A-431 Cell：人表皮癌细胞系(来源于ATCC等知名细胞库)

A-431 Cell：人表皮癌细胞系(来源于ATCC等知名细胞库) 货号：GD-C6819123

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Hydroxy-5-nitrobenzaldehyde, 98%
EDTA tripotassiuM salt
9,9'-DIXANTHYLIDENE
(1S,2S)-trans-2-AMino-1,2-dihydro-1-naphthol hydrochloride
N-VinylcaprolactaM
4'-CHLORO-2,2,2-TRIFLUOROACETOPHENONE
(E)-5-(2-BroMovinyl)uracil
5-Bromo-2-chloro-3-nitropyridine, 98%
Diethyl (N-Methoxy-N-methylcarbamoylmethyl)phosphonate
2-Methoxy-6,7,8,9-tetrahydro-benzocyclohepten-5-one
Methyl 3-methylphenylacetate, 98%
2,4,6-TRICHLOROANISOLE
2,3-Cyclohexeno pyridine
2-BROMOBENZHYDRAZIDE
4-(Methylthio)-6-(2-pyridyl)-1,3,5-triazin-2-aMine
(1R,2R)-(+)-1,2-DiphenylethylenediaMine
2,3-Dihydro-2-spiro-7'-[8'-iMino-7',8'-dihydronaphthalen-1'-aMine]periMidine
Vinyl 10-Undecenoate (stabilized with MEHQ)
Triphosphopyridine nucleotide, sodiuM salt, hydrate
Safflower Oil, USP
TriMethylpropylaMMoniuM Bis(trifluoroMethanesulfonyl)iMide
cyclohexyl acetate
DL-2-Pentanol
Aminomalonate
2,6-Dichloro-4-fluorophenol
6-METHYL-1,2,3,4-TETRAHYDROQUINOLINE
4-CYANOBENZOPHENONE
N4-Acetyl-2-Deoxycytidine
1,4-Cyclohexanedione bis(ethylene acetal), 99%
N-(2-Carboxyphenyl)glycine, 97%
Boldenone Solution
Cytidine-5'-diphosphate disodiuM salt
N,N'-1,4-PHENYLENEDIMALEIMIDE
PHENYLSILANE
BENZENESULFONYL FLUORIDE
2-ISOPROPENYLNAPHTHALENE
3'-Acetyl-biphenyl-3-carbaldehyde
5-Chloro-2-fluoro-4-methylpyridine
5-Chloro-2-methylanisole
4-[(Methylsulphonyl)amino]benzoic acid
5-Methoxy-3,4-dihydro-2H-naphthalen-1-one
Butylate
MAGNESIUM TRISILICATE
1,4-Butanediol dimethacrylate
Butyltin trichloride
delta-Octanolactone
Bis(trifluoro-2,4-pentanedionato)cobalt(II) Hydrate
2-(4-Chlorosulfonylphenyl)ethyltriMethoxysilane, 50% solution in dichloroMethane
Pyridine-3-boronic acid, 95%
o-Cresol
2-Chloro-4-fluorobenzoic Acid
4,5-difluoro-2-nitroaniline
pentadecylmagnesium bromide
boc-O-benzyl-L-tyrosine hydroxy succinimide ester
2-chloro-3-methylpyridine
Potassium sorbate
5-ForMyl-2-furanboronic acid MIDA ester
BIS(4-METHOXYPHENYL) CARBONATE
(R)-(-)-TetrahydrofurfurylaMine
Benzo[c]cinnoline
Inositol, 98+%
Aflatoxin B1
Bromohydroquinone
Oxyclozanide
17alpha-Hydroxy-yohimban-16alpha-carboxylic acid methyl ester
METHYLLITHIUM
N-(2-Acetamido)iminodiacetic acid
1-Dodecanesulfonic acid
1-Allyl-3-MethyliMidazoliuM chloride
PotassiuM 1,1,2,2,3,3-Hexafluoropropane-1,3-disulfoniMide
Boc-Orn-OH
4-(ChloroMethyl)-2,5-diMethyl-1,3-oxazole, 97%
1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE
3,5-DiMethylanisole
2-Aminophenol-5-sulfamide
2,3,4-TRIHYDROXYDIPHENYLMETHANE
2,2-Bis(hydroxymethyl)propionic acid
S-Propionylthiocholine iodide
AGEs ELISA Kit
Mouse COL10 (Collagen Type X) ELISA Kit
Human APOL3 ELISA Kit
Human DEFβ2 ELISA Kit
Chicken Complement 3 split product,C3SP ELISA Kit
TLR-9/CD289 ELISA Kit
E2 ELISA Kit
PYGB ELISA Kit
Human Angiopoietin 4,ANG-4 ELISA Kit
ANG ELISA Kit
BG-11 Medium
Antibiotic test medium No.1 (high pH) (pH7.8-8.0)
LithiuM isopropoxide, 99.9% (Metals basis)
CalciuM granules, redistilled, -16 Mesh, 99.5% (Metals basis)
5-Chloro-2-fluoroaniline, 97%
2-Fluoro-6-methylpyridine
cyclohexyl acetate
acid yellow 159
dextran sulfate so-salt from leuconostoc ssp.
2-Fluoro-4-methylphenol

1-Acetylpiperidin-4-amine
3-Fluoro-4-methylbenzonitrile
Cobalt(II) fluoride, ultra dry, 99.99% (Metals basis)
5-Benzyloxy-2-nitrotoluene, 98%
2,3-Difluoro-4-Methoxybenzyl broMide, 97%
N-Acetyl-L-tryptophan
2-[2-(2-Azidoethoxy)ethoxy]ethanaMine
1,3,5-TRIVINYL-1,3,5-TRIMETHYLCYCLOTRISILAZANE
Genet extract
Methyl 2-hydroxy-1-naphthoate, 98%
2-BroMo-5-(trifluoroMethyl)phenylacetonitrile, 97%
4-Iodophenol
3-Bromo-4-fluorophenylacetic Acid
4-Acetamido-2,2,6,6-tetramethyl-1-oxopiperidinium Tetrafluoroborate
3-Methoxyphenylmagnesium bromide, 1M solution in THF/toluene, AcroSeal