'-AMino-4'-Methoxyacetanilide
1-(6-CHLOROPYRIDAZIN-3-YL)PIPERIDINE-4-CARBOXAMIDE
Luteolin
4-Fluorobenzophenone
5-Bromo-2-methoxycinnamic acid
Lead antimonide, 99+%
5-Chloro-3-methyl-1-p-tolyl-1H-pyrazole-4-carbaldehyde
5-Aminonicotinic acid
C13-C16 Isoparaffin
BOC-D-GLU-OH
4-Nitrophenyl-beta-D-galactopyranoside
tert-butyl 4-[4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl) phenyl]-1-carboxylate
1-BroMo-2-fluoroethane
2,2,3,4,4,4-Hexafluoro-1-butanol
5-Fluoro-2-Mercaptobenzothiazole
2,5-DiMethyl-1,3-oxazole-4-carbonyl chloride, 97%
Z-VAL-ALA-OME
3-Mercaptopropionic Acid
m-methylcinnamic acid
LEAD SALICYLATE
1-phenylpiperidine
tylosin
CML ELISA Kit
Mouse Slit2 ELISA Kit
FBN1 ELISA Kit
Human ubiquitin D,UBD ELISA Kit
Chicken NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
a-Glu ELISA Kit
Chicken very low density lipoprotein,VLDL ELISA Kit
PC/CPG ELISA Kit
HO2 ELISA Kit
IGFBP1 ELISA Kit
Wheat Bran Medium
Strain Medium
Ethyl acetoacetate
Zinc fluoride tetrahydrate, 98%
5,6-DiMethoxy-1-indanone, 97%
2-Fluoro-4-hydroxybenzeneboronic acid pinacol ester, 96%
Valeryl chloride
Ammonium iron(III) oxalate hydrate
Methamidophos E.C.
Benzenediazonium, 3-bromo-, tetrafluoroborate(1-)

Guaiacol carbonate
TRIPHENYLMETHYL MERCAPTAN
2-Propanethiol
Iron(III) fluoride trihydrate
(3-Benzyloxypropyl)triphenylphosphoniuM broMide, 98%
2-BroMo-6-fluorobenzonitrile
StrontiuM nitrate
4-(N,N-DiMethylsulfaMoyl)-2-Methylphenylboronic acid
5-Butoxy-4-chloro-2-fluorophenylboronic acid, pinacol ester
3,4-DIHYDRO-2H-1,5-BENZODIOXEPINE-7-CARBOXYLIC ACID
4-tert-Butylphthalic anhydride
Berberine Sulfate Hydrate
5-Bromo-4-methylpyrimidine
Pyrazolo[1,5-a]pyridine-3-carboxylic acid , 97%
Dilithium tetrabromonickelate(II), 0.5M solution in THF, AcroSeal