WM35 Cell:人黑色素瘤传代贴壁细胞系 货号:GD-C6620582
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Methyl-5-nitrobenzonitrile, 98%
Neopentyl glycol diMethacrylate
2,3-Dichloro-5-nitropyridine
trans-4-AMinocyclohexanol hydrochloride
2,2-DIMETHYL-5-THIOPHEN-3-YL-[1,3]DIOXANE-4,6-DIONE
3-METHYLCINNAMALDEHYDE
SodiuM peroxide
Hexyl Mercaptan
1-(Pyridin-2-yl)thiourea
2-{[5-(trifluoromethyl)-2-pyridinyl]amino}ethanol(SALTDATA: FREE)
6-Chloroquinazoline-2,4(1H,3H)-dione
N-(2,4-DINITROPHENYL)-L-ALANINE
1-Butaneboronic acid
2-Acetyl-3-methylpyrazine
3-PYRIDYLACETONITRILE
1-Fluoro-2-nitrobenzene
1,4-Dichloroanthraquinone
2-BroMo-1-(pyridin-2-yl)butan-1-one
Succinic anhydride, 99%
MeropeneM, USP
4-Methoxybutyl Acetate
stachydrine
2,5-Diaminopyridine
2-Nonanol
Dihexyl adipate
TRIS[N,N-BIS(TRIMETHYLSILYL)AMIDE]NEODYMIUM (III)
Cobalt Iron Oxide
Diethyl benzylphosphonate, 99%
Di-n-octyl sulfide, 97%
Naphthalene-2-sulfonic acid hydrate, 94%
FMoc-β-Cyclohexyl-L-alanine
(Perfluorohexyl)ethylene
1-Acetyl
TERT-BUTYL [(5-(AMINOMETHYL)PYRIDIN-2-YL]CARBAMATE
SodiuM benzenolate
2-Fluoro-6-hydroxyacetophenone, 98%
3,4-Dicyanothiophene
5-Fluoro-2-nitroanisole
Phenylthiohydantoin-threonine
Neurodye DiSBAC2(3), pure
2-Bromo-3-fluoropyridine
(S)-Cbz-Phenylalaninol
3,5-DIFLUOROCINNAMIC ACID
1-Methyl-4-(4,4,5,5-tetraMethyl-1,3,2-)
Di(tetrahydropyran-4-yl)aMine
5-BroMo-2-cyanopyridine
4-Ethoxy-2-(trifluoroMethyl)phenylboronic acid
DichloroMethylsilane, 99+%, AcroSeal
Zinc carbonate hydroxide, 58%, extra pure, powder, (53% Zn Min.)
FurfurylaMine
Allyl Phenyl Sulfone
Ferrocenylacetic acid
N-FMOC-L-2-AMINOPHENYLPENTANIOC ACID
3,5-difluoromandelic acid
1-Butyl-3-methylimidazolium bromide
ANTIMONY(III) METHOXIDE
4-Methoxy-2,3,5,6-tetrafluorophenylboronic acid
2-MethylaMinoMethyl-1,3-dioxolane
(1R,2R)-4-Cyclohexene-1,2-diaMine
trans-1-Methyl-4-carboxy-5-(3-pyridyl)-2-pyrrolidinone
2-Fluoro-5-(trifluoromethoxy)benzonitrile
Nicorandil
cis-Dichlorobis(pyridine)platinum(II), 99%
TERT-BUTYL N-(4-BROMOPHENYL)-CARBAMATE
Pyrogallol
4-Methyl-3-nitrophenol
2,6-DiaMinoheptanedioic acid;2,6-DiaMinopiMelic acid;Meso-DiaMinopiMelic acid;α,ε-DiaMinopiMelinic acid;(±)-2,6-DiaMinopiMelic acid;DL-2,6-DiaMinoheptanedioic acid;
5-Methyl-2-phenyl-2H-1,2,3-triazole-4-carboxylic acid
1-(4-BroMophenyl)-1,2,2-triphenylethylene
4-(N-EthylsulfaMoyl)-2-Methylphenylboronic acid
DicuMyl peroxide, 99%
4-(3,5-DiMethyl-1H-pyrazol-1-yl)aniline, 97%
HoMogentisic Acid gaMMa-Lactone
4-(1,1-DioxothioMorpholino)benzaldehyde
Dichloroethane
3,5-dibromo-2-chloro-4-methylpyridine
RAC-ETHYLENEBIS(4,5,6,7-TETRAHYDRO-1-INDENYL)DIMETHYLZIRCONIUM(IV)
benzyl [hydroxy-(4-phenylbutyl)phosphinyl] acetate
OLAb ELISA Kit
Mouse Interleukin 2,IL-2 ELISA Kit
TNF-α ELISA Kit
Human GNPAT ELISA Kit
IL1R2 ELISA Kit
CD40L ELISA Kit
Monkey Interleukin 4,IL-4 ELISA Kit
BAL ELISA Kit
Human FABP3 ELISA Kit
Renin ELISA Kit
Brilliant Green Phenol Red Lactose Agar
Bacillus Subtilis Optimization Medium
Sulfogaiacol
SodiuM peroxydisulfate, 98%
2-Hydroxy-5-Sulfobenzoic Acid
2-Methylnaphthalene, 97%
stachydrine
(R)-(-)-2-Phenylpropionic acid
diethyl isopropylidenemalonate
Ethyl 2-phenylbutyrate
N-(Methoxymethyl)-N-(trimethylsilylmethyl)benzylamine
1-Octanesulfonic Acid SodiuM Salt
Indole-7-carboxaldehyde, 98%
4-(Ethoxycarbonyl)butylzinc broMide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
(^+)-CaMphor, 96%
2-Nitroso-1-naphthol
1-OCTEN-4-OL
1,5-Difluoro-2,4-dinitrobenzene
Bis(tetraMethylcyclopentadienyl)nickel(II), 98+%
4-(2-AMinoethyl)tetrahydropyran, HCl
2-Thiophenecarbonyl chloride
3-Chloroperoxybenzoic Acid (contains min. 17% Water and min. 6% 3-Chlorobenzoic Acid)
Sodium sulfide, pure, 90+%
2-(4-Methoxyphenyl)pyrrolidine
Ethyl 7-chloro-4-hydroxy-8-methylquinoline-3-carboxylate