COLO-1细胞株：人角质形成细胞系传代培养

COLO-1细胞株：人角质形成细胞系传代培养 货号：GD-C8118795

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Diphenyl sulfoxide, 98+%
HeptaMethyldisilazane
4-BroMo-1-t-butyl-3-Methylpyrazole
TERT-BUTYL 2-[1-(4-CHLOROPHENYL)-2-IODO-1-METHYLETHOXY]ETHYLCARBAMATE
DECAMETHONIUM IODIDE
(?)-Epiglobulol
2-Methoxyquinoline-3-boronic acid
Pancreatin, powder, for biochemistry
Chrysin
2,3',6-Trichloroindophenol sodium salt, pract.
1-Butyltetrahydro-4(1H)-pyridinone
3-Chloro-2,4-difluorobenzoic acid
2-Bromobenzonitrile
3,4-DIMETHYLBENZOTRIFLUORIDE
3,4-Dihydroxy-3-cyclobutene-1,2-dione
4-AMino-2-fluorophenol
Dibutyldichlorosilane
5-BroMo-2-(trifluoroMethoxy)cinnaMic acid
TetrabroMophenolphthalein ethyl ester, potassiuM salt, pure, indicator grade
N,N-DiMethylacetaMide, HPLC Grade
3-Buten-1-ol
Oxamic hydrazide
Azidotrimethylsilane
HFC-236cb
Tropine
CADMIUM PHOSPHIDE
2-Isonitrosoacetophenone
4-Methoxy-1-butanol, 98+%
2-Chloro-N-[2-(diethylaMino)ethyl]benzaMide, 97%
AMMoniuM Molybdate (di), 10% w/v aq. soln.
SodiuM chroMate tetrahydrate
2,6-Difluorophenylboronic acid
N-BOC-(METHYLAMINO)ACETALDEHYDE
SodiuM pyruvate
3-(1H-Tetrazol-5-yl)benzoic acid, 97%
Basic Red 2
Methylenebisphosphonic acid
Benzonitrile, for spectroscopy, 99+%
Diethyl 3,4-furandicarboxylate, 95%
Methyl 2,5-Dihydrothiophene-3-carboxylate
1,3-BIS(3-AMINOPROPYL)TETRAMETHYLDISILOXANE
(+)-Taddol
5-AMINOTETRAZOLE MONOHYDRATE
Di-n-octyltin oxide
3-Chlorobenzyl cyanide
Titan Yellow
4-Methoxycarbonyl-3,5-diMethylphenylboronic acid
Ethyl (2R)-2,3-epoxypropanoate, 97%
(tert-ButyldiMethylsilyl)acetylene, 97%
2-Hydroxy-4-(trifluoroMethyl)pyriMidine
1,2-DiphenylethylaMine
Dibutylurea
Promethazine hydrochlorine
azelastine hydrochloride
(s)-2-methyl-2,4-pentanediol
4,5-Dimethylthiazole
1-BroMo-5-iodo-4-nitro-2-(trifluoroMethoxy)benzene
1-BOC-4-broMo-indole-2-boronic acid
2-(AMINOMETHYL)-5-METHYLPYRAZINE
p-Xylylene Dicyanide
Geranyl Formate [for Perfumery]
4-Chloro-1-methylpiperidine
3-Phenyl-benzo[c]isoxazole-5-carboxylic acid
3-tert-Butoxy-3-oxopropanoic acid
2,4-Dichloropyrimidine
3-Aminobenzonitrile
DL-Pipecolinic acid
N,N,N',N'-TetraMethyl-O-(N-succiniMidyl)uroniuM tetrafluoroborate
1,2,3,4,5,6-Cyclohexanehexacarboxylic Acid Monohydrate
Phenyl-d5-boronic acid
HEPES sodiuM salt, 99+%, for Molecular biology, DNAse, RNAse and Protease free
alpha-Fluorophenylacetic acid, 90%
2',3',5'-Tri-O-acetyluridine
(R)-(-)-2,2-DiMethyl-1,3-dioxolan-4-ylMethyl p-Toluenesulfonate
4-methoxyazobenzene
o-Xylene
4-bromo-2-chloro-5-fluorobenzoic acid
2-Aminophenol-4-(2'-methoxy)sulfonethylamide HCl
KLKB1 ELISA Kit
Mouse Interleukin 13,IL-13 ELISA Kit
Human ITIH4 (Inter Alpha-Globulin Inhibitor H4) ELISA Kit
CAMK2 ELISA Kit
Chicken IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit
DKK2 ELISA Kit
COR ELISA Kit
PSA U S ELISA Kit
Human Mothers against decapentaplegic homolog 1,Smad1 ELISA Kit
DAP6 ELISA Kit
Ferric Nitrate Agar
Enterobacteria Enrichment Broth Mossel
Calcium stearate
Iron silicide, 99.9% (Metals basis)
5-Butyloxazolidine-2,4-dione
2-Methyl-N-(3-oxetanylidene)propane-2-sulfinaMide, 95%
Oxamic hydrazide
Diastase
DL-4-Hydroxymandelic acid
Di-N-pentyl phthalate

2-AMINOPERIMIDINE HYDROBROMIDE
4-TERT-BUTYLANISOLE
4-BroMo-2-Fluorobenzyl Alcohol
Methyl 2-acetaMido-5-broMobenzoate, 97%
Carbohydrazide, 97%
3-Fluoro-2-Methylbenzoic acid
2,4-Difluorobenzyl BroMide
8-BroMo-6-MethyliMidazo[1,2-a]pyridine-3-carbaldehyde
TRANS-4-DECEN-1-AL
Chloral hydrate
3-Chloro-2-hydroxyphenylboronic acid
Methyl 1H-imidazole-4-carboxylate
3-Chloro-2-methylpyridine
Isoquinoline-8-boronic acid
Benzyl 2-isopropyl-4-oxotetrahydro-1(2H)-pyridinecarboxylate