MKN-74细胞:人胃癌细胞系(开学季大促销) 货号:GD-C7520543
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 1H-indazole-4-carboxylate, 95%
Oxadiazon
2-BROMOETHYL ISOTHIOCYANATE
Methyl 2,3-Di-O-benzoyl-4,6-O-benzylidene-alpha-D-glucopyranoside , 98%
TELLURIUM TETRABROMIDE
1-BOC-4-(4-nitrophenyl)
Pentacene ,98%
3-[4-(Trifluoromethoxy)phenyl]propanoic acid
6-(Dimethylamino)pyridin-3-ylboronic acid
Neurodye RH-414, pure
3,3-Dimethylpentane, 99%
TRI-N-BUTYLTIN METHOXIDE
4-Propylresorcinol
(S)--2-carboxylic acid dihydrochloride
2-ChloroMalonaldehyde
2-(1-Methoxy)propyl acetate
3,3'-Dihexyl-2,2'-bithiophene
2-(Pyrrolidine-1-carbonyl)-4-fluoro-1-broMobenzene
3-Fluorobenzaldehyde, 98+%
Triolein
Isobutyltrichlorosilane
ethyllithium
Cyclopropyl Acetylene
Xanthylium, 3,6-bis(diethylamino)-9-[2-(ethoxycarbonyl)phenyl]-, molybdatetungstatephosphate
butyl cinnamate
2-IODO-3-HYDROXYPYRIDINE
3-METHANESULFONYLAMINOPHENYLBORONIC ACID, PINACOL ESTER
1,3-Thiazol-2-YlMethanol
2-(TrifluoroMethoxy)benzyl broMide, 97%
ForMaldoxiMe triMer hydrochloride, 95%
3-AMinophenylboronic acid hydrochloride
4-Chloro-3-Methylphenol Standard
6,8-Dichloro-2-(trifluoroMethyl)quinolin-4-ol
N-Methyl 4-broMobenzaMide
AMMoniuM perfluorooctanesulfonate
SodiuM 4-aMinosalicylate dihydrate
2-Nitrobenzenesulphonyl chloride 97%
N-(5-Iodo-2-pyridinyl)acetamide
1-Benzyl-3-hydroxy-1H-indazole, 97%
5-chloro-2-furoic acid(SALTDATA: FREE)
1-(CHLORO-1-PYRROLIDINYLMETHYLENE)PYRROLIDINIUM TETRAFLUOROBORATE
4-ACETOXY-3,5-DIMETHOXYBENZALDEHYDE
2-Bromo-5-nitro-4-picoline
Escitalopram oxalate
4-BroMostyrene
TriphenylantiMony(III)
2,3,5-Trifluorobenzoic Acid
2-Hydroxybenzyl alcohol, 97%
(R)-(+)-N,N-DiMethyl-1-phenethylaMine, 97%
N-Acetyl-L-valine
2-Chloronitrobenzene
Tetrakis(2-Octyldodecyloxy)Silane
2-Chloroisonicotinaldehyde
3-methoxy-2-nitrobenzaldehyde
3-chloro-6,11-dihydro-6-methyldibenzo[c,f][1,2]thiazepin-11-ol 5,5-dioxide
2,4-Difluorophenol
ISOQUINOLINE-3-CARBOXYLIC ACID HYDRATE
2,6-DIMETHYLBENZOQUINONE
5-Fluoro-3-Methylsalicylaldehyde
Dibenzo[b,d]thiophen-2-ylboronic acid , May contain varying amounts of anhydride, 97%
Neodymium(III) i-propoxide (99.9%-Nd) (REO)
2-Chloro-6-fluorobenzeneboronic acid 97%
n-Pentylmethyldichlorosilane
Fluvastatin sodium salt
H-D-ARG(NO2)-OME HCL
3-Chloro-4-methylphenyl isocyanate
3,5-Dibromosalicylaldehyde
4-Pentynoic acid
1-Hexadecene
SodiuM DL-3-Hydroxybutyrate
2-(N,N-BisBOC-AMino)-5-broMopyriMidine
1-(3-BroMobenzyl)-1H-iMidazole, 97%
METANIL YELLOW
1,1-DiMethyl-3-[3-(trifluoroMethyl)phenyl]urea
(2R,3R)-(-)-dibenzoyl-L-tartaric acid anhydrous
Sodium Molybdate
Sulfotep
Methyl phenyl sulfoxide
NT-2R ELISA Kit
Jo1/HRS ELISA Kit
Scl-70 Ab ELISA Kit
Human PYY (Peptide YY) ELISA Kit
Monkey PAF (Platelet Activating Factor) ELISA Kit
Rat γ-GT1 (Gamma Glutamyltransferase 1) ELISA Kit
ANG-Ⅰ ELISA Kit
Human PKCη (Protein Kinase C Eta ) ELISA Kit
Human PA-IgG/M/A ELISA Kit
TP63 ELISA Kit
Lauryl Sulfate Tryptose Broth
Bile Lactose Medium
Ethanesulfonic acid, 97%
Phenothiazine, 98+%
4-n-Pentyloxyphenylacetylene, 99%
2-Iodo-5-(TrifluoroMethyl)Benzyl BroMide
ethyllithium
Aminopyridineoxide; 98%
Acid Orange 89
2,4-Dimethylthiazole
4-METHYLTHIAZOL-2-YLGUANIDINE HYDROCHLORIDE
Arabic gum
Methyl 3-Fluoro-4-Nitrobenzenecarboxylate
N-[3,5-Bis(trifluoroMethyl)phenyl]thiourea, 98+%
1-n-Butyl-3-MethylpyridiniuM tetrafluoroborate, 99%
2-AMino-5-Methylbenzoic acid
4-Methyl-5-vinylthiazole
3-AMino-2,5-dichlorobenzoic acid
4-Fluorophenylboronic acid, neopentyl ester
AztreonaM
3-Methoxy-5-(trifluoroMethyl)aniline
(-)-2,3-O-Isopropylidene-D-threitol
Diacetone-D-glucose ,98%
Benzamidine hydrochloride ,99%
8-Methoxy-3,4-dihydro-1H-naphthalen-2-one