MEC-1 Cell：人粘液表皮样癌细胞系(来源于ATCC等知名细胞库)

MEC-1 Cell：人粘液表皮样癌细胞系(来源于ATCC等知名细胞库) 货号：GD-C6818848

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2,4-DichlorobenzylaMine, 98%
Triclosan
4-(2-BroMophenylsulfonyl)Morpholine
3,5-DiaMinobenzyl alcohol dihydrochloride
4-Ethynylpyridine
Mandelonitrile
3-Methylsulfinylphenylacetic acid
2-Chloro-5-hydroxybenzoic acid 98%
4-Methyloxazole-5-carbonyl chloride , 97%
3-Fluoro-4-nitrobenzyl alcohol 99%
1-(Bis-dimethylamino-methylene)-5-chloro-3-oxy-1H-benzotriazol-1-ium tetrafluoroborate
ETHYL (METHYLTHIO)ACETATE
Benzyldimethylcarbinyl butyrate
Cesium formate
2-Hydroxy-5-trifluoromethylpyridine
2-Chlorophenylacetic acid
4-DiethylaMino-2-butyn-1-ol
N-t-Butyl 4-broMo-3-MethoxybenzaMide
Tetrakis(triphenylphosphine)nickel(0), 95%
Nile Red
2,6-DiaMinoanthraquinone
Calcium Formate
4,6-dichloro-5-methoxypyrimidine
2-Hexyn-1-ol
Dipropyl phtalate
3,5-DIMETHYL-4-OCTANONE
Pyridine-2-carboxiMidaMide hydrochloride
Isopentyl butyrate, 98%
Methyl 3-cyano-4-Methoxybenzoate, 97%
Methyl 3,5-dibroMo-4-Methylbenzoate, 98%
2'-Hydroxy-4'-Methoxyacetophenone
Ethyl 3-hydroxybutyrate
Methyl 4-hydroxy-8-Methylquinoline-2-carboxylate
SodiuM 2,4-pentanedionate hydrate, 95%
METHYLALUMINUM BIS(2,6-DI-TERT-BUTYL-4-METHYLPHENOXIDE)
1,5,9-TRIAZACYCLODODECANE
Bicine, for analysis, 99+%
Perseitol ,98%
5-Fluoro-2-methoxybenzaldehyde
2-tert-Butylsulfanyl-benzoic acid
5,8-Dihydroxy-2-[(1R)-1-hydroxy-4-methyl-pent-3-enyl]naphthalene-1,4-dione
Triethoxypropylsilane
Potassium chloride
Sulfaguanidine
5-BroMo-1-pentene
(R)-4-Benzyloxy-1,3-butanediol
3-(Cyclopentyloxy)-5-Methylphenylboronic acid
cis-2-(tert-ButoxycarbonylaMino)-1-cyclopentanecarboxylic acid, 98%
3-BroMochlorobenzene, 99%
N,N-DiethylchloroacetaMide
6-Hydroxynicotinic Acid
alpha-cyanocinnamic acid
2-Amino-2'-chloroacetophenone
[1,1'-Biphenyl]-4-carboxylic acid [3aR-[3aa,4a(E),5b,6aa]]-4-(4,4-difluoro-3-oxo-1-octenyl)hexahydro-2-oxo-2H-cyclopenta[b]furan-5-yl ester
Methyl 1,4-dihydroxy-2-naphthoate
N-Methylaminopropyltrimethoxysilane
3-Picolyl chloride hydrochloride
(S)-(?)-N-tert-Butyl-1,2,3,4-tetrahydro-3-isoquinolinecarboxaMide
(R)-(-)-2-Methyl
2-Ethyl-5-methylthiophene ,99%
3'-Fluoro-4'-hydroxyacetophenone
Chloroxazone, 98%
4-Methoxybutyl Acetate
Sodium dodecyl sulfate
4-(2-Aminoethyl)benzenesulfonamide
Pentyl butyrate
(S)-(-)-2-Phenylglycinol
6-Nitroveratraldehyde
1,2-Di(2-pyridyl)ethylene
4-BroMo-2-nitrophenylboronic acid
Acetal, 99%, pure
(BroMoMethyl)cyclopropane, 96%
TriacetoxyMethylsilane
5-Decyne
3-Hexanone
4-Benzyloxycarbonylamino-N-Boc-piperdine
D(+)-Methioninol
ethyl 3-naphthalen-1-yl-3-oxopropanoate
VEGF-B ELISA Kit
CEF ELISA Kit
Human ADP ELISA Kit
Human calnexin，CNX ELISA Kit
PGC ELISA Kit
GATA4 ELISA Kit
IGF-1 ELISA Kit
Human FGF19 ELISA Kit
Human TARC/CCL17 ELISA Kit
GLTP ELISA Kit
Vogel-Johnson Agar Base
Cellulose Decomposing Microorganisms Medium
Indometacin
DIETHYLCARBAMAZINE CITRATE
5-Phenyl-1,3,4-oxadiazole-2-thiol, 98%
2-Methyl-3-nitrobenzyl chloride
Calcium Formate
Polyvinylpyrrolidone
estrone sulfate
L-Ascorbic Acid Sodium Salt

2,2'-BITHIOPHENE
3-Fluoro-5-(trifluoromethyl)benzoic acid
Allyl Sulfide
^a,^a,^a',^a'-TetrabroMo-o-xylene, 97%
HarMine, 98+%
PotassiuM phosphate Monobasic
3-AMinoMethyl-3,5,5-triMethylcyclohexylaMine
3,5-Dichloroiodobenzene
2-FLUOROPHENYLUREA
5-(ChloroMethyl)-3-(3-nitrophenyl)-1,2,4-oxadiazole
TRIETHYL 3-PHOSPHONOPROPIONATE
5-Chloro-2-hydrazinopyridine
Cycloheptyl isothiocyanate
Ethyl 4-methyl-4-pentenoate, 95%
Nitrilotris(methylene)triphosphonic acid, 50 wt% solution in water