HCC827细胞：人肺癌细胞系更多培养技术支持 货号：GD-C7718937
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结：1)培养基中血清的比例多少合适？血清是细胞培养上一个极为重要的营养来源，所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%，不要低于5%或高过20%，因为含量过低会导致细胞营养不足，过高则会破坏渗透压平衡。一般建议血清含量为10%，可以适量加减；2)何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上的更换时间，按时更换培养基即可。还可以参照指示剂的颜色变化进行更换；3)购买的复苏细胞，为何会有脱落？因为运输的过程中不可避免的会有震荡，收到后可以离心收集；4)购买的细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？研究人员在冷冻细胞之培养时出现细胞数目太少，大都是因为离心过程操作上的失误，造成细胞的物理性损伤，以及细胞流失。建议细胞解冻后不要立刻离心，应待细胞生长隔夜后再更换培养基即可；5)购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久；6)收到的冷冻管瓶身破裂，瓶盖有裂纹，或瓶盖脱落之原因？冷冻管瓶盖裂纹，或瓶身破裂，可能是因为操作者夹取冷冻管时用力不当，造成冷冻管裂损，建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱，乃因热胀冷缩之物理现象，冷冻管有可能因此而造成细胞污染，故冷冻管于放入和取出液氮桶时，均应立刻将冷冻管再一次扭紧。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-tert-ButyldiMethylsiloxy-3-Methoxybenzeneboronic acid, 98%
2-AcetaMido-3,4,6-tri-O-acetyl-2-deoxy-α-D-glucopyranosyl chloride
4-BroMothiophene-3-carbaldehyde
N-[(9H-Fluoren-9-ylMethoxy)carbonyl]-4-nitro-L-phenylalanine , 98%
4-Methylphenyl-L-alanine
5-Hydroxy-1,4-naphthalenedione
BIS(TRICHLOROSILYL)ACETYLENE
(2-Chlorophenyl)glycine
Potassium heptafluoroniobate
Methyl 3-bromobenzoate, 98%
Gallium(III) acetylacetonate (99.99+%-Ga) PURATREM
1-Boc-pyrazole-4-boronic acid pinacol ester
3-Methylcrotonoyl chloride
DIISOAMYLAMINE
1-(Mesitylene-2-sulfonyl)-3-nitro-1,2,4-triazole
2-(2-AMinoethylaMino)ethanol
4,4'-DibroMo-trans-stilbene
2,4-DibroMo-5-Methylanisole
Toluidine Blue O, pure
Phenacetin, Powder
TetraMethylthiourea
dipotassium malate
Vinyltri-t-butoxysilane
2-benzoyl-1,2-dihydroisoquinoline-1-carbonitrile
Sodium succinate
1-NAPHTHYL PROPIONATE
BIS(T-BUTYLCYCLOPENTADIENYL)TITANIUM DICHLORIDE
3,5-Dichloro-2,4,6-trifluoropyridine
Di-n-pentyl phthalate, 98%
1-Boc-3-Methoxyazetidine, 95%
Benzyl acetate
TriethylenetetraMine tetrahydrochloride
Phosphotungstic acid hydrate
RhodiuM(III) oxide pentahydrate
4-Nitrophenyl isocyanate
1-BOC-6-benzyloxyindole
4-Cyano-1H-imidazole-5-carboxamide, 97%
o-Acetoacetanisidide
Tin(II) hexafluoroacetylacetonate (99.9%-Sn)
1-(4'-Bromophenoxy)-1-ethoxyethane, tech., 90%
2,4-Difluoro-3,5-dichloronitrobenzene
2,15-HEXADECANEDIONE
D-Leucinol
(-)-Diisopinocampheyl chloroborane
4-Nitrobenzoic acid
3,5-Bis(trifluoroMethyl)benzaMidine hydrochloride
2-[2-(Furan-2-yl)vinyl]-4,6-bis(trichloroMethyl)-1,3,5-triazine
3-AMino-6-chloropyrazine-2-carbonitrile
2-[(5-Nitro-2-pyridyl)oxy]ethan-1-ol, 97%
3-INDOXYL PHOSPHATE DISODIUM SALT
DiMethyl 1H-IMidazole-4,5-dicarboxylate
Magnesium Hydroxide
Acid Red 315
4-(Hydroxyphenyl)-1-heptanone
2,4-difluorocinnamic acid
3,3-Tetramethyleneglutarimide
(S)-1-(TERT-BUTOXYCARBONYL)-2-AZETIDINEMETHANOL
N-(Furan-2-ylMethyl) 4-(4-broMopyrazol-1-yl)benzenesulfonaMide
Enoxolone
Cyclopamine
1,4-Butanesultone
3-Iodo-1H-pyrazole
7-Methylthieno[3,2-d]pyrimidin-4-hydrazine
Metformin
beta-Sitosterol
N-HEXYLTRIETHOXYSILANE
Iron powder reduced
cis,cis,cis,cis-1,2,3,4-Cyclopentanetetracarboxylic acid
1,3-DibroMo-2-propanol
2,2'-Methylenebis(6-broMo-4-chlorophenol)
1,2-Benzisoxazol-6-ol
1,3-DibroMo-2,2-diMethoxypropane, 99%
C8 CERAMIDE
Hexaphenyldisilane
4-BROMO-2,3,5,6-TETRAFLUOROBENZOTRIFLUORIDE
2-Bromo-4-fluorobenzonitrile
DL-1-Phenylethanol
Andrographolide
ANP ELISA Kit
β-EPR ELISA Kit
PC4 ELISA Kit
Human CLDN9 ELISA Kit
Chicken Immune RNA,Irna ELISA Kit
CKM ELISA Kit
Chicken 5-Nucleotidase,5-NT ELISA Kit
Human SULT1E1 ELISA Kit
Human antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA Kit
IAP ELISA Kit
Oatmeal Agar
Enterotoxin toxin-producing medium without Agar
Isoniazid
SodiuM zirconiuM oxide, 98%
5-AMino-2-iodopyridine, 95%
2-Sulfobenzoic acid hydrate, 98%
dipotassium malate
Cobaltous nitrate
trisodium phosphate anhydrous
2-oxa-6-azaspiro[3.3]heptane
GALLIUM NITRIDE
PHENOLDISULFONIC ACID
Dibenzyl ether, 98+%
3-Methylbenzo[b]furan-2-carboxylic acid, 97%
N-Boc-D-^b-proline, 95%
VinylMagnesiuM chloride solution
CARBONYLBROMOBIS(TRIPHENYLPHOSPHINE)RHODIUM(I)
6-BroMo-5-Methylpyridine-3-boronic acid
(S)-Boc-3-(2-naphthyl)-β-Ala-OH
6-HYDROXY-2-NAPHTHYL DISULFIDE
CIBACRON BRILLIANT YELLOW 3G-P
3-Fluoro-4-(propylcarbamoyl)benzeneboronic acid 98%
2',4'-Dichlorobutyrophenone
Trihexylamine
3',4',5-Trichlorosalicylanilide
