COLO 320DM细胞株：人结直肠腺癌细胞系传代培养

COLO 320DM细胞株：人结直肠腺癌细胞系传代培养 货号：GD-C8119095

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-Tritylphenol, 98%
Galangin
1-(2-CHLOROETHYL)NAPHTHALENE
2,4-Di(tert-butoxy)pyriMidin-5-ylboronic acid hydrate
TripotassiuM hexachlororhodate
(S)-1-{(RP)-2-[Di(1-naphthyl)phosphino]ferrocenyl}ethyldi(3,5-xylyl)phosphine
DI-N-BUTYLTIN SULFIDE
4-Hydroxybenzoic acid, 99+%
3-(4-Methoxyphenyl)-5-isoxazolecarboxylic acid
(1-ethylpyrrolidin-3-yl)methanol(SALTDATA: FREE)
1,1?Bis((2S,5S)-2,5-di-i-propylphospholano)ferrocene, min. 97%
Benzyltrimethylammonium tribromide
5-(4-METHYLPHENYL)-1,3,4-OXADIAZOLE-2-THIOL
trans-1-Cinnamyl
(-)-CaMphanic acid chloride
BenzyltriethylaMMoniuM broMide
2,4-Dichlorothiazole
4-Methoxycarbonyl-2,3-dihydro-1h-indole
Tri-n-hexylaluMiniuM, 0.4M solution in hexane, AcroSeal
Quinaldine Red
3-Heptylthiophene
3-Nitro-L-tyrosine
4-Ethylbiphenyl
ethyl 5-nitro-2-furoate
Chlorodifluoroacetophenone
2,4-DICHLOROPHENYL ISOTHIOCYANATE
2,3-DIHYDROXY-6,7-DIMETHYLQUINOXALINE
Silver sulfate
N-[4-BroMo-2-(trifluoroMethyl)phenyl]thiourea, 98+%
1-Iodo-3-nitrobenzene, 99%
2-Picoline borane coMplex
2-Chloropyridine N-oxide hydrochloride
CalciuM sulfate dihydrate
PipeMidic Acid Trihydrate, 98%
4-(N-MethylaMinocarbonyl)phenylboronic acid, pinacol ester
2-BroMo-3-Methoxy-6-nitropyridine
3,4-Dichlorobenzaldehyde oxime, 97%
Terephthalic Dihydrazide
Palmitic Acid Vinyl Ester (stabilized with MEHQ)
L-Alanyl-L-proline
4,4'-Bis(diethylamino) benzophenone
1,4-PHENYLENE DIISOTHIOCYANATE
S-ADENOSYL-L-METHIONINE
Hydroxyethyl starch
2-BroMo-6-pyrrolidin-1-ylpyridine
DisodiuM Adipate
3-Phenyl-pyridin-2-ylaMine
3-BroMobenzylaMine hydrochloride, 99%
1-Thio-beta-D-glucose, sodiuM salt hydrate, 99%
PLATINUM(IV) SULFIDE
3-Glycidyloxypropyl(diMethoxy)Methylsilane
2-Phenylpropionaldehyde
Itopride
4-Ethoxy-3-methoxybenzyl alcohol
1-Bromo-5-methylhexane
4-BROMO-2-ETHYLANILINE HYDROCHLORIDE
2-Anilinophenylacetic acid
4-BroMo-2,2-difluoro-1,3-benzodioxole
1-PIPERIDINEPENTANOL
Beryllium powder (99+%)
1-Methyl-3-(trifluoromethyl)-1H-pyrazole-4-sulfonyl chloride, 97%
(R)-(-)-(3,5-Dioxa-4-phospha-cyclohepta[2,1-a;3,4-a']dinaphthalen-4-yl)dimethylamine, min. 97% (R)-MONOPHOS
Perfluorooctanoyl chloride
D(+)-Glucose
FMOC-(S)-3-AMINO-4-(4-IODO-PHENYL)-BUTYRIC ACID
Magnesium sulfate
DISPERSE BLUE 1
Hydrogen hexachloroplatinate(IV) hydrate
18-BroMo-1-octadecene
(3-Cyanophenyl)boronic acid, neopentyl glycol ester
Choline chloride, 99%
2-Benzoylthiophene, 98%
Nile Red
N-Tris(hydroxyMethyl)Methyl-2-aMinoethanesulfonic Acid [Good's buffer coMponent for biological research]
O-tolylmagnesium bromide
12-(trichlorosilyl)dodecanenitrile
5-Bromo-N-{[(2-methyl-2-propanyl)oxy]carbonyl}tryptophan
(1alpha,2alpha,4alpha)-1,2,4-Cyclohexanetricarboxylic acid
CpG-ODN ELISA Kit
Pre-α2 MG ELISA Kit
Human EBF2 ELISA Kit
Human MCT ELISA Kit
Chicken Luteinizing Hormone,LH ELISA Kit
TIEG1 ELISA Kit
DEV ELISA Kit
Human PRL ELISA Kit
Human Vascuoar endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA Kit
ATA ELISA Kit
Murashige & Skoog Macronutrients
Anaerobic Agar Base
LithiuM tantaluM isopropoxide, 99.9% (Metals basis)
H-Gly-Otbu.Hcl
5-Chloro-2-hydroxy-3-nitropyridine
2-Fluoro-D-phenylalanine, 99+% (suM of enantioMers)
3-Nitro-L-tyrosine
Reactive Orange 16
inulin
o-fluorostyrene

1-(4-Bromophenyl)octane
TRIETHANOLAMINE LAURYL SULFATE
Isopropyl BroMide
LithiuM hexafluorophosphate, 98%
2-(4-Methyl-5-thiazolyl)ethyl acetate, 98+%
4-Hydroxy-1-indanone
p-Fluoro benzaldehyde
2-OCTYNOIC ACID
SODIUM 1-UNDECANESULFONATE
5-AMinooxindole
2-Hexyne, 98+%
5-Fluoro-2-(trifluoromethyl)benzaldehyde 97%
5-(3,4-Dichloro-phenyl)-1 H -pyrazole-3-carboxylic acid
[4-(5-methyl-1H-tetrazol-1-yl)phenyl]acetic acid(SALTDATA: FREE)
2-Amino-3-bromobenzotrifluoride 98%