NCI-H1781[H1781]人细支气管肺泡癌细胞系完全培养基

NCI-H1781[H1781]人细支气管肺泡癌细胞系完全培养基 货号：GD-CP110476
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
p-Toluic acid, 98%
1-Naphthyl phosphate MonosodiuM salt Monohydrate
1,1-DIMETHYLGUANIDINE SULFATE
3-CHLORO-6-FLUOROBENZOTHIOPHENE-2-CARBONYL CHLORIDE
DiMethyl Methylphosphonate
Phenyl 1,4-dihydroxy-2-naphthoate
3-(BOC-AMINO)-1-PROPANOL
4-Bromo-2-fluorobiphenyl
Collodion, flexible
3-Cyanophenylacetic acid, 95%
Bis(tetrabutylammonium) Bis(1,3-dithiole-2-thione-4,5-dithiolato)zinc Complex [Organic Electronic Material]
3,5-Bis(1,1-dimethylethyl)-4-hydroxybenzenepropanoic acid thiodi-2,1-ethanediyl ester
1-Butyl
Ethyl chloroacetate
TriMethylsulfoxoniuM iodide
2-AMino-6-hydroxy-8-Mercaptopurine
2,5-DibroMothieno[2,3-b]thiophene
Methyl 3-(3-broMophenyl)propanoate
2-BroMothiazole-5-carboxylic acid, 97%
Tetracaine Hydrochloride, USP
4-AMino-p-terphenyl
2-Methyl-2-adamantanol
(R)-(3-carboxy-2-hydroxypropyl)trimethylammonium chloride
Yttrium(III)-oxide
1,3-Cyclohexanediol
Oxalic acid dihydrate
Silicon carbide
3,5-Di-Tert-Butylbenzoic Acid
N-FMoc-O-tert-butyl-N-Methyl-L-serine, 97%
DL-Methionine sulfoxide, 98+%
Ethyl 3-Methylthiopropionate
1-Octanethiol
Ethyl 5-MethyliMidazo[1,2-a]pyridine-2-carboxylate
N-PHTHALOYL-L-PHENYLALANINE
2-Fluoro-4-Methylphenylboronic acid
ZINC CHROMATE
3-Pyrid-4-ylbenzoic acid, 97%
2,4-Dimethoxybenzenesulfonyl chloride
(S)-(+)-1-Chloro-2-methylbutane
(3-endo)-3-methoxy-8-azabicyclo[3.2.1]octane(SALTDATA: HCl)
ANGELIC ACID
Naftifine
5-Sulfoisophthalic acid
LINDANE
6-ForMyl-2-(Methylsulfanyl)nicotinonitrile
TetrabutylaMMoniuM sulfate solution
2,3,4,6-Tetra-O-benzoyl-D-Mannopyranose
2'-Deoxyguanosine hydrate, 99+%
6-AMino-5-nitro-2-picoline, 98%
Trimethylamine N-oxide dihydrate
4-AMidinobenzaMide Hydrochloride
N-fmoc-anthranilic acid
2-Acetamido-5-fluoropyridine
4-Chloropyrido[3,2-d]pyrimidine
2,4/2,6-Diaminotoluene
Thenoyltrifluoroacetone
2-Chloro-isonicotinoyl chloride
2-Fluoro-6-Methoxybenzonitrile, 98%
4-BroMo-N-tert-butyl-3-(trifluoroMethyl)benzenesulfonaMide
4-Fluorothioanisole ,98%
4-Amino-5-trifluoromethyl-4H-1,2,4-triazole-3-thiol
Tetramethyl-1,3-dimethoxydisiloxane
5-(4-fluorophenyl)-4H-1,2,4-triazol-3-amine(SALTDATA: FREE)
N-HYDROXYNAPHTHALIMIDE
Methyl 2-bromothiazole-5-carboxylate
P-NITRO-DL-PHENYLALANINE
Allyl heptanoate
4-(1H-IMidazol-1-ylMethyl)benzonitrile
1-Chloro-2-iodoethane (stabilized with Copper chip)
2-Methylpyridine-5-boronic acid, pinacol ester
FuMaric acid, 99+%
2-BroMo-1-[4-(difluoroMethoxy)phenyl]ethan-1-one, 97%
Diethylstilbestrol
DansylaMino-PITC [Fluorescent Coupling Reagent for EdMan Degradation]
Deuterated 1,2-dichlorobenzene
2,6-Dichlorophenol
fmoc-3-(4-pyridyl)-L-alanine
Daphnetin
Orexins ELISA Kit
P38MAPK ELISA Kit
Human FFAR1 ELISA Kit
Human DHDH ELISA Kit
ADM ELISA Kit
Rat GUSb (Glucuronidase, Beta) ELISA Kit
Chicken Heat Shock Protein 20,HSP-20 ELISA Kit
ProZ ELISA Kit
Human TP ELISA Kit
Apo-B100 ELISA Kit
LPM Agar Base
Gelatin Medium
GadoliniuM(III) 2,4-pentanedionate hydrate, REacton|r, 99.9% (REO)
Ammonium hexafluorosilicate
5-(4-MorpholinylMethyl)thiophene-2-boronic acid pinacol ester, 97%
2-Butynoic acid, 98%
2-Methyl-2-adamantanol
CUPRIC DICHROMATE
Vanadium carbide
3-nitrophenyl disulfide

SODIUM HYDROGEN FUMARATE
16-HYDROXYHEXADECANOIC ACID
Cbz-R-3-aMino-3-phenylpropan-1-ol
2-Cyanobenzeneboronic acid 1,3-propanediol ester
PotassiuM forMate, 99% (water less than 2%)
1-NaphthylMethylaMine
LanthanuM fluoride
(S)-Boc-4-chloro-β-Phe-OH
2,5-Diphenylbenzene-1,4-dicarboxylic acid
DIRECT PURE YELLOW 5G
Valencene
N,N'-Di(2-hydroxybenzyl)ethylenediamine-N,N'-diacetic acid monohydrochloride hydrate HBED
2,5-dichlorobenzothiazole
2-Iodo-1-methyl-1H-imidazole
2,7-Diphenyl[1]benzothieno[3,2-b][1]benzothiophene