LIM1215 Cell：人结直肠癌复苏传代细胞系

LIM1215 Cell：人结直肠癌复苏传代细胞系 货号：GD-C6519377

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
为什么培养的细胞要及时传代？体外培养的细胞大多具有生长接触YZ的特性，也就是说当一个细胞被其他细胞包围的时候，它就会停止生长，及时传代后，细胞的生长得以继续。培养细胞生长减慢的原因在哪些?其解决办法有哪些？可能的原因有：更换了不同的培养液或血清；培养液中一些细胞生长必需成分如谷氨眈肢或生长因子等耗尽或缺乏或己被破坏；培养物中有少量细菌或真菌污染；试剂保存不当；比较新培养液与原培养液成分，比较新血清与旧血清等，找出其它可能的原因。解决办法：增加起始培养细胞浓度；让细胞逐渐适应新培养液；换入新鲜配制培养液；补加谷氨酷肢或生长因子等；用无抗生素培养液培养，如发现污染，去弃培养物，或用抗生素CJ；血清需保存在5℃到20℃; 培养液需在2-8℃避光保存;含血清完全培养液在2-8℃保存，并在2周内用完;分离培养物，检测支原体。冷冻管应如何解冻？取出冷冻管后，须立即放入37℃水槽中快速解冻，轻摇冷冻管使其在1-2分钟内大部分融化，特别注意，需残留一小块冰块，就可拿到超净工作台操作细胞，用75%酒精消毒冻存管，用新鲜培养基将细胞转移至培养瓶。另外冷冻管由液氮桶中取出解冻时，必须注意安全，预防冷冻管爆裂。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Ethyl 6-(trifluoroMethyl)nicotinate, 97%
2-Propanol-d8
5-Nitroanthranilonitrile
4-BroMo-2-fluorobenzonitrile
6-Methoxy-1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole hydrochloride
4-NITROBENZO-18-CROWN-6
3'-Bromoacetophenone
Methyl Red Sodium Salt
2-Bromo-5-methoxyaniline hydrochloride
Ammonium hypophosphite, for analysis, 97%
N-Hydroxy-3-nitro-benzamidine
Hexahydroisonicotinamide
2-Hydroxy-3-methoxybenzyl alcohol
1-Bromo-3-methylbutane
2-BroMo-1-(3,4-dihydro-2H-1,5-benzodioxepin-7-yl)ethan-1-one
3-(Pyrrolidin-1-ylMethyl)benzoic acid hydrochloride
4-(Decyloxy)benzoic Acid
4-(2-Chloroethoxy)benzaldehyde
2,6-DiMethylphenyl isothiocyanate, 98%
TriMethobenzaMide Hydrochloride, USP
D-(+)-Melibiose Monohydrate
6α,9-difluoro-11β,17,21-trihydroxypregna-1,4-diene-3,20-di...
Undecylbenzene
Pigment Blue 14
N-Benzylideneaniline
2-SULFOBENZOIC ACID HYDRATE
Ethanolamine
Bis(2,4,4-TriMethylpentyl)-Phosphinic Acid
4-NitrobenzaMidoxiMe, 97%
EthylaMine hydrochloride, 98%
Thifensulfuron
Thiostrepton froM StreptoMyces azureus
2-METHYLBENZYL MERCAPTAN, 97
N-Methyl 4-fluorobenzenesulfonaMide
4-EthylaMino-3-nitrophenylboronic acid, pinacol ester
(HEPTAFLUOROBUTYRO)PHENONE
4-Chloro-3-nitrocinnamic acid 98%
1-bromo-5-fluoropentane(SALTDATA: FREE)
2,4,6-Trimethyl-2,4,6-trivinylcyclotrisilazane
N,N-Diethylnicotinamide, 97%
Dimethyl-1，3-bis(carbomethoxy)-1-propen-2-yl phosphate
Hexyl salicylate
3-(4-METHYLPHENYL)PROPIONIC ACID
2-BroMopropanoic acid;(±)-2-BroMopropionic acid;DL-α-BroMopropanoic acid
2,5-Difluoro-4-Methoxybenzaldehyde
EModin
1H,1H-NonafluoropentylaMine
3-Methylthiophene-2-carbaldehyde oxiMe
3,5-Di(trifluoroMethyl)benzene-1-sulfonyl chloride, 97%
gamma-Linolenic acid
Hexyl ChloroforMate
4-bromo-3-nitropyridine
3-(methylamino)benzoic acid
2-undecylimidazole
2-ethylhexyl thioglycolate
1,4-Dibromo-2,5-dimethylbenzene
3-METHYLFORMANILIDE
2-BroMo-4-fluorophenol
3-(TrifluoroMethyl)benzhydrol, 97%
5-BroMo-4-(3,4-diMethylphenyl)pyriMidine
2,4,6-Tribromophenyl Hexanoate
2-Amino-3-methyl-5-nitropyridine
4-Butyl-2-methylphenyl isocyanate, tech
Dimethylammonium dichlorotri(?chloro)bis{(S)-(-)-2,2'-bis[di(3,5-xylyl)phosphino]-1,1'-binaphthyl}diruthenate(II) [NH2Me2][{RuCl((S)-xylbinap)}2(?Cl)3]
Phlorizin dihydrate
6-Chloro-2-methyl-3-nitropyridine
Magnesium bromide
n-Dodecyl-beta-D-maltoside
1-Heptene
4-(DiMethylaMino)-1-(triphenylMethyl)pyridiniuM Chloride
6,7-DibroMobenzo(1,4)dioxan
Thionyl chloride, 99.7%
Oxtriphylline, USP
1-Chlorononane
2-(1-Naphthylmethyl)-2-imidazoline hydrochloride
4-(diphenylphosphino)-Benzaldehyde
4-Bromobenzothiazole
kanamycin sulfate mixture of components A
Nephrin ELISA Kit
KISS1 ELISA Kit
Human SCD ELISA Kit
Human DPAGT1 ELISA Kit
Monkey PECAM1/CD31 (Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit
Rat interferon-inducible protein 16,IFI16/p16 ELISA Kit
ND-Ab ELISA Kit
Human PKCζ (Protein Kinase C Zeta) ELISA Kit
Human anti-PA Ab ELISA Kit
LCN2/NGAL ELISA Kit
Fluid Lactose Medium
0.01M PBS Broth
Ethylene-vinyl acetate copolymer
Phenol Red sodium salt
SodiuM 4-n-octylbenzenesulfonate, 98%, water less than 4%
2-Iodo-5-nitropyridine, 97%
6α,9-difluoro-11β,17,21-trihydroxypregna-1,4-diene-3,20-di...
8-hydrazinylquinoline hydrochloride
Acid Brown 50
DL-3-Amino-n-butyric acid

Decafluorobiphenyl
Boc-S-Benzyl-L-cysteine
(R)-(+)-2-Piperidinecarboxylic Acid
CeriuM(III) 2,4-pentanedionate hydrate
Pyruvate decarboxylase
3-Chlorothiophene
Calcitonin Gene Related Peptide FragMent 8-37 huMan
3-Ethoxyphenylboronic Acid
2-Decalone,Mixture of cis and trans
2-BROMO-3-THIOPHENECARBOXYLIC ACID 97
N-(Furan-2-ylMethyl) 2-broMo-5-fluorobenzaMide
1H-pyrazol-1-ylacetonitrile(SALTDATA: FREE)
(3-Bromo-4,5-dihydro-isoxazol-5-ylmethyl)carbamic acid tert-butyl ester, 97%
4'-Trifluoromethylbiphenyl-4-ylamine
3-Nitrophenylacetic acid, 99%