HHSteC人肝脏星形细胞系液体培养基

HHSteC人肝脏星形细胞系液体培养基 货号：GD-CP139618
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难，Z关键几点：1)所有的东西使用Z好的，如果你用的血清、培养皿、培养基、胰酶什么的都是进口的，真的很难相信你会把细胞养坏；2)动作要快，胰酶消化，换液什么，细胞暴露在空气中的时间越少越好。另外，要掌握好胰酶消化时间，所谓的细胞状态差，很多时候是传代的原因；3)有时间的话，需要细胞计数，传相应数目的细胞到培养皿中，可以大致清楚细胞的生长曲线，不会临时要处理，手足无措；4)要做什么心里要非常清楚，合理安排时间，传代细胞的实验穿插进行，可以节省很多时间，也不会耽误别人的实验；5)个人的实验器具自己收一套，不要和别人混用，Z近换了什么新的东西稍微记一下，试剂一旦怀疑污染，马上丢；6)传代细胞不能进去太多人，避免相互干扰。每天对待细胞比孝敬爹妈还用心，那真是捧在手里怕碎了，含在嘴里怕化了，看在眼里怕丢了，培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作，多喷喷酒精，要是对灭菌的东西不放心，自己包自己送自己烘干；2)不要和别人共用试剂耗材，自己准备一套；3)有可能的话在拿到新细胞的时候做好支原体衣原体检测；4)水浴锅很脏，生化培养箱要是得手动加湿的话盘里的水也会很脏，勤换(灭菌水加进去)；5)晚上离开的时候Z好给传代细胞照紫外，超净台是用完就开；6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
FMoc-3-(3-Pyridyl)-D-Alanine.HCI
Acridone
2-METHOXY-5-(TRIFLUOROMETHYL)BENZALDEHYDE
1-(2-Fluorophenyl)biguanide hydrochloride
RHODAMINE 6G PERCHLORATE
4-Methoxybenzenesulfonyl chloride
L-(-)-Tyrosine
Thioacetamide
2-[4(1H)-Pyridinylidene]indan-1,3-dione
4-(3-Hydroxypropyl)benzeneboronic acid 96%
Triphosphopyridine nucleotide disodium salt
Isoflavone
Z-GLN(TRT)-OH
5-AMINO-2,4-DIFLUOROBENZOTRIFLUORIDE
Butyl chloroacetate
2-AMino-5-Mercapto-1,3,4-thiadiazole
exo-3,6-Epoxy-1,2,3,6-tetrahydrophthalic Acid
4-Carboxyphenylacetic acid
Ethyl cis-2-aMino-1-cyclohexanecarboxylate hydrochloride, 95%
BIFENTHRIN
2-Chloro-3,4-diMethoxybenzaldehyde
2-Pyridinecarboxaldehyde
(S)-(+)-2-Phenylpropionic acid
4-Methyl-2-phenylthiazole
1,3-dinitronaphthalene
1,4-BIS(TRIMETHYLSILYL)-1,3-BUTADIYNE
Benzofuran-2-boronic acid
Cobalt(II) Oxalate Dihydrate
Copper(I) telluride, 99.5% (Metals basis)
7,8-Dihydroxyflavone hydrate, 97%
4-tert-ButyldiMethylsiloxy-3-Methoxybenzeneboronic acid, 98%
2-AcetaMido-3,4,6-tri-O-acetyl-2-deoxy-α-D-glucopyranosyl chloride
4-BroMothiophene-3-carbaldehyde
N-[(9H-Fluoren-9-ylMethoxy)carbonyl]-4-nitro-L-phenylalanine , 98%
4-Methylphenyl-L-alanine
5-Hydroxy-1,4-naphthalenedione
BIS(TRICHLOROSILYL)ACETYLENE
(2-Chlorophenyl)glycine
Potassium heptafluoroniobate
Methyl 3-bromobenzoate, 98%
Gallium(III) acetylacetonate (99.99+%-Ga) PURATREM
1-Boc-pyrazole-4-boronic acid pinacol ester
3-Methylcrotonoyl chloride
DIISOAMYLAMINE
1-(Mesitylene-2-sulfonyl)-3-nitro-1,2,4-triazole
2-(2-AMinoethylaMino)ethanol
4,4'-DibroMo-trans-stilbene
2,4-DibroMo-5-Methylanisole
Toluidine Blue O, pure
Phenacetin, Powder
TetraMethylthiourea
dipotassium malate
Vinyltri-t-butoxysilane
2-benzoyl-1,2-dihydroisoquinoline-1-carbonitrile
Sodium succinate
2-Chlorofluorobenzene
ISOTHIOCYANIC ACID 4-PENTEN-1-YL ESTER
(E)-3-(diMethylaMino)-1-phenylprop-2-en-1-one
AMberlite IRA-68, ion exchange resin
2-(Aminomethyl)-4-methylthiophene 97%
2'-Deoxy-5-hydroxymethyluridine ,98%
4-(2-Pyridinyl)-1H-pyrazol-5-amine
Tetra-n-octylphosphonium Bromide
3-Chloro-4-fluorobenzonitrile
6-AZATHYMINE
Ethyl chlorothioformate
1-Naphthol;1-Naphthalenol;α-Hydroxynaphthalene;1-Hydroxynaphthalene
LanthanuM iodide
2,2,2-Trifluoroethyl TrifluoroMethanesulfonate
Etoposide
N-Acetyl 3,5-dibroMoaniline
(R)-N-BOC-Propargylglycine, 95%, 98% ee
ESERINE
6-Azauridine
Hexamethyldisilane
urease from jack beans
Cyclohexanecarboxaldehyde
Hexatriacontane
CTAGE ELISA Kit
CD147 ELISA Kit
GLP-2 ELISA Kit
Human APLP1 ELISA Kit
NK ELISA Kit
Il-34 ELISA Kit
IL12A ELISA Kit
Human BIRC4/API3 ELISA Kit
Human NYX ELISA Kit
RAD6 ELISA Kit
BCT Enrichment Broth
Nodule Bacteria Medium YM
Ethylenzene
trans-5-Decene, 97%
PotassiuM 4-acetylphenyltrifluoroborate, 97%
2-AMinonaphthalene-1-sulfonic acid, 98%
2-Pyridinecarboxaldehyde
Bendamustine
Ginsenoside Rg3
4-aminobenzoic acid methyl ester

4-NITROPHENOXYACETONITRILE
4-AMino-2-BroMobenzonitrile
DiMethoxydiMethylsilane, 97%
3-Chloroanisole, 98+%
Ethyl 3-hydroxybenzoate, 99%
2-Iodotoluene
3-BROMO-4-METHYLPHENOL
2-Chloro-6-(trifluoroMethyl)pyridine
(-)-1,2-BIS((2S,5S)-2,5-DIMETHYLPHOSPHOLANO)ETHANE
4-PENTYLANILINE
3-(2-HydroxyethylcarbaMoyl)phenylboronic acid
4-Bromoresorcinol
2-methylquinolin-5-amine(SALTDATA: FREE)
1-(4-Methoxyphenyl)-2-thiourea 98%
Isobutyl 4-Hydroxybenzoate