HUV-EC Cell：人脐静脉内皮复苏传代细胞系

HUV-EC Cell：人脐静脉内皮复苏传代细胞系 货号：GD-C6519198

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
6-BroMo-4-hydroxyquinoline-3-carboxylic acid, 96%
Fast Blue RR Salt
N-(BroMoMethyl)phthaliMide
6-(4-FLUOROPHENYL)IMIDAZO[2,1-B][1,3]THIAZOLE-5-CARBOXALDEHYDE
2,5-DICHLOROTOLUENE
2-FLUORO-3-(TRIFLUOROMETHYL)BENZYLAMINE
2-(4-METHYLBENZYLAMINO)PYRIDINE-5-BORONIC ACID PINACOL ESTER
2-Iodo-1H-perfluoroethane 97%
3-Bromo-2-fluoroaniline 99%
3-(Methylthio)thiophene
2,2-Dimethyl-4-pentenoic acid, 97%
(3S)-(+)-1-Benzyl-3-(methylamino)pyrrolidine
Methyl 4-(bromomethyl)benzoate
Citraconic anhydride
2-(1-Pyrrolidinyl)-5-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)pyridine
(5-Methylthien-2-yl)MethylaMine hydrochloride
3,6-Dichlorocarbazole
ZolMitriptan
VanadiuM(III) chloride, 97%
SodiuM Phosphate Tribasic, Dodecahydrate, Crystal, Reagent, ACS
Trifluoperazine Dihydrochloride
p-Tolylhydrazine hydrochloride
Potassium citrate monohydrate
4-Formylindole
2,6-bis(trifluoromethyl)pyridine
3,4-Dimethylisoxazol-5-amine
L-Phenylalanine Ethyl Ester Hydrochloride
4-Methoxyphenylacetic acid, 98+%
1-(2-Fluoro-4-nitrophenyl)azepane, 97%
Methyl 4-Methyl-3-nitrobenzoate, 99%
benzofenap
4-(6-ForMylpyridin-2-yl)benzonitrile
1-Triisopropylsilanyl-1h-pyrrole-3-boronic acid pinacol ester
Pentaerythritol tetrakis(2-Mercaptoacetate)
3-Hydroxy-5-(trifluoroMethoxy)phenylboronic acid
5-BroMobenzo[d]iMidazol-2-one
1,4-Dichloro-2-nitrobenzene
(3R)-(+)-3-Aminopyrrolidine
4-Methoxy-2-nitrophenol
Fmoc-d-asp(oall)-oh
4,5-Diamino-1-(2-hydroxyethyl)pyrazole sulfate
ETHANOL-D6
2-Chlorobutane
4-Chlorophenylboronic acid
2,3-Dichlorophenylacetone
Methyl 3,5-Dibenzyloxybenzoate
6-Chloroindole-2-boronic acid, pinacol ester
Bis(cyclopentadienyl)zirconiuM chloride hydride, 95%
BroMoacetaldehyde diMethyl acetal, 97%, stabilized
Indole-4-carboxaldehyde
Octatriacontane
Anthracene-9-carboxylic acid
4-4-Iodopyridine-2-carboxylic acid
BOC-D-GLU(OTBU)-OH
5-Chloro-2-tetralone
2-PROPYL-1-HEPTANOL
3-[Bis(2-hydroxyethyl)aMino]propyl-triethoxysilane solution
N-Acetyl-4-broMo-3-chloro-2-Methylaniline
3-AMino-2-chloro-5-iodopyridine
1-isopropyl-1H-pyrazol-4-amine(SALTDATA: HCl)
Indole-3-acetamide ,98%
1-Chloro-1-propene (cis- and trans- mixture)
2-Chloro-1-(4-chloro-phenyl)-ethanol
TEREPHTHALAMIDE
3,5-DICHLOROTHIOPHENOL
Dimethyl ether
2-Trifluoromethylphenylboronic acid
1-Boc-4-piperidinecarboxylic Acid
1-BroMo-10,12-pentacosadiyne
Ethyl 2-chlorooxazole-4-carboxylate
all-trans-Retinoic acid, 97%
3,4-Dihydro-2H-chroMen-2-ylMethanol, 97%
N-PhenylsucciniMide
ChloroMethylsulfonyl Chloride
1,4-Hexadiene, mixture of cis and trans
ethyl 6-oxo-1,6-dihydropyrimidine-4-carboxylate
methyl 2-oxo-1,2-dihydropyridine-4-carboxylate
2,3-Dichloro-6,7-dimethylquinoxaline
GAG ELISA Kit
Mouse PIAS1 (Protein Inhibitor Of Activated STAT 1) ELISA Kit
PGR ELISA Kit
Human UBE2C ELISA Kit
IL16 ELISA Kit
ACTN-1 ELISA Kit
ACV-AB ELISA Kit
Human MGL ELISA Kit
Human HBε1 ELISA Kit
Guinea pig nitric oxide,NO ELISA Kit
TGC Medium without Indicator ,Fluid
Acid Broth
LanthanuM(III) ethoxide, 95% (REO)
Fluorophosphoric acid, 70% w/w aq. soln.
5,7-Diiodo-8-quinolinol
2-Fluoro-4-BroMo-6-Nitroaniline
p-Tolylhydrazine hydrochloride
3-CYANO-7-METHOXYCOUMARIN
1-(4-NITROPHENYL)PYRROLIDINE
4-fluorobenzyl alcohol

NICKEL COPPER IRON MANGANESE
(5-MERCAPTO-1,3,4-THIADIAZOLE-2-YLTHIO)ACETIC ACID
5-BroMo-2-Chloropyridine
3-(3-Benzothienyl)-N-FMoc-L-alanine, 95%
Cholesteryl acetate, 97+%
Ethyl 4-aMinobutyrate hydrochloride
Tri-O-acetyl-D-galactal
4-Propylcyclohex-1-enylboronic acid
4-BROMOBENZHYDRAZIDE
N-BENZYLIDENEBENZENESULFONAMIDE
Ethyl 2-Methyl-4-oxo-2-cyclohexenecarboxylate
3,4,5-Trifluorobenzenesulfonyl chloride, 98%
Poly(vinyl alcohol), 75% hydrolyzed, approx. M.W. 2000
Fmoc-D-Gln(Trt)-OH
Sodium hydrosulfite, tech., ca. 85%