DV-90 Cell：人肺癌腺癌细胞系(来源于ATCC等知名细胞库) 货号：GD-C6820009
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,4-Dichlorobenzyl chloride, 98%
Triethoxysilane
Methyl Heptafluoroisobutyrate , 98%
2-[1-(MercaptoMethyl)cyclopropyl]acetic acid
Ethyl cellulose
1-BroMo-4,5-diMethoxy-2-nitrobenzene
5-Methyl-2-(trifluoroMethyl)benzonitrile, 97%
Pyrazine-2-carboxaldehyde
4-(4-chloro-6-ethylpyrimidin-2-yl)morpholine(SALTDATA: FREE)
3-Methyl-3-heptanol
1,2-Dimethylnaphthalene
3-(4-METHOXYPHENYL)-1H-PYRAZOLE-4-CARBALDEHYDE
ZINC TUNGSTATE
4-Chloro-2-(trifluoromethyl)benzoic acid
DL-tert-Leucine
2-Methylsulphonylaniline hydrochloride
(S)-3,3'-DibroMo-2,2'-diMethoxy-1,1'-binaphthyl
N-Butyl 4-BroMo-3-MethoxybenzaMide
Tetrakis(diMethylaMino)zirconiuM
Nile Blue A
(S)-2-(AMinoMethyl)-1-(tert-butoxycarbonyl)pyrrolidine
Glutaronitrle
4-AMINO-N-(6-CHLORO-5-METHOXY-4-PYRIMIDINYL) BENZENESULFONAMIDE
trans-2-penten-1-al
Dimethyl phthalate
METHANOL-D4
HECTORITE
5-Methoxyindole
3-FluorobenzenesulfonaMide, 97%
2-AMino-5-fluorophenol, 97%
2-AMino-4-tert-butylphenol
AMMoniuM nitrate
PYRAZINE-2-CARBOTHIOAMIDE
SodiuM 2-biphenylate
3-Fluoro-4-aMinobenzonitrile
4-(4-Methoxyphenyl)butyric acid
Ethyl 3-oxo-3-(2,4,5-trifluorophenyl)propanoate
6-(Dibutylamino)-1,3,5-triazine-2,4-dithiol
4-Methoxy-2-(trifluoromethyl)benzonitrile
Diacetato[(S)-(-)-2,2'-bis(diphenylphosphino)-5,5',6,6',7,7',8,8'-octahydro-1,1'-binaphthyl]ruthenium(II) Ru(OAc)2[(S)-H8-binap]
3-Amino-4-chlorophenol
TRILINOLEIN
1-(2-AMINOETHYL)-4-BENZYL
carbonic acid: cyclohexanamine
Ethylxanthic acid potassiuM salt
4-Chloro-3-nitrocinnaMic acid
Methyl Tricosafluorododecanoate
N-(2-Fluoro-4-iodophenyl)acetaMide
3,5,7-TriMethyladaMantane-1-carboxylic acid, 97%
Toradol
Acid Red 27
1-(4-Fluorophenyl)-1-Methyl-Ethylamine
fibrinopeptide B human
3-[[4-[(2,6-dichloro-4-nitrophenyl)azo]phenyl]ethylamino]propiononitrile
2-Isopropylthioxanthone
Isopropyl isothiocyanate
GerMane
DisodiuM 1,3-Benzenedisulfonate , 55%
4,4',4'',4'''-TETRA-TERT-BUTYLPHTHALOCYANINE COPPER
Isopropyltriphenylphosphonium Iodide
3-Fluoro-4-[(2-hydroxyethyl)carbamoyl]benzeneboronic acid 98%
3-Oxo-cyclopentanecarboxylic acid ethyl ester
Scandium(III) nitrate pentahydrate (99.9%-Sc) (REO)
3-Nitrocinnamic acid
5-[2-Cyclopropyl-1-(2-fluorophenyl)-2-oxoethyl]-5,6,7,7a-tetrahydrothieno[3,2-c]pyridin-2(4H)-one
1-BROMO-2,4,6-TRICHLOROBENZENE
4-Trifluoromethylphenol
6-Methoxy-1-indanone
1,2-Cyclododecanediol (cis- and trans- Mixture)
3-(Piperidino)phenylboronic acid
HexaMethylphosphorous triaMide, 97%
N-[(1,3-DiMethyl-1H-pyrazol-5-yl)Methyl]-N-MethylaMine, 97%
4-(Methylthio)benzyl Alcohol
MethyltrioxorheniuM(VII)
3-methoxypropionic acid
2-Vinylpyridine
O-(1,2-dihydro-2-oxo-1-pyridyl)-bis(te. methylene)uron. pf6
HECAMEG
Porcine VEGFR1/Flt1 (Vascuoar Endothelial Growth Factor Receptor 1) ELISA Kit
NT-ProBNP ELISA Kit
Human adiponectin，ADP ELISA Kit
Human CAPS ELISA Kit
CKMT1A ELISA Kit
Rat GATA binding protein 4,GATA4 ELISA Kit
Monkey Insulin,INS ELISA Kit
Human FGF17 ELISA Kit
TARC/CCL17 ELISA Kit
GPBB ELISA Kit
Pseudomonas Cepacia Agar Base
Bordet-Gengou Agar Medium Base
IndoMethacin, 98%
COPAIBA OIL
5-Phenyl-1,3,4-thiadiazole-2-thiol, 96%
2-Methyl-3-Nitropyridine
Glutaronitrle
Poly(vinyl formal)
D-Lysine Hydrochloride
kanamycin sulfate mixture of components A
1,2-Dibromobenzene
THIAZOLIDINE-2-CARBOXYLIC ACID
D-GlucosaMine hydrochloride
2-(1-Naphthyl)pyrrolidine, 95%
Methyl 4-azaindole-2-carboxylate, 95%
4-Methoxybenzenesulfonyl chloride, 98+%
(S)-Ru(OAc)2(T-BINAP)
2-AcetaMido-2-deoxy-D-glucose
6-AMino-2-Mercaptobenzothiazole , 95%
Tetrahydro-2-Methyl-3-thioxo-1,2,4-triazine-5,6-dione
N-DODECYL CYANIDE
BIS(PENTAMETHYLCYCLOPENTADIENYL)IRON
4-Fluoro-2-(trifluoromethyl)cinnamic acid
2-(Trifluoromethyl)benzoic acid hydrazide 95%
1,4-Bis(bromomethyl)naphthalene (contains ca. 23% isomer)
