RKO-E6人结肠癌转基因细胞系液体培养基 货号：GD-CP139266
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-Nitro-o-phenylenediaMine, 97%
N6-Methyl-2'-deoxyadenosine
TETRAAMMINEPALLADIUM(II) BROMIDE
METHYL LINOLENATE
2-(4-CHLOROPHENYLTHIO)BENZALDEHYDE
(R)-C3-TunePhos
Isophorone diisocyanate
5-Bromobenzo[b]thiophene-3-carbaldehyde , 97%
1-Boc-4-benzyl-
Methyl 2-bromo-4-nitrobenzoate
Ammonium thiosulfate, extra pure, 98+%
2-Methoxypyridine
5-Amino-2-methylpyridine
5'-O-Dimethoxytrityl-deoxythymidine
3-BroMo-2-furoic acid
1,2,5-Oxadiazole-3-carboxylic acid
1,2-DiMyristoyl-sn-glycero-3-phosphocholine
N-Methyl 4-broMo-3-MethoxybenzaMide
(4-Methyl-2-pyrid-4-yl-1,3-thiazol-5-yl)Methanol, 97%
Sulfadoxine, USP
Benzotrifluoride
altretamine
N-Benzylformamide
Dodecyl gallate
4-Hydroxy-3-methylbenzoic acid
3-Methoxycarbonylphenylboronic acid pinacol ester
4-AMINOAZOBENZENE
6-BroMo-2-naphthol
2-Methoxy-5-(trifluoroMethyl)benzaldehyde, 98%
2-(2-Pyrrolidinyl)thiazole, 97%
RubidiuM chroMate
Rubrene
2,3-Dihydro-benzo[1,4]dioxine-6-sulfonyl chloride
O-ALLYLHYDROXYLAMINE HYDROCHLORIDE
2-AMinoethyldiisopropylaMine
2-Chlorophenethyl broMide
1-Butylpyridinium bromide ,99%
4-(ethoxycarbonyl)-3,5-dimethyl-1H-pyrrole-2-carboxylic acid(SALTDATA: FREE)
(S)-4-Benzyl-oxazolidine-2,5-dione
3-Hydroxy-1,2,3,6-tetrahydropyridine, N-BOC protected
HEXAAMMINERUTHENIUM(III) CHLORIDE
3-(TRIFLUOROMETHYL)BENZAMIDE
4-Phenoxypyridine
POTASSIUM HEXAHYDROXOANTIMONATE
Methyl cyclobutanecarboxylate
2-BroMo-5-fluoro-3-Methylpyridine
Benzyl Methyl Malonate
Methyl (2E)-3-(4-Methoxyphenyl)prop-2-enoate
DL-Alanine-beta-naphthylaMide hydrochloride, 98%
Methyl 3-(4-hydroxyphenyl)propionate
Undecafluorohexanoic Acid (ca. 5MMol) [Ion-Pair Reagent for LC-MS]
Ferrous ammonium sulfate
Isononyl alcohol
Antimony tribromide
L-Aspartic acid 1-tert-butylester
2-Hydroxy-6-methylpyridine-3-carboxylic acid
2-Phenoxyethyl chloride
5-BROMO-2-METHOXYBENZENESULFONYL CHLORIDE
(1R,2R,3S,5R)-(-)-2,3-Pinanediol
N-Prop-2-ynylurea , 97%
(2E)-3-(4-Ethoxy-3-methoxyphenyl)acrylic acid
5-Chloro-1H-indole-2-carbaldehyde
4-Vinylbiphenyl
5-alpha-Dihydroprogesterone
2-CHLORO-5-NITROBENZAMIDE
4-N-HEXYLOXYBENZALDEHYDE
PolydiMethylsiloxane
2-Methoxyphenylboronic Acid
1-BroMooctane
LithiuM Trifluoro(trifluoroMethyl)borate - DiMethyl Carbonate CoMplex
t-Butyl 4-hydroxy-7,8-dihydropyrido[4,3-d]pyriMidine-6(5H)-carboxylate
3-Methoxy-4-(Methoxycarbonyl)phenylboronic acid pinacol ester, 95%
CARMINE
3,4-MethylenedioxycinnaMic Acid
Aluminum
nefopam hydrochloride
Potassium isopropoxide
2,4-Dichloro-5-pyrimidinecarbonyl chloride
AQP-1 ELISA Kit
RasGAP ELISA Kit
Human PCDHα11 ELISA Kit
Human DPEP3 ELISA Kit
CRP ELISA Kit
Aβ1-42 ELISA Kit
G-CSFR ELISA Kit
Elastase-1 ELISA Kit
Human SAP ELISA Kit
INB ELISA Kit
Kligler Iron Agar
Skimmed Milk Sucrose Peptone Medium Base
Zinc 2,4-Pentanedionate Monohydrate
AluMinuM fluoride, anhydrous, 99.99% (Metals basis)
5,5-DiMethyl-1,3-dioxane-2-butanal, 96%
2-FLUORO-3-METHYLPYRIDINE-5-BORONIC ACID
altretamine
3-benzylpiperidine
12-MOLYBDOSILICIC ACID HYDRATE
DL-homocysteic acid
1-Hexyl-3-methylimidazolium tetrafluoroborate
10-CHLORO-9-ANTHRALDEHYDE
2,3-Dihydro-1H-Pyrrolo[2,3-B]Pyridine
Gold(III) hydroxide, Au 79% Min
3-Chloro-4-Methylbenzenesulfonyl chloride, 99%
2-AMinobenzeneboronic acid pinacol ester
2-Hydroxy-4-Methyl-5-nitropyridine
TETRANITROPHENOLSULFONPHTHALEIN
N-EthylMorpholine
2-Ethyl-4-Methyl thiazole
1-AMinonaphthalene
1-(4-Bromo-2-fluorobenzyl)
Silver powder (99.9%)
2-Chloro-6-methoxyisonicotinamide , 95%
Methyl 3-bromo-5-tert-butylbenzoate
