hTERT RPE-1人视网膜色素上皮细胞系液体培养基

hTERT RPE-1人视网膜色素上皮细胞系液体培养基 货号：GD-CP138780

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Fluoro-5-(MethoxycarbonylMethyl)benzeneboronic acid pinacol ester, 96%
2-BroMo-5-hydroxypyrazine
2-ChlorobenzaMide
2′,4′-DiMethylacetanilide
4-Nitro-N-MethylphthaliMide
2,2-DIFLUOROCYCLOPROP-1-YLAMINE HYDROCHLORIDE
4-BroMo-N,N-diMethyl-3,5-xylidine
(tetrahydrofuran-2-ylmethoxy)acetic acid(SALTDATA: FREE)
Dabsyl-L-alanine
Methyl propyl disulfide, tech., 80%
Piceid
2-CHLORO-6-METHYLPYRIDINE-4-CARBONYL CHLORIDE
Propantheline bromide
3,3'-Diamino-4,4'-dihydroxydiphenyl sulfone
[Hydroxy(tosyloxy)iodo]benzene
Ethanesulfonyl Chloride
2H,3H-Decafluoropentane
6-BroMo-[1,2,4]triazolo[1,5-a]pyrazine
tert-Butylacetyl chloride, 99%
Methylparaben, NF
1-Isopropyl-4-piperidone
biperiden
prifinium bromide
4-Hydroxy-6-methyl-2-pyrone
dihydrocoumarin
CYCLOHEPTYLACETIC ACID
PHENYLSELENOL
Benzyl Salicylate
DiMethyl 2-Methyladipate, 97%
1,3-DibroMo-5-fluoro-2-iodobenzene, 97%
7-Chloro-1H-pyrrolo[2,3-c]pyridine
DL-Ala-DL-Ala
Methyl 4-iodo-3-nitrobenzoate, 99%
SOLVENT BLUE 38
4-(Morpholine-4-carbonyl)broMobenzene
DIMETHYL 3-NITROPHTHALATE
Ethylmagnesium Bromide (39% in Ethyl Ether, ca. 3mol/L)
2-(Aminomethyl)-1,3-dioxolane
Hexafluoroethane (FC-116) 99.99%
Carboxy-PTIO
HEXAPHENYLDISILOXANE
Calcium pyruvate
DIMETHYL 3-NITROPHTHALATE
Cyanamide
3-AMino-1,2,4-triazole-5-carboxylic Acid HeMihydrate
TetraMethylaMMoniuM fluoride
2-(TrifluoroMethoxy)phenol
2-Ethyl-2-(hydroxyMethyl)-1,3-propanediol, 98%
3-BroMoadaMantane-1-carboxylic acid, 97%
Iridium trichloride
2-Cyclopenten-1-one
Glycine, N-(2-aminobenzoyl)-, phenylmethyl ester
4-Pyridinecarboxamide, N-methoxy-N-methyl-
Pyridine, 4-(1H-imidazol-4-yl)-
S-(4,5-dihydro-2-methyl-3-furyl) ethanethioate
1,3-DIAZASPIRO[4.5]DECANE-2,4-DIONE
TRIISOPROPOXYVANADIUM(V) OXIDE
2-BroMo-3,5-diMethoxytoluene
(1S,2S)-trans-N-Boc-1,2-cyclopentanediaMine
Phthalide
3-chloroquinoxaline-2-carboxylic acid(SALTDATA: FREE)
Disodium 4,4'-Bis(2-sulfonatostyryl)biphenyl
Dimethyldithiocarbamic Acid Allyl Ester
1-PHENYL-3-THIOSEMICARBAZIDE
6-Formyl-2,3-dimethoxybenzoic acid
Rosuvastatin
2-BROMO-N,N-DIETHYLETHYLAMINE HYDROBROMIDE
2-Acetylphenylboronic acid
1,1-Bis(4-hydroxyphenyl)cyclohexane
Ethyl 4-hydroxy-8-Methoxyquinoline-3-carboxylate
AMberlyst? A-21, ion-exchange resin
5-BroMoindoline, 97%
4-BroMo-2,5-difluorobenzenesulfonyl Chloride
DisodiuM 4-Nitrophenyl Phosphate Hexahydrate
4-methoxy-m-phenylenediammonium dichloride
1,3,2-Dioxaborolane, 2-(9,9-dioctyl-9H-fluoren-2-yl)-4,4,5,5-tetramethyl-
Octahydro Cyclopenta (b) Pyrrole-2-Benzyl Carboxylate Hydrochloride (Recemiac)
Piperidine, 1-[(2S)-2-pyrrolidinylmethyl]-
Porcine TXB2 (Thromboxane B2) ELISA Kit
IL17 ELISA Kit
Human CNTROB ELISA Kit
Human CACYBP (Calcyclin Binding Protein) ELISA Kit
PROG ELISA Kit
C-Peptide ELISA Kit
Monkey sTNF-1 (Soluble Tumor Necrosis Factor 1) ELISA Kit
Human intestinal fatty acid binding protein，iFABP ELISA Kit
Human SEMA4C ELISA Kit
AQP2 ELISA Kit
The culture medium of Streptococcus vaccine
Brucella Agar
MagnesiuM Stearate
CalciuM silicate, Meta, Reagent Grade
5-Fluoro-2-Methylbenzyl chloride, 99%
2-Methylpyrazine-5-Carboxylic Acid
biperiden
3,4-Dimethoxyphenylmagnesium bromide
3-Methyl-1H-1,2,4-trizole
5-Chloro-8-hydroxy-7-iodoquinoline

TRIETHYLTIN BROMIDE
4,5-DIMETHYL-THIAZOLE-2-THIOL
^a-CyanocinnaMic acid, 96%
DL-Valine ethyl ester hydrochloride, 99%
3-IndoleMethanol, 97%
2-Chloro-4-fluorotoluene
2-ThiopheneMethanol
DL-2-HYDROXY-3-BUTENOIC ACID METHYL ESTER
2,3-DibroMopropionic acid
5,7-Dichloroquinoline
4-NITRO-2-(TRIFLUOROMETHYL)BENZONITRILE
(R)-?-Homovaline
6-(Trifluoromethyl)uracil
(3S)-3-Hydroxy-4,4,4-trifluorobutanoic acid
2-(4-Aminophenoxy)naphthalene