HCC-1833 Cell：人肺癌腺癌细胞系(来源于ATCC等知名细胞库) 货号：GD-C6820013
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-(4-MorpholinylMethyl)thiophene-2-boronic acid pinacol ester, 97%
3-Chloro-o-xylene
5-Chloro-2,3-diMethoxypyridine
VINYL IODIDE
2-Methoxyphenothiazine
Cyclohexen-1-ylboronic acid
Ethyl 4-(MethylaMino)piperidine-1-carboxylate
4-ethynylbenzaldehyde(SALTDATA: FREE)
4-(tert-Butyl)-2-methyl-1,3-thiazole, 97%
5-Bromo-2-methoxynicotinonitrile 98%
2,4-Dibromo-1-naphthol
Fmoc-N-methyl-L-glutamic acid 5-tert-butyl ester
2-NONYNE
1,3-Dimethyl-1H-pyrazole-5-carbonyl chloride
PANOSE
Citric acid
trans-1,3-Diphenyl-2-propen-1-ol
N-t-Butyl 3-broMo-4-MethylbenzenesulfonaMide
TetraMethylaMMoniuM acetate hydrate, 95%
Ninhydrin
Triethoxy(1H,1H,2H,2H-nonafluorohexyl)silane
ethyl linoleate
5,6-dimethoxypyrimidin-4-ylamine
n-Nonanoyl Chloride
Di-N-pentyl phthalate
(2-Aminopyridin-3-yl)methanol
2,5-DICHLOROFLUOROBENZENE
2-Chloro-N-(3-fluorobenzyl)aniline, 97%
4-MethoxybenzylaMine, 98+%
5-Chloro-2-hydroxybenzaMide, 97%
4,4'-DiMethyl-2,2'-dipyridyl
2-(Dicyclohexylphosphino)-1-(2-Methoxyphenyl)-1H-pyrrole
SILVER P-TOLUENESULFONATE
SodiuM 2,3,5,6-Tetrafluoro-4-hydroxybenzenesulfonate
2-Methoxy-1,4-naphthoquinone
1,3-DiiMinoisoindoline
4-Methyl-2-[1-(tert-butoxycarbonyl)piperid-4-yl]-1,3-thiazole-5-carboxylic acid , 97%
Phenoxyacetyl Chloride
2-(3-chlorophenoxy)-2-methylpropanoic acid(SALTDATA: FREE)
2-(2-Naphthyl)indole
Ractopamine
2,3-Dibromo-1,4-butanediol
3-Methyladipic acid
1-Phenyl-1,3-propanediol
Guanidine thiocyanate
5-BroMoacenaphthene
Ethyleneglycol-1,2-bis(succiniMidyl 3-oxypropionate)
Adenosine 3',5'-cyclic Monophosphate, 99+%
1,1-DiMethylbiguanide hydrochloride, 97%
ThioseMicarbazide
Diethylene Glycol MonoMethyl Ether Methacrylate (stabilized with MEHQ)
3,5-Dichlorophenol
4-Ethoxy-cyclohexanecarboxylic acid
4-Bromo-2-chlorotoluene
tert-butyl P,P-dimethylphosphonoacetate
3,4-Dichlorobenzyl bromide
2-Butoxy-5-fluorophenylboronic acid
t-Boc-aMinocaproic-N-hydroxysucciniMide
(4bS)-trans-8,8-TriMethyl-4b,5,6,7,8,8a,9,10-octahydro-1-isopropylphenanthren-2-ol
5-Bromo-2-fluoroaniline 98+%
Selenium(IV) sulfide, min. 94%
4-Bromo-1-methyl-1H-pyrazole
4-Chloro-2-methylacetanilide, 95+%
Calcium stearate
5-Amino-2-chlorobenzoic acid
4-(TRIFLUOROMETHYLTHIO)BROMOBENZENE
2-(7-Aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate
3-BroMopyridine
1,2-DiaMinopropane
1-Ethyl-3-Methylcyclohexane (cis- and trans- Mixture)
N'-(4-Chlorobenzoyloxy)-4-nitrobenziMidaMide
(R)-alpha-Methylphenylalanine hydrate, 98%, 98% ee
BUTYLATE
4-tert-Butylpyrocatechol
6-Methyl-2-pyridinecarboxaldehyde
Hydrogen sulfide
octadecylamine hydrochloride
3-Chlorobenzoyl chloride
VEGF ELISA Kit
CEA/CD66 ELISA Kit
Human Adiponectin,Acrp30 ELISA Kit
Human CNX ELISA Kit
VDBP ELISA Kit
Rat GATA5 (GATA Binding Protein 5) ELISA Kit
Monkey Pg (Progesterone) ELISA Kit
Human FGF2/bFGF ELISA Kit
Human thymic stromal lymphopoietin,TSLP ELISA Kit
AST ELISA Kit
RPF Agar Medium Base
Tinsdale Agar Base
Indoline
CITRATE HYDRATE
5-AMinoindane, 98+%
2-Methyl-3-pentanol, 99%
ethyl linoleate
Styrene, 1,3-butadiene polymer
dimethyl 1,2,2-trimethylcyclopentane-1,3-dicarboxylate
3-hydroxynaphthalene-2,7-disulphonic acid
2,4-DIHYDROXY-5-TERT-BUTYLBENZOPHENONE
1,1,3,3-TETRAISOPROPYLDISILOXANE
Sulfaguanidine
Cobalt(II) perchlorate hexahydrate, 99.999% (Metals basis)
Di-n-butylgerManiuM dichloride, 97%
Ethyl diphenylphosphinite
3,3-Diethoxyacrylic Acid Ethyl Ester
Theophyllol
TETRAETHYLAMMONIUM FLUORIDE TETRAHYDROFLUORIDE
EOSIN B
3-BroMo-5-Methyl-2-(3-Methoxybenzyloxy)phenylboronic acid
Disialoganglioside GD1a ,95%
Polyethyleneglycol 400 monooctylether
L-Ethionine
(1,3-Dimethyl-1H-pyrazol-4-yl)methylamine
