EL4 Cell：小鼠淋巴瘤复苏传代细胞系

EL4 Cell：小鼠淋巴瘤复苏传代细胞系 货号：GD-C6518748

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞常规培养传代流程(请严格遵照无菌操作)：1)吸出原培养瓶中的培养基，PBS缓冲液润洗细胞两次，加2-3ml 0.25%胰酶进行消化细胞(注意把握消化时间，通常控制在1-2min)；2)镜下观察消化情况，在细胞边缘缩小，贴壁松动时(不建议消化到细胞漂浮)去掉胰酶，加6-8ml完全培养基，轻轻吹打细胞层，尽量把细胞层吹落，吹散；3)取部分细胞悬液转移到新的培养皿/瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养；4)注意培养基PH值变化情况，定期换液(每周2-3次)，待细胞密度达到80%以后重复1项操作或者冻存；特别注意：(如使用公共实验室或者初次接触细胞培养，建议添加双抗培养)1)收到细胞后请尽快更换为含15%血清的新鲜培养基，如因特殊情况需要继续使用原瓶，请在原瓶培养基中额外添加10%的血清，(原瓶培养基的继续使用时间Z长不宜超过72小时)；2)贴壁细胞收到当天切忌立刻消化，请将细胞换液后放置培养箱孵育到第二天再做消化传代，请优先选择直径6cm的培养皿进行传代培养；3)如签收时出现培养瓶壁破裂，漏液等情况请及时做好照片记录并联系实验室。
细胞培养实验中常见问题总结：1)一般客户拿到细胞后，应该注意什么？客户收到细胞后先不开盖，放在培养箱静置若干小时后(看细胞密度而定)在倒置显微镜下观察细胞生长情况，并对细胞进行不同倍数拍照(建议受收细胞后观察培养基的颜色和是否有漏液情况，显微镜下拍细胞100X，200X各一张)，排除细胞本身污染的情况；收到细胞未开封，出现污染状况我们负责免费发送一株细胞。收到细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。细胞消化液建议使用PBS配制，慎用Hanks液配制；2)快递细胞多久能到，是寄冻存的细胞还是复苏好的细胞？我们采用快递发货，一般外地2--3天，寄细胞前请确认当地温度，如果气温低于4度的，则采用邮寄冻存细胞；3)可否使用与原先培养条件不同之培养基？不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活；4)可否使用与原先培养条件不同之血清种类？不能。血清是细胞培养上一个极为重要的营养来源，所以血清的种类和品质对于细胞的生长会产生极大的影响。来自不同物种的血清，在一些物质或分子的量或内容物上都有所不同，血清使用错误常会造成细胞无法存活。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
6,6'-DibroMo-2,3'-bipyridine, 95%
CalciuM oxide
SodiuM broMate
GadoliniuM galliuM isopropoxide, 99.9% (Metals basis), 25% w/v in isopropanol
o-Toluoyl chloride
3-Methyl-4-nitrobenzyl broMide
4-BroMo-1-(difluoroMethyl)-2-fluorobenzene
Ethyl picolinate, 99%
1-Chloroundecane
Poly(4-methylstyrene), average M.W. ca. 70000
Potassium cyclohexyltrifluoroborate
2,3,4-Trifluorotoluene
5-(BROMOACETYL)-3-PHENYLISOXAZOLE
BRUCINE HYDROCHLORIDE
2-FLUORO-6-(TRIFLUOROMETHYL)BENZYL BROMIDE
2-Chloro-5-nitro-3-picoline
DiMethylMalonic Acid
5-BroMo-4-sec-butylpyriMidine
TelluriuM, 99+%, reagent, powder
MethylthyMol Blue SodiuM Salt, Reagent, ACS
6-Chlorouracil
5,5-dibromobarbituric acid
5-methy1-2,3-dihydroinden-1-one
N-Morpholino-1-cyclohexene
4-methyl-2-nitroanisole
(S)-(-)-N-ISOPROPYL-1-PHENYLETHYLAMINE HYDROCHLORIDE
2-AMino-5-Iodopyridine
DodecacarbonyltetrairidiuM, 98%
Ethyl thiophene-2-carboxylate, 98+%
DiethoxyMethylphenylsilane, 97+%
pH standard－Borax
ETHYL 3-BENZOYLACRYLATE
DIBENZO-15-CROWN-5
Sulfonazo III tetrasodiuM salt
4,4'-BIS(DIMETHYLAMINO)BENZIL
3,5-DibroMobenzyl broMide
5,7-Dimethylisatin, 95%
3-Aminobenzyl alcohol
2-Fluoro-4-iodobenzaldehyde
