SW 1783[SW-1783,SW1783]细胞：人脑星形胶质细胞系热卖

SW 1783[SW-1783,SW1783]细胞：人脑星形胶质细胞系热卖 货号：GD-C7120482
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
PlatinuM 30 wt% RhodiuM wire, 0.076MM (0.003in) dia, ISA Type B Standard Grade TherMocouple
4-Oxoisophorone
4-(4-BroMo-1H-pyrazol-1-yl)-6-chloropyriMidine
2,5-DibroMonitrobenzene
AMMoniuM bicarbonate
5-BroMo-4-(4-ethylphenyl)pyriMidine
1,2:3,4-DIBENZANTHRACENE
Silica Gel (Spherical, 60micro m) [for Flash Chromatography]
N-(2-Methoxy-5-nitrophenyl)maleimide
3-Vinyl heptamethyl trisiloxane
2-bromo-4-methylbenzothiazole
9-Borabicyclo[3.3.1]nonane
Nickel fluoride
Chloromethyl silane
9,10-Dihydrophenanthrene
5-Chloropyridin-3-ol
4-(DiMethylaMino)benzenethiol
Methyl 5-broMopyrazine-2-carboxylate
2-Methoxypyrazine, 95%
Tetraethyleneglycol DiMethacrylate
Bis[(2S,3aR,4S,7aR)-octahydro-7,8,8-triMethyl-4,7-Methanobenzofuran-2-yl] Ether [for Optical Resolution]
3-Acetylindole
1-(trimethylsiloxy)-1,3-butadiene
dirhenium heptaoxide
2-Aminopyridine
1,4-Cyclohexanediol
3,5-Dichloro-4-hydroxypyridine
4,4-Diiodobiphenyl
5-Hydroxy-3,4-diMethoxybenzoic acid, 97+%
CinnaMaMide, predoMinantly trans, 97%
9-Carbazoleacetic acid
9-BroMoanthracene
4-BroMo-2-(trifluoroMethoxy)benzenesulfonyl chloride
N-Phenyl 4-broMobenzaMide
CinnaMyl propionate
N-(tert-Butoxycarbonyl)-(S)-(+)-3-pyrrolidinol
4,5-Dichlorophthalonitrile
2-Hexanone
1-(Methoxymethoxy)naphthalene
1,4-Dibutylbenzene
2,3-Dibromothiophene
3-CHLORO-4-HYDROXYBENZOIC ACID
2-(4-Methylbenzoyl)benzoic acid
Pyridinium toluene-4-sulphonate
2-AMino-5-iodopyridine
SalicylaldoxiMe
Tetrakis(triphenylacetato)dirhodiuM(II) DichloroMethane Adduct
1-(1H-Pyrrol-2-yl)ethan-1-one, tech
Tris(triMethylsilyloxy)ethylene, 95%
1-Chlorobutane
2,2,2-Trifluoroethyl Butyrate
2,4-Difluorobenzonitrile
BPTA
ACIDOL-4BENZYLOXYCARBONYL-AMINO-2HYDROXY BUTYRIC
1-(4-Chlorobenzenesulfonyl)urea
BETA-PHENYLPROPIOPHENONE
3-CHLORO-1H-INDAZOLE
Diphenylacetylene
1-(4-Chlorophenyl)-4,4,4-trifluoro-1,3-butanedione
2-Chloro-5-aminopyrimidine
1-Pyridin-4-yl-piperidine-4-carboxylic acid
tert-Butyl isocyanate, 97%
2,6-Dichloroquinoline-3-carboxaldehyde
1,2-DIIODOETHANE
N-Vinylcaprolactam
D-(+)-TURANOSE
Tetrabutyl titanate
5-Cyanoindole
1-Chloro-3-fluorobenzene
Methyl 4-Iodosalicylate
4-(4-Chlorophenyl)-2-Methylthiazole
2,4,6-Trifluorophenylboronic acid, 95%
1,4-Diiodobutane
1,4-Benzodioxane-5-carboxylic Acid
2-(3-bromophenyl)-1,3-dioxolane
2-butylcyclohexanol
rifampicin
Phenylalanine,2,4-dichloro-
Grb10 ELISA Kit
MGMP ELISA Kit
Human Free erythrocyte proloporphyrin,FEP ELISA Kit
Human ADP ELISA Kit
ET ELISA Kit
βOHB ELISA Kit
Hsp-60 ELISA Kit
Human PDIA3 ELISA Kit
Schistosoma ELISA Kit
APOC4 ELISA Kit
McBride Agar Base,Modified
Trypticase Soy-Yeast Extract Broth
PlatinuM(II) 2,4-pentanedionate, Pt 48.0% Min
AMMoniuM hexafluorozirconate
5-(4-Fluorophenyl)-1H-tetrazole, 97%
2-Butynal diethyl acetal, 98%
3-Acetylindole
3,4,5-Trihydroxybenzaldehyde
Sodium aluminate
benzylurea

2,6-Pyridinedicarboxylic acid chloride
Methyl anthranilate
Silica Gel
Nickel(II) perchlorate hydrate, 99.998% (Metals basis)
1-Boc-5-Methylindole-2-boronic acid, 97%
4-Hydroxybenzophenone
Tripropylphosphine
4-METHYL-3-(TRIFLUOROMETHYL)BENZYL BROMIDE
1,1-Difluoroethylene
2-ACETAMIDOPYRIDINE
2-(2-AMinoethyl)isothiourea dihydrobroMide
1-(4,4'-Dichlorobenzhydryl)
1,3-Dihydro-1-methyl-2H-benzimidazol-2-one
Ethyl 2-amino-4-(trifluoromethyl)-5-thiazole-carboxylate
3-Methyl-2-butanone, 98%