HCC-2108 Cell:人肺癌腺癌细胞系(来源于ATCC等知名细胞库) 货号:GD-C6820014
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明:请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式:T25培养瓶(一般是贴壁细胞使用,悬浮细胞也可以)、离心管(悬浮细胞使用多,当然,有些公司也会用这种离心管形式发贴壁细胞,但是,长期经验来说,贴壁细胞容易发生形态变化,估计是细胞培养空间狭小,血清不足导致(Z主要是怕运输延误导致这些情况发生),个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用,主要是冬天天气冷,温度低于4℃的地方,都要考虑冻存细胞)。T25培养瓶:是用T25细胞培养瓶直接发货的形式。收到细胞后,拆开包装T25培养瓶的自封袋,不拆封口膜,表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上,再处理细胞。首先将T25中培养基取出装好备用,如细胞密度高于80%,可以直接消化传代,相反则加入5-6ml培养基放回培养箱继续培养即可。离心管:是用15ml离心管发货的形式,只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上,再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶,可以暂时T75培养瓶,加入10-15ml培养基,建议10ml培养基,也可以使用T175培养瓶,加入50-60ml培养基,培养瓶越大,需要的培养基越多。当然如果刚接收到细胞密度不够的话,Z好不用大瓶子,先用小瓶子养起来再换大的,不然会长得很慢,严重的,会导致细胞死亡等危险),平衡完成后,离心(1000rpm,3min)收集细胞,弃上清,用新的培养基重悬细胞并接种至培养瓶或皿中,放回培养箱继续培养。干冰:是用本公司冻存液冻存细胞后,直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,6-Difluoro-4-iodophenol, 99%
MethylMercury chloride
2,6-Dichloro-5-fluoro-3-pyridinecarbonitrile
CyclohexylMethanethiol acetate
4-CHLORO-2-(TRIFLUOROMETHYL)PHENYL ISOCYANATE
Bis(tetraethylaMMoniuM) bis(2-thioxo-1,3-dithiole-4,5-dithiolato)zincate, 97%
1,2,3,6,7,8-HEXAHYDROPYRENE
3-Hydroxypyridine
Pentamethyliodobenzene
4-[(Methylcarbamoyl)amino]benzeneboronic acid, pinacol ester 95%
(Phenyldimethylsilyl)methyl methacrylate(Methacryloxymethyl phenyldimethylsilane)
2,5-Dimethyl-2,5-hexanediol
1-BROMO-3-ISOPROPOXYBENZENE
IODINE MONOBROMIDE
Methyl 6-bromo-2-naphthoate
DansylaMide
2,2-DiMethylhexanoic Acid
t-Butyl 4-broMo-1-naphthoate
TetraMethylaMMoniuM broMide, 98%
Nisin
4-BroMobenzyl Cyanide
ammonium oleate
2-(1-Naphthoyl)-benzoic acid
dec-1-yne
A-naphthyl red hydrochloride
1-(4-Bromophenyl)octane
TRIETHANOLAMINE LAURYL SULFATE
Isopropyl BroMide
LithiuM hexafluorophosphate, 98%
2-(4-Methyl-5-thiazolyl)ethyl acetate, 98+%
4-Hydroxy-1-indanone
p-Fluoro benzaldehyde
2-OCTYNOIC ACID
SODIUM 1-UNDECANESULFONATE
5-AMinooxindole
2-Hexyne, 98+%
5-Fluoro-2-(trifluoromethyl)benzaldehyde 97%
5-(3,4-Dichloro-phenyl)-1 H -pyrazole-3-carboxylic acid
[4-(5-methyl-1H-tetrazol-1-yl)phenyl]acetic acid(SALTDATA: FREE)
2-Amino-3-bromobenzotrifluoride 98%
1,4-Butane sultone
BIS(4-NITROPHENYL) SULFIDE
Oxazole
HOLO-TRANSFERRIN
D-Xylopyranose
2-AMino-6-Methoxypurine
5-BroMo-2-hydroxy-4-trifluoroMethylpyridine
3-Methyl-2-oxobutanoic acid, sodiuM salt, 98+%
3,5-Dichloro-2-hydroxybenzenesulfonyl chloride, 90-95%
CELLULASE
4-Methyl-2-phenyliMidazole
1-Bromopropane
(S)-(+)-N,N-Dimethyl-3-Napthtoxy-(2-Thiophene) Propylamine Oxalate
2-amino-3-acetylpyridine
2-Chloro-3,4-diaminopyridine
(3-CHLORO-PHENYL)-METHANESULFONYL CHLORIDE
3-Chloroperoxybenzoic acid
2-AcetaMido-5-fluorophenylboronic acid, pinacol ester
Tetrakis(triphenylphosphine)platinuM
4-Methyl-4-cyclohexene-1,2-dicarboxylic anhydride
Imidazo[1,2-a]pyridin-6-ylmethanol, 97%
Nimodipine
Poly(hexadecyl methacrylate), 28% solution in toluene, average M.W. 190.000
Methyl 4-(butyrylamino)-3-methyl-5-nitrobenzoate
Hexabromocyclododecane
1-PHENYLISATIN
Guanidine nitrate
Methyl chloroacetate
1,2-DibroMo-3-chloropropane
2-Methoxy-4-Methylpyridine
4,5-DiMethyl-4H-1,2,4-triazole-3-thiol
Bis(1,5-cyclooctadiene)iridiuM(I) tetrafluoroborate, 98%
POTASSIUM HYDROGEN D-TARTRATE
9-AnthrylMethyl Acrylate
Methyl 4-(aminomethyl)benzoate
O-6-ethoxy-2-ethylpyrimidin-4-yl O,O-dimethylphosphorothioate
diboron calcium tetraoxide
Praseodymium acetate
PROCR ELISA Kit
Mouse carcinoembryonic antign,CEA ELISA Kit
Adiponectin ELISA Kit
CNX ELISA Kit
MT ELISA Kit
GATA5 ELISA Kit
P ELISA Kit
Human FGF21 ELISA Kit
Human TSLP ELISA Kit
HBP ELISA Kit
Chapman Agar Medium
Blood Agar Base with Potassium Tellurite
Indole-7-carboxylic acid, 97%
Alverine citrate
5-AMino-1H-indazole, 98%
2-Methyl-3-Thiophenecarboxylic Acid, Ethyl Ester
ammonium oleate
Resins, terpene
beta-D-Allose
2-(4-thiazolyl)benzimidazole
ETHYL 2-CYANOACETOACETATE
1,3-DIAMINO-2-PROPANOL-N,N,N',N'-TETRAACETIC ACID
Boc-Osu
3-AMino-4-chloro-1H-indazole, 97%
Polyethylene, chlorinated
Ethylene glycol diMethyl ether
Diethyl (3-Chlorobenzyl)phosphonate
Hydrogen broMide
4-(TrifluoroMethylthio)benzaMide
CHROMIUM(III) NITRATE NONAHYDRATE
Benzoin resin absolute
Oxamic hydrazide, 99%
2-Methyl-5-nitroindole, 95%
2,3-Difluoro-6-nitroaniline
Isoquinoline-8-carbonitrile