B104 Cell:大鼠神经母传代贴壁细胞系 货号:GD-C6620583
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Phenyl benzoate, 99%
3-(Boc-aMino)piperidine
Polybutenes
1,4-DIBUTOXYBENZENE
3-Chloro-4-(3,5-diMethoxybenzyloxy)phenylboronic acid
DichlorMid
1,1-Bis(4-hydroxyphenyl)cyclohexane , 98%
3-Nitro-p-cresol
1-Isopropylnaphthalene
3-(allyloxy)benzaldehyde(SALTDATA: FREE)
3'-α-Sialyl-N-acetyllactosamine ,98%
Sodium selenite pentahydrate
Ethyl 4-(butylamino)benzoate
(S)-2-Benzothiazolyl (Z)-2-(2-aminothiazole-4-yl)-2-methoxycarbonylmethoxyiminothioacetate
1-Ethyl-3-methylimidazolium chloride
1,5-DiMethyl-1H-pyrazole-3-carboxylic acid
(+/-)-1,2-DiphenylethylenediaMine
1-(5-BroMopyridin-2-ylcarbonyl)pyrrolidine
Succinonitrile, 99+%
MES PotassiuM Salt, Biological Buffer
1-Ethyl-3-MethyliMidazoliuM BroMide
Bufotaline
N-ethyl-3-nitroaniline
1,6-Dibromohexane
ethyl caprate
1,2,3,4-CYCLOPENTANETETRACARBOXYLIC ACID
QUIN 2-AM
NipecotaMide
N-Methylanthranilic acid, 90+%
Ethyl L-nipecotate, 97%
4-[2-(Boc-aMino)ethyl]aniline, 97%
Glycyl-DL-alanine
2-BroMo-3-chloro-5-(trifluoroMethyl)pyridine
TERT-BUTYL (5-FORMYLPYRIDIN-2-YL)CARBAMATE
3-BroMo-5-chloropyridine 1-oxide
Phenyl phenylacetate
4'-Chloro-2'-Methylacetoacetanilide
O,O'-Bis(2-hydroxyethoxy)benzene, 98%
Sodium Dodecylbenzenesulfonate (soft type) (mixture) (62%, wetted with Water)
5-Methyl-2-(trifluoromethyl)furan-3-sulphonyl chloride
2-hydroxy-6-methoxyquinoline-3-carbaldehyde(SALTDATA: FREE)
3-Iodobenzylamine hydrochloride
5-ETHOXY-1H-INDOLE-3-CARBALDEHYDE
1,3-Dibromo-5-fluorobenzene
2-Methyltetrahydrofuran-3-one
2-Methoxyphenylacetic acid
Dibenzo[b,e][1,4]dioxin
N-t-Butyl 2-broMobenzaMide
TetrahexylaMMoniuM broMide, 99%
Neopentyl Glycol
alpha-Ionone
but-2-ynediamide
2-chloro-6-(trichloromethyl)pyridine
di-tert-butyl oxalate
4-amino-5-hydroxynaphthalene-1,7-disulfonic acid
4,5-METHYLENEDIOXY-2-NITROCINNAMIC ACID
4-ISOPROPYLPHENOXYACETIC ACID
3-Fluoro-2-Methoxybenzaldehyde
tert-Butyl(4-chlorobutoxy)diMethylsilane
4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium Chloride
4'-Hydrazino-2-stilbazole Dihydrochloride [for Fluorometry of alpha-Keto Acids]
4-Chloro-2-methoxybenzeneboronic acid
tert-Dodecyl Mercaptan (mixture of isomers)
3-CHLORO-4-METHYLPYRIDINE
2,3-Dichloro-5,6-dicyano-1,4-benzoquinone
2-(2-METHOXYLOXYPHENYL)-4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLANE
Choline hydroxide
3,4-DiMethylbenzene-1-carbonyl chloride
1-(4-BroMophenyl)-2,5-diMethylpyrrole
5-Chloro-2-(trifluoroMethoxy)phenylboronic acid
Cellulose triacetate
Zirconyl chloride hydrate, 99.99%, (trace Metal basis)
LithiuM Trifluoro(trifluoroMethyl)borate - DiMethyl Carbonate CoMplex
4-BroMo-3-hydroxy-2-naphthoic Acid
Sudan I
6-Ethoxypyridine-3-boronic acid
2,3-Dichloro-5-hydroxypyridine
alacepril
Porcine oxidized lowdensity lipoprotein,OxLDL ELISA Kit
Mouse Interleukin 2,IL-2 ELISA Kit
TNF-α ELISA Kit
Human GK5 ELISA Kit
IL13 ELISA Kit
CD40L ELISA Kit
IL-4 ELISA Kit
Human Brucella Antibody IgG,Brucella Ab IgG ELISA Kit
FABP3 ELISA Kit
Renin ELISA Kit
Brilliant Green Agar Medium
Trypticase Soy Sheep Blood Agar Base
Guaiacol
Periodic acid, 99%
Sulfosalicylic acid
2-Methylnaphthalene
Bufotaline
Fluorine
carbadox
xanthopterin
N-HEXANE-D14
1,4-DIHYDRO-2-METHYLBENZOIC ACID
2-Acetyl-4-BroMophenol
6-Methyl-3,5-heptadien-2-one, 97%
N-Benzyl-2-chloro-N-isopropylbenzaMide, 97%
1-Ethyl-3-MethyliMidazoliuM Hydrogen Sulfate
2-Hydroxy-2-(trifluoroMethyl)propionic acid
1,1′-Bis(phenylphosphino)ferrocene
N-HYDROXYNAPHTHALIMIDE SODIUM SALT
3,6-DibroMo-2-fluorobenzaldehyde
(1R,2R)-(-)-2-BenzyloxycyclopentylaMine
L-Alanine
4-Fluoro-3-methoxybenzaldehyde
4-[(Methylamino)sulphonyl]benzene boronic acid 97%
Phenethyl pivalate, 98+%