HFF细胞:人包皮成纤维细胞系全国包邮 货号:GD-C7818567
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收后的操作流程与注意事项:1)如果细胞为贴壁细胞,而收到时呈悬浮或者部分悬浮的状态,请将悬浮的细胞即时离心,加15%血清的完全培养基到新的培养皿/瓶继续培养3天;同时原培养瓶中剩下的贴壁细胞更换为15%血清的完全培养基培养3天。3天后若原瓶或者新瓶中的细胞都没有出现增值而是继续脱落死亡,请及时联系实验室技术人员会跟进解决;2)贴壁细胞生长缓慢;适当提高血清浓度(不能超过20%),或可根据该细胞生长密度,考虑胰酶消化后,转移到新的培养瓶继续培养;3)生长不均:贴壁细胞若出现分布不均,成岛状生长,可将细胞进行消化,重悬打散细胞,加入新鲜培养基进行培养。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1,2,5-TriMethylpyrrole, 99%
Triethyl citrate
ALIZARIN GREEN
3-CYCLOPENTYL-1-PROPANOL
1-(1-BroMovinyl)-4-chlorobenzene
1-BROMO-4-DODECYLBENZENE
2-(4-FLUOROPHENYL)OXIRANE
1,2-Diiodotetrafluorobenzene 99%
N-Benzylquinidinium Chloride [Chiral Phase-Transfer Catalyst]
4-(N,N-Dimethylamino)-3-methylphenylboronic acid
(S)-(+)-(3,5-Dioxa-4-phospha-cyclohepta[2,1-a;3,4-a']dinaphthalen-4-yl)morpholine, min. 97% (S)-MorfPhos
CESIUM CHROMATE
3,5-Dibromo-4-methylpyridine
FMOC-L-3-Trifluoromethylphe
6-Nitroindole
1-[4-(BroMoMethyl)phenyl]-1H-pyrrole
4,6-Dinitroresorcinol (wetted with ca. 20% Water, containing 5g on a dry weight basis)
(1-(4-BroMophenyl)cyclopropyl)Methanol
Silver(I) fluoride, 99+%
Mineral Oil, White, MediuM, FCC
2-NitrocinnaMic Acid
3-fluorobenzotrichloride
Methyl 3-chloro-4-hydroxybenzoate
2-Methylbenzimidazole
4-[(4-aminophenyl)azo]phenol
(+)-DIBENZOYL-L-TARTARIC ANHYDRIDE
DIMETHYL-D6 SULFATE
N-BroMosucciniMide
Ethyl pyrazolo[1,5-a]pyriMidine-3-carboxylate, 95%
1,1,1-Trifluoro-2,4-pentanedione, 98%
N-Cyclohexyl-3-nitrobenzenesulfonaMide, 97%
MethylcobalaMin
5-Fluoro-2-Methoxypyridine-3-boronic acid pinacol ester
TIN PHOSPHIDE
(3S)-2-CARBOBENZOXY-1,2,3,4-TETRAHYDROISOQUINOLINE-3-CARBOXYLIC ACID
2-Fluoro-4-Methylaniline
SPADNS
3-Fluorobenzyl Chloride
Triaza-12-crown-4, 99%
2,2-Pentamethylene-1,3-dioxolan-4-one
2-Formyl-2-phenylacetonitrile
5-CHLORO-2-METHOXYPHENYLTHIOUREA
SODIUM TELLURATE
4-(1,1,2,2-Tetrafluoroethoxy)toluene
2-Diphenylphosphinobenzaldehyde
Methyl 2-[1-Methyl-3-(trifluoroMethyl)-1H-pyrazol-5-yl]benzoate
4'-(DiMethylaMino)benzylidene-4-nitroaniline
3-(3-BroMophenyl)-5-ethyl-1,2,4-oxadiazole
SodiuM biphenyl, 20%w/w solution in diethylene glycol diethyl ether, offered as 20 x 15ML
LithiuM Hydroxide, Monohydrate, GR ACS
2-Methyl-1-pentanol
2-Bromo-5-trifluoromethylphenol
2-chloro-3-nitrotoluene
2-amino-4-chlorobenzenethiol
Azobenzene
3-(3-HYDROXYPHENYL)PROPIONIC ACID
5,5,5-TRIFLUORO-DL-LEUCINE
2'-AMINO-4',5'-DIMETHOXYACETOPHENONE
1-BOC-6-chloroindole-3-boronic acid, pinacol ester
3,4-Dihydro-2H-benzo[1,4]thiazine
N-(5-Anilino-2,4-pentadienylidene)aniline hydrochloride, 98%
Bombesin
Decanamide
3-Hydroxypiperidine hydrochloride
4-Fluorobenzylamine
2-Methyltetrahydrofuran
5-AMINO-2,3-DICYANO-1,4-NAPHTHOQUINONE
3-(4-Methyl-4H-1,2,4-triazol-3-yl)aniline
(S)-(-)-3-Benzyloxy-1,2-propanediol
5-Chloro-2-(MethoxyMethoxy)phenylboronic acid
Biuret, 97%, extra pure
Ethyl 3-ethoxybut-2-enoate, 97%
n-Octanal
Methyl 3,5-DibroMo-4-hydroxybenzoate Monohydrate
Dimethyltin dichloride
D-Tyrosine,N-[(1,1-dimethylethoxy)carbonyl]-3-iodo-
dihydro-fk 506
Cephalomannine
FPSA ELISA Kit
CD30L ELISA Kit
Human Netrin-4,Ntn4 ELISA Kit
Human I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit
cGMP ELISA Kit
Rat procollagen Ⅲ N-terminal peptide,PⅢNT ELISA Kit
Ntn1 ELISA Kit
Human C1INH-IgG ELISA Kit
Human ATP-binding cassette transporter G2,ABCG2 ELISA Kit
PGE2 ELISA Kit
Autoinduction Terrific Broth Medium
Selective Cattle Heart Broth Enrichment Medium
AntiMony(III) n-butoxide, 99+%
2-FLUORO-5-IODOBENZOTRIFLUORIDE
5-METHOXY-2-METHYL-3-INDOLEACETIC ACID
2-METHOXYTHIOANISOLE
3-fluorobenzotrichloride
(2-bromo-2-nitrovinyl)benzene
dimethyl ethylphosphonate
4-butylaniline
DIPHENYLTIN DICHLORIDE
4-METHOXY-3-NITROBENZALDEHYDE
4-Fluoro-3-formylphenylboronic acid
4,4'-Oxybis(benzoic acid), 98+%
1,4-Dioxaspiro[4,5]dec-7-en-8-boronic acid pinacol ester, 96%
Myristoyl chloride
4-(4-Methyl-1-piperazinyl)benzeneboronic acid pinacol ester
7-PENTADECANONE
2-METHYL-4-PHENYL-2-BUTANOL
Zaltoprofen
2-METHOXY-4-NITROPHENYL ISOTHIOCYANATE
1-Octanesulfonic acid, sodium salt, anhydrous, Ion pair chromatography, 99+%
Anisolemethoxy benzene
(2S,5S)-2,5-Diphenylpyrrolidine
3-(3-Chloropropylcarbamoyl)benzeneboronic acid 98%