RKO-AS45-1人结肠癌转基因细胞系液体培养基

RKO-AS45-1人结肠癌转基因细胞系液体培养基 货号：GD-CP139267

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-AMino-4-nitrophenol, 98%
3,5-DiMethylisoxazole-4-boronic acid pinacol ester
3-BroMoquinoline
2-BroMo-4-chloro-6-fluorophenol, 97%
4-(Methylthio)biphenyl-3-carboxylic acid
(2-CARBAMOYLPHENOXY)ACETIC ACID
(TERT-BUTOXYCARBONYLMETHYL)TRIPHENYLPHOSPHONIUM CHLORIDE
4-(Dimethylamino)pyridine N-Oxide Hydrate
gamma-Methyl-gamma-decanolactone
DL-beta-Amino adipic acid
Triethoxysilylpropylethylcarbamate
3-Amino-2-chlor-6-methylphenol
3-(1-ACETYL-,4-DIHYDROPYRID-4-YL)INDOLE
4-N-OCTYLOXYBROMOBENZENE
Methanol
2-Methyl-3-nitroaniline
1,2-Distearoyl-sn-glycero-3-phosphocholine
Methyl 2-broMo-4-Methoxybenzoate
2,6-Dichloro-4-fluorophenol, 99%
SulfaMethazine, USP
Tetraisobutylene (contains isoMer)
3-Hydroxy-4-methoxybenzoic acid
4-Chloroisatin
2,9,10-trimethoxy-5,13a-dihydro-6H-isoquino[3,2-a]isoquinolin-3-ol
5-hydroxy-4-oxo-4H-pyran-2-carboxylic acid
2-ACETYL-1-TETRALONE
Anthranilonitrile
Z-D-HoMoserine Lactone
ThuliuM(III) sulfate octahydrate, 99.99%
2,4,5-TriMethoxybenzoic acid, 99%
Propiconazole
5-Br-PADAP
4-(4-CYANOPHENYL)MORPHOLINE
O-ACETYLSALICYLOYL CHLORIDE
4-Methoxyphenacyl chloride
TRIISOPROPYLPHOSPHINE
Cobalt(II) sulfate hydrate, (trace metal basis), 99.999%
2-Benzimidazolepropionic acid, 97%
N,N-Diethyl-2,3,3,3-tetrafluoropropionamide
n-Octanoic Acid Isopropyl Ester
Fmoc-D-Aspartic acid beta-tert-butyl ester
(3S)-(-)-3-(DIMETHYLAMINO)PYRROLIDINE
2,4,5-Trifluoro-3-methylbenzoic acid
Methyl 2-bromopropionate
1-Iodo-2-Methylpropane (stabilized with Copper chip)
AMygdalin
(R)-2-Hydroxy-4-phenylbutyric Acid
3-(ChloroMethyl)-2-fluoropyridine
6-Thien-2-ylnicotinic acid, 97%
4-[(tert-Butoxycarbonylamino)methyl]benzoic acid
ChloroMethyl Chlorosulfonate
L-.alpha.-Aminosuberic acid
2-chloro-4-methoxyphenol
4,6-dihydroxy-5-methoxypyrimidine
Sudan Orange G
beta-Bromostyrene
1-(3-Chloro-2-Methylphenyl)pyrrolidine
2,3,5-TRIFLUOROBENZONITRILE
PotassiuM n-pentyltrifluoroborate
2-Fluoro-4-nitroanisole 98%
1-(cyclobutylcarbonyl)(SALTDATA: FREE)
1-Hydroxy-2,2,5,5-tetramethyl-4-phenyl-3-imidazoline-3-oxide
Bis(2-aminophenyl) Sulfide
2-AMINO-4-METHYLOXAZOLE
(2-CARBAMOYLPHENOXY)ACETIC ACID
METHYL NONANOATE
Silicon carbide
Chloroacetyl isocyanate
1-BroMopyrene
N-(2-Methoxyethyl)MethylaMine
N-Cyclopropyl 4-AMinobenzaMide
2-BroMopyridine-4-boronic acid, 95%
Ethyl 4-chloro-2-methylthio-5-pyrimidinecarboxylate
SodiuM 1-Naphthol-4-sulfonate
Chromous acid
1-Bis(4-fluorophenyl)methyl
Calcium hypophosphite
2,6-di-tert-butyl-4-ethylphenol
AQP-1 ELISA Kit
Mouse RasGAP (Ras GTPase Activating Protein) ELISA Kit
Human PCDHα10 ELISA Kit
Human DPEP2 ELISA Kit
CD8 ELISA Kit
Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
GM-CSF ELISA Kit
Human ELA2B ELISA Kit
SAP ELISA Kit
INB-βA ELISA Kit
Dextrose Agar
Sucrose Tryptone Broth
Zinc 2,4-pentanedionate Monohydrate
Aluminum fluoride
5,5-Diphenylhydantoin
2-Fluoro-3-Methylpyridine-5-boronic acid, 97%
3-Hydroxy-4-methoxybenzoic acid
2,6-dimethyl-4-hydroxy pyridine
Tunicamycin
1,3-Pentadiene

Glycine hydrochloride
HEMOCYANIN, KEYHOLE LIMPET
Dibenzyl Disulfide
4-Fluoro-2-Methoxybenzonitrile, 97%
3-AcetaMidophenol, 99%
4-(TrifluoroMethylthio)benzaldehyde
Lovastatin
4-(N-Ethyl-N-(4-Methoxybenzyl)sulfaMoyl)phenylboronic acid
Methyl 3-aMinopropionate hydrochloride
Buchu leaf oil, Crenulata
DocosanaMide
1-Acetyl-4-piperidone
S-Methyl thiazole-4-carbothioate 97%
Calcium bis{4-[(2,4-dihydroxy-3,3-dimethylbutanoyl)amino]butanoate}
2-Bromo-5-(trifluoromethoxy)phenol