COLO-320细胞株：人结肠腺癌细胞系传代培养

COLO-320细胞株：人结肠腺癌细胞系传代培养 货号：GD-C8119141

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-tert-Butylcatechol, 98%
Ethyl acetoacetate
2-Methyl-5-nitrophenylboronic acid, pinacol ester
5-CHLOROBENZOXAZOLE
Caffeic acid
2-(TRIFLUOROMETHOXY)BENZONITRILE
1-Methylindazole-7-boronic acid
2,5-Dichloroanisole, 99%
Tin(II) sulfate, 95+%
5-Chloro-2-methylphenol
1-(4-Benzyloxyphenyl) hydrochloride
4-PENTEN-1-YL ACETATE
3-AMINO-2-CHLOROPYRIDINE
2-ACETAMIDO-5-CHLOROTHIAZOLE
5-BroMo-2-Methyl-4-nitro-1H-iMidazole
Methyl 3-(chloroforMyl)benzoate
4,7-DibroMo-3-hydroxy-2-naphthoic Acid
Vinyltoluene MonoMer (M- and p- Mixture) (stabilized with TBC)
Tris(dibenzylideneacetone)dipalladiuM-chloroforM adduct, 97%
Silver Protein, Strong
6,7-DihydroxycouMarin-3-carboxylic Acid
1-Chloro-4-ethylbenzene
Vat Black 29
2,2,4,4-Tetramethylpentane
2-Bromo-5-Fluorobenzoic Acid
2-[(2-Methylphenoxy)methyl]oxirane
Glyoxylic Acid Monohydrate
2-(2-Chloroacetyl)-6,7-diMethoxy-1,2,3,4-tetrahydroisoquinoline, 96%
Diacetone acrylaMide, 99%
AMMoniuM iron(II) sulfate hexahydrate, 99%

4-PHENYLTETRAHYDRO-2H-PYRAN-4-CARBOXALDEHYDE
N-Acetyl-DL-Methionine
Phenyl sulfoxide
4-Cyano-4'-ethylbiphenyl
4-AMinoacetophenone
Iron(III) citrate hydrate, 98%
4-Amino-1H-indazole, N1-BOC protected
5-(4-Fluorophenyl)-picolinic acid
6-methyoxy-2(3H)-benzothiazolone
Nickelous oxide
2,6-Pyridinedimethanol
FMOC-TYR(PO(OBZL)OH)-OH
4-Aminotoluene-3-sulfonic acid
SodiuM tetraethylborate
N-Carbobenzoxy-DL-phenylalanine
5-(Methoxycarbonyl)thiophene-3-boronic acid
Ethyl 3-chlorophenylacetate, 98+%
4-PyriMidin-5-ylbenzoic acid, 97%
3,9-Bis[2-(3,5-diaMino-2,4,6-triazaphenyl)ethyl]-2,4,8,10-tetraoxaspiro[5.5]undecane
N-ButylethylenediaMine
2,6-Dichloroquinoline
thiamine
3-(Bromomethyl)-2-cyclopropyl-4-(4'-fluorophenyl)quinoline
3-methyloxetane-3-carbaldehyde
FLUOROPHOSPHORIC ACID
5-BroMo-2-fluorophenylboronic acid, pinacol ester
Tropolone
5-AMino-o-cresol
3,4,5-Trimethoxycinnamic Acid
7-[3-(Trifluoromethyl)phenyl]-4,5-dihydropyrazolo[1,5-a]pyrimidine-3-carboxamide, 90%
Phenylacetonitrile
2,7-Diaminofluorene
5,7-Dihydroxy-4-methylcoumarin
2,3,6-Trichloropyridine
4-FLUORO-3-METHYLANISOLE
4-Epianhydrotetracycline Hydrochloride
2-BroMobiphenyl
1-AMinocyclobutanecarboxylic Acid Hydrochloride
(Z)-3-Cyclopropylbut-2-enoic acid
4-Chloro-1-naphthol, 97%
3-(1H-IMidazol-1-ylMethyl)aniline, 97%
Butyraldehyde
5-Nitroanthranilic Acid
hexetidine
(1R,4S)-N-BOC-1-Aminocyclopent-2-ene-4-carboxylic acid
1-(carbobenzyloxy)-2-piperidinecarboxylic acid
2-hydroxymethyl-6-methoxy-1,4-benzoquinone
MT ELISA Kit
HRAS ELISA Kit
Human APOC4 ELISA Kit
Human DEFβ119 ELISA Kit
Chicken Avian Leukosis Virus AG,ALV-AG ELISA Kit
COL6α1 ELISA Kit
CH ELISA Kit
Human ZAN ELISA Kit
Human angiopoietin-like protein 3,ANGPTL3 ELISA Kit
Guinea pig 50% complement hemolysis,CH50 ELISA Kit
Ureaplasma urealyticum Agar Base
Sensitivity Disk Agar
GerManiuM(IV) isopropoxide, 99.9% (Metals basis)
Ferrous fumarate
2,3-DiaMino-5-chloropyridine, 98+%
2-Fluoro-5-broMobenzyl broMide
1-Chloro-4-ethylbenzene
Solvent Yellow 79
Dimethyl silicone oil 201
(1S)-1-[3-(trifluoromethyl)phenyl]ethanol

Glyoxylic acid monohydrate
2,6-Difluoroiodobenzene
BariuM broMide, ultra dry, 99.998% (Metals basis)
(S)-4-AMino-2-(Boc-aMino)butyric acid, 97%
Bis(pentaMethylcyclopentadienyl)nickel
Crotyl alcohol, Mixture of cis and trans
2,2-DIMETHYL-N-[3-(4,4,5,5-TETRAMETHYL-[1,3,2]DIOXABOROLAN-2-YL)-PYRIDIN-2-YL]-PROPIONAMIDE
5-(5-Chloro-2-hydroxy-4-Methylphenyl)-1-phenylpyrazole
7-BroMo-1-heptanol
(4S,5S)-(+)-2,2-DiMethyl-5-MethylaMino-4-phenyl-1,3-dioxane
3-Fluoro-5-(hydrazinecarbonyl)phenylboronic acid
4-Bromo-6-(trifluoromethyl)-1H-benzimidazole 97%
1,1,1',1'-Tetramethyl-4,4'-(methylenedi-p-phenylene)disemicarbazide
1-Bromo-2-ethylbutane (stabilized with Copper chip)
4-(Dimethylamino)phenyldiphenyl phosphine