PKN-012海马神经元细胞系液体培养基 货号：GD-CP139718
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞，说一点自己培养细胞方面的看法：1)培养细胞前，可以看看细胞特点说明书，包括细胞正常生长的状态图、传代、培养基的类型等；2)不同细胞生长周期不同，有的生长很快，比如说MDA-MB-231，传代的时候取离心悬液的十分之一就已经足够了，有的又是特别慢，等的人心焦，BT474就是，传代是一分二，复苏起始的时候两周多才能长满，所以就要在培养细胞的过程中多多摸索了；3)对于娇弱的细胞，就得细心呵护了，胰酶消化不能过久，吹得时候要轻柔，离心时候也要注意转速和时间，每天都要去看一下细胞，肉眼观察培养基状况、显微镜下观察细胞生长状况；4)冻存细胞复苏后第二天Z好更换一次培养基；5)培养箱隔一段时间彻底用酒精棉擦洗一次；6)养细胞的教训：不能偷懒!细胞虐我千百遍，我待细胞如初恋。1)不管买的细胞、惠赠的细胞，刚拿到手赶紧做两件事：检测是否有支原体污染; 迅速扩增冻存；2)发现有污染迅速处理掉，特别是当你养了很多种细胞时; 细菌污染，真菌污染很容易发现，支原体污染的话，细胞会长的慢，买个支原体检测的kit定期检测一下；3)传代细胞日常的值日清洁是必须的，此外还要定期熏蒸；4)细胞的传代一定要规律，保持相同的confluency和传代时间间隔，不要随心所欲或者拖延；5)注意个人卫生。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
IMazalil
Butyl salicylate
2,5-Difluorobenzophenone, 97+%
IRON SILICIDE
4-Chlorophenylacetylene
Isopropyl Benzoate , 99%
Indole-3-propanamide ,98%
Adipate
2-(Dimethylvinylsilyl)pyridine
Benzyl 2-aminoacetate hydrochloride
Food Red No. 3 Aluminum Lake
Octyl 4-methoxycinnamate
2-Piperidinoethanol
2-Aminobenzenesulfonamide
4-Propylbenzoic acid
3-Methylglutaric anhydride
[[(3,5-DiMethyl-1H-pyrrol-2-yl)(3,5-diMethyl-2H-pyrrol-2-ylidene)Methyl]Methane](difluoroborane)
2,4-Difluoro-3-hydroxybenzaldehyde
PotassiuM hexafluoroantiMonate, 98%
1,2-DIETHYLBENZENE
4,4'-Diiodobiphenyl
N-acetyl-L-aspartic acid
4-Chloro-4'-hydroxybutyrophenone
4-nitrobenzenediazonium hexafluorophosphate
1,7-Dihydroxynaphthalene
2-BROMO-6-CHLORO-4-FLUOROANILINE
Pentafluoropyridine
3,5-Difluorobenzoic acid
(S,S)-N-(p-Toluenesulfonyl)-1,2-diphenylethanediaMine(chloro)(p-cyMene)rutheniuM(II)
Phenyl carbaMate, 98+%
Ethyl propionylacetate
AluMinuM-nickel catalyst
Iodine trichloride
N-Ethyl 2-broMo-5-fluorobenzaMide
3-Fluorobenzoyl chloride
Fennel Oil , 80%
trans-4-Methyl-cyclohexylamine hydrochloride
1H,1H,7H-Dodecafluoro-1-heptanol
Lithium t-butoxide, 98+%
(+)-Griseofulvin, 97%
Methyl 3-amino-2-pyrazinecarboxylate
DL-Thioctic acid
cis-2,6-Dimethylmorpholine
4-Hydroxyisophthalic acid
Citronellal
SodiuM tetraborate
6-TrifluoroMethyl-4-pyriMidinol
2-AMino-4-chlorobenzoic acid, 98%
TriMethylhydroquinone, 97%
DIETHYLCARBAMAZINE CITRATE
N-Methylsaccharin
4-Pyridinecarbonitrile,3-nitro-
2-fluoro-4-(trifluoromethyl)benzaldehyde
1H-indazol-4-amine
2-bromo-5-nitro-1,3,4-thiadiazole
(S)-(-)-Tetrahydro-2-furoic acid
METHYL 2-ACETAMIDOBENZOATE
5-Fluoroindole-2-carboxylic acid
YTTRIUM ALUMINUM OXIDE
4-Hydrazinobenzene-1-sulfonaMide hydrochloride
Dodecanedioyl dichloride, 98%
Ethyl 4-(4,4-dimethylpiperidino)benzenecarboxylate
9,10-Dimethylanthracene, 97%
Aceanthrenequinone, 95%
4-Hydroxy-2-methoxybenzaldehyde
Undecanoyl chloride
1-(4-AMINOPHENYL)-4-(4-METHOXYPHENYL)
2-CHLORO-5-METHYLBENZOIC ACID
1-Pentanesulfonic acid
S-(N,N-DiMethylthiocarbaMoyl)thioglycolic Acid
3-BroMopyridazine
SodiuM hydride, 60% dispersion in Mineral oil, in soluble bags
Mepivacaine Hydrochloride, USP
Glycyl-DL-valine
Propiolic acid
4-Pyrimidinamine,6-chloro-5-nitro-
hept-1-yne
N,N,N',N'-Tetramethylethylenediamine
HSP-90 ELISA Kit
Flt-3l ELISA Kit
Human hnRNP H ELISA Kit
DRD3 ELISA Kit
Monkey TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
Rat IL-12/p40 (Interleukin 12 p40) ELISA Kit
TRAIL-R3 ELISA Kit
Human Proteinase 3 Antibody,PR3Ab ELISA Kit
PDGF.BB ELISA Kit
AIRE ELISA Kit
Skirrow Agar Base
Skirrow Agar Base
MagnesiuM acetate tetrahydrate
trans-Chalcone, 97%
4-n-ButylbenzenesulfonaMide, 97+%
2-Phenoxyethyl broMide, 98%
N-acetyl-L-aspartic acid
4-Methylsulfonylphenol
(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methanol
2,6-Dihydroxynaphthalene
Furfural
(3-((6-Chloro-3-pyridinyl)methyl)-2-thiazolidinylidene)cyanamide
Phosphorus OxybroMide
PotassiuM orthovanadate, 99.9% (Metals basis)
GadoliniuM galliuM isopropoxide, 99.9% (Metals basis), 25% w/v in isopropanol
N-Boc-trans-4-fluoro-L-proline Methyl ester
N,N′-Diethyl-1,3-propanediaMine
Di-tert-butylphosphine oxide
GMBS
Methyl 5-broMo-2-chlorobenzoate
cis-3-Hexenyl 2-Methylbutanoate
2-Fluorobenzylamine ,97%
Boc-D-3,3-diphenylalanine
4-Bromo-2-chloropyridine-5-boronic acid
Methyl 5-chloro-2,6-dihydroxy-4-pyrimidinecarboxylate