4-Isopropylpyrimidine-5-boronic acid 95%
PHOSPHORUS PENTABROMIDE
(S)-3-AMINO-3-(4-CHLORO-PHENYL)-PROPIONIC ACID
3-(TERT-BUTYL)-1-METHYL-1H-PYRAZOLE-5-CARBONYL CHLORIDE
TetrapentaaMMoniuMiodide;
9-FluorenylMethyl ChloroforMate
4-Chloroaniline-3-sulfonic Acid
3-Isobutoxy-5-Methylphenylboronic acid
DL-Leucine, 99+%
2-Cyclopentylphenol, 98+%
Acetaldehyde AMMonia TriMer
Ethylene Glycol Monovinyl Ether (stabilized with KOH)
1,4-Butane sultone
2,4-Bis(hexyloxy)phenylboronic acid pinacol ester
2-Propenyl-2-propynylcarbamic acid tert-butyl ester
O-benzotriazol-1-yl-tetramethyluronium hexafluorophosphate
(TRIETHYLSILYL)ACETYLENE
LITHIUM ARSENATE
2-BENZOYL-1,3-INDANEDIONE
(1S,2R)-1-PHENYL-2-(1-PYRROLIDINYL)PROPAN-1-OL
DiMethyl fluoroMalonate
3-(Dimethylcarbamoyl)benzeneboronic acid
3,4-Dihydroxy-5-nitrobenzaldehyde
1-Ethyl-3-guanidinothiourea Hydrochloride
2,6-Bis(4-azidobenzylidene)-4-methylcyclohexanone (wetted with ca. 30% Water, containing 25g on a dry weight basis)
6-METHYLCHROMONE-2-CARBOXYLIC ACID
4-(Trifluoromethyl)benzaldehyde
L-CYCLOSERINE
6-Chloronicotinic acid
1-(tert-Butyl) 2-Methyl 1H-indole-1,2-dicarboxylate
Phenyl 1,4-Dihydroxy-2-naphthoate
3-BroMo-5-phenyl-1,2-oxazole
SodiuM forMaldehydesulfoxylate dihydrate, 98%
L-Ornithine Hydrochloride, Ultrapure
2-Chlorobenzotrifluoride
2-bromo-1-(4-fluorophenyl)ethan-1-one
[(4-methoxyphenyl)ethynyl](trimethyl)silane
2,3-dichloropropan-1-ol
3,4-dihydro-2-ethoxy-2H-pyran
PDGF ELISA Kit
IL-1β ELISA Kit
Human ANA ELISA Kit
MASP ELISA Kit
Chicken Transforming Growth factor β2,TGF-β2 ELISA Kit
COLⅩⅦ ELISA Kit
Monkey MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
Human FOXC2 (Forkhead Box Protein C2) ELISA Kit
STAT4 ELISA Kit
OX-B ELISA Kit
Pyridoxine Y medium
LAPT Broth
LY 171883
Dioctyl Sebacate
5-FLUORO-DL-TRYPTOPHAN
2-Methylfuran, 98+%, stab.
5,5-dibromobarbituric acid
Silicon Tetrafluoride
4-Aminocyclohexanol
Isobutyl benzoate

3-Cyanomethylthiophene
4-Methoxybenzyl cyanide
2,6-Difluoroaniline
1-(3-TrifluoroMethylphenyl) Monohydrochloride, 98%
1-[3-(TrifluoroMethyl)benzyl], 97%
3-ThiopheneMalonic acid
Hexachlorophene solution
ISOAMYLAMINE
2-(METHYLTHIO)THIAZOLE
PotassiuM 4-trifluoroboratoMethylthioMorpholine
2-(4,4-DiMethyl-4,5-dihydro-2-oxazolyl)quinoline
Iron(III) pentane-2,4-dionate
4,5-Dibromothiophene-2-carboxylic acid
N-Carbobenzoxy-L-methionine
Vanadium(IV) oxide, 99+%